ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (7): 645-648.doi:

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Studies of Insulin-like Growth Factor-Ⅰ(IGF-Ⅰ)Gene Polymorphism Analysisand Its Associations with Body Weight and Carcass Traits in Shouguang Chicken

MIN Ling-jiang;PAN Qing-jie; CHEN Hong; LEI Chu-zhao; LI Mei-yu;SUN Guo-qiang
  

  1. 1. College of Animal Science and Technology, Northwest A & F University，Yangling 712100,China; 2. College of Animal Science and Technology, Laiyang Agricultural College, Laiyang 265200, China； 3. Institute of Cellular and Molecular Biotechnology,Xuzhou Normal University, Xuzhou 221116, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-07-25 Published:2005-07-25

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Abstract: Genetic variation of the 5′ region of the IGF-Ⅰgene in 200 Shouguang chickens were detected by PCR-RELP. Three genotypes AA，AB and BB were identified after the PCR products were cut by PstⅠrestriction enzyme and their frequencies were 0.335，0.485 and 0.180, respectively. They were in Hardy-Weinberg equilibrium at the gene locus by χ2 test（P＞0.05）. The associations were observed between genotypes and carcass traits including semieviscerated weight and breast muscle weight (P＜0.05). The results of the least squares means showed that a significant different effect between genotype BB and AB or AA was found in semieviscerated weight of Shouguang chickens (P＜0.05), while the significant difference was found only in breast muscle weight of the male chickens and only in liver weight of female chickens (P＜0.05). In addition, genotype BB tended to express a higher body weight in 10 weeks, semi-eviscerated weight and breast muscle weight than the other genotypes in Shouguang chickens. These results may be applied to marker assisted selection.