ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (5): 482-485.doi:

• 兽医 • Previous Articles     Next Articles

Research on Detection of Tetracycline Resistance Gene(tetC) of Pathogenic Salmonella from Swine by PCR and Nucleic Acid Probe

DAI Min; WANG Hong-ning; WU Qi   

  1. 1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014,China; 2. Key Laboratory for Molecular Biology and Biopharmaceuticals, Mianyang Normal University, Mianyang 621000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-05-25 Published:2005-05-25

PDF (PC)

666

Abstract: The detection of tetracyclines-resistance of 16 Samonella isolates showed the resistance rate was 100%. The tetC gene of 16 salmonella strains was amplified by polymerase chain reaction. 12 strains with this gene were located on plasmids. Compared with the result of the drug-susceptibility tests, the method of PCR showed 75% concordance in positive rate. The probe of tetC gene was labelled with photobiotin，and 16 pathogenic Salmonella strains from swine were detected by colony hybridization in situ. The result indicated that 13 strains were positive, 3 strains were negative. Compared with the result of PCR, the method of the tetC probe showed 93.75% concordance in positive rate. In this study, two methods for detecting tetC gene were established by using PCR and nucleic acid probe, and they could be applied to the molecular epidemiology surveillance of tetracyclines-resistance.