Abstract: The major object of this paper is to isolate and culture bovine embryonic germ (EG) cells derived from primordial germ cells (PGCs) as well as to identify the EG cells from various aspects. Some factors influencing the efficiency of the isolation and culture bovine EG cells have been discussed. When co-culture the EG cells with somatic cells, there will be some colony in primary culture, which maybe account for homologous somatic cells can sustain the EG survive and proliferation. When we use the tissue culture method, the colony of EG cells is very less and EG cells cannot proliferate effectively, as well as the passage culture. With 0.125% trypsinase + 0.02% EDTA disrupt gonad, comparing effect of different dispersal methods on the passage of the EG cells, the method of “digest + blowing disperse” was fine, which was simple and can save more time and labours and better to keep the activity of the EG cells. An Immunohistochemistry assay was carried out to characterize the colonies of bovine primory EG cells, and expression of stage-specific embryonic antigen SSEA-1, 3, 4 was detected, but the expression was feeble. The EG cells can differentiate into fibroblast-like cells, epithelia-like cells and embryoid body.