Abstract: Two fragments of 985 bp and 934 bp were amplified by PCR technique from the DNA genome of PRV Guangxi B and W wild strains according to the published gE gene of PRV Rice strain. These PCR products were purified by agrose gel, then were cloned into pMD18-T vector and transformed into E. coli DH5α. Four positive recombinants (pMD18-T GXB1, W1 and pMD18-T GXB2, W2) were obtained. The recombinants were determined by PCR and digestion of HindⅢ and EcoRⅠ. Then the inserts fragments were sequenced. The results revealed that the gE genes were both composed of 1 743 base pairs and coded for 580 amino acids. The homologe of nucleotide sequence and deduced amino acid sepuence between GXB and GXW stvains were 99.4%,99.1% respectively.GXB shared 98.0% and 95.7% homology of nucleotide sequence and deduced amino acid sequence with Rice strain,GXW shared 97.8% and 95.2% homologe respectively. The homology of nucleotide and deduced amino acid sequence between the two strains from Guangxi and other domestic PRV strains were 98.4%~99.5% and 97.4%~99.1% respectively. Phylogenetic tree analysis showed there was a high relationship between GXB, W strains and Ea strain.