ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (8): 1447-1454.doi: 10.11843/j.issn.0366-6964.2015.08.022

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Effect of Aflatoxin B1 on Pathological Changes of Immune Organs in Broilers

YU Zheng-qiang1# ,CHEN Jin2# ,PENG Xi1* ,FANG Jing1,CHEN Ke-jie1,HE Yang1   

  1. (1.College of Veterinary Medicine,Sichuan Agricutural University,Chengdu 611130,China;2.Animal Husbandry Bureau of Xichang,Xichang 615000,China)
  • Received:2014-11-12 Online:2015-08-23 Published:2015-08-23

Abstract:

The aim of the current study was to investigate the histopathological and ultrastructural changes caused by dietary AFB1 in broilers.One hundred one-day-old avian male broilers were randomly divided into four equal groups and were fed for 21 days as follows:a control diet and three AFB1 addition diets containing 0.15,0.3 and 0.6 mg•kg-1 AFB1,respectively.The results showed that the relative weight of the three organs were lower than those of the control group (P<0.05).Histopathologically,in the AFB1 groups,there were increased nuclear debris around the reticulocytes in the cortex of the thymus;the number of lymphocytes was decreased in the medulla,and increased nuclear debris can be observed in the lymphoid follicle of the Bursa of Fabricius;more nuclear debris appeared around lymphoid follicles and lymphatic sheath in the chicken spleens.The ultrastructural changes were mitochondria swelling and increased apoptotic cells characterized as chromatin margination in the lymphocytes of the three immune organs.These results indicated that when the contents of dietary AFB1 were from 0.15 to 0.6 mg•kg-1,the development of immune organs could be inhibited,and the major lesions of the three immune organs were the decrease of lymphocytes and the increase of nuclear debris.

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