ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (6): 943-952.doi: 10.11843/j.issn.0366-6964.2014.06.012

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Study of Nitrogen Metabolism and Net Protein Requirement for Maintenance of Dorper×Thin-tailed Han Crossbred Breeding Ewes

LOU Can,JIANG Cheng-gang,MA Tao,JI Shou-kun,CHEN Dan-dan,TU Yan,ZHANG Nai-feng,DIAO Qi-yu*   

  1. (Key Laboratory of Feed Biotechnology of Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081, China)
  • Received:2013-10-08 Online:2014-06-23 Published:2014-06-23

Abstract:

This experiment was conducted to investigate the nitrogen apparent digestibility of different feeding levels and net protein requirement for maintenance of Dorper × Thin-tailed Han crossbred ewes in different physiological periods.According to the weight,fifteen ewes with synchronous estrus and artificial insemination were assigned into three groups,one group fed ad libitum(100%),and the other two groups fed eighty percents(80%) or sixty percents(60%) of ad libitum,and another nine non-pregnant ewes were assigned into blank control group.Digestibility trials was performed in the non-pregnant condition and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation,to determine the metabolic parameters of nitrogen and net protein requirement for maintenance.The apparent digestibilities of nitrogen in the non-pregnancy and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation were 45.21%-51.34%;59.71%-64.14%,69.98%-71.91%,67.34%-68.80%;73.72%-82.71%,72.79%-80.51%,73.57%-76.47%,respectively,which only in the non-pregnancy,mid- and late-lactation had significant differences (P<0.01).The regression equations between nitrogen intake (N intake,g•kg-1 W0.75•d-1) and nitrogen retention (RN,g•kg-1 W0.75•d-1) in the non-pregnancy and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation were RN=0.341 5NI-0.269 7;RN=0.489 5NI-0.303 7,RN=0.384 5NI-0.323 4,RN=0.457 4NI-0.496 2;RN=0.252 1NI-0.338 0,RN=0.282 1NI-0.313 6,RN=0.286 3NI-0.298 2(P<0.01),respectively.The net nitrogen requirements for maintenance of ewes in the non-pregnancy and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation were 269.7,303.7,323.4,496.2,338.0,313.6 and 298.2 mg•kg-1 W0.75•d-1 ,respectively.

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