三鸡种白细胞介素1β基因多态与血清中白细胞介素1含量相关性分析

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (2): 162-166.

三鸡种白细胞介素1β基因多态与血清中白细胞介素1含量相关性分析

王存波¹，常国斌¹，徐琪¹，王克华^2，3，雷黎丽¹，周琼¹，廖菁¹，陈蓉¹，胡国顺¹，龚琳琳¹，陈国宏¹*

1. 扬州大学动物科学与技术学院，扬州 225009；2. 中国农业科学院家禽研究所，扬州 225003；3. 扬州翔龙禽业发展有限公司，扬州 225261

收稿日期:2009-01-16 修回日期:1900-01-01 出版日期:2010-02-24 发布日期:2010-02-24
通讯作者: 陈国宏

Correlation Analysis between Single Nucleotide Polymorphism of the IL1βGene and IL1 Content in Serum in Three Chicken Breeds

WANG Cunbo1，CHANG Guobin1，XU Qi1，WANG Kehua2，3，LEI Lili1，ZHOU Qiong1，LIAO Jing1，CHEN Rong1，HU Guoshun1，GONG Linlin1，CHEN Guohong 1*

1. College of Animal Science and Technology, Yangzhou University, Yangzhou225009, China; 2. Institute of Poultry Science, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China；3. Xianglong Poultry Development Co., Ltd.of Yangzhou, Yangzhou 225261, China

Received:2009-01-16 Revised:1900-01-01 Online:2010-02-24 Published:2010-02-24
Contact: CHEN Guohong

摘要/Abstract

摘要： 本研究旨在通过检测鸡白细胞介素1β(interleukin1β，IL1β)基因部分区域单核苷酸多态位点，以期查找与血清中IL1含量相关的遗传标记。根据红色原鸡IL1β基因序列设计2对引物，利用PCR及单链构象多态性(SingleStrand Conformation Polymorphism，SSCP)方法对如皋鸡、文昌鸡和安卡鸡进行单核苷酸多态性(Single nucleotide polymorphism, SNP)分析，并利用试剂盒ELISA法对56日龄血清IL1含量进行检测。结果表明血清中IL1含量安卡鸡极显著高于如皋鸡（P<0.01）和文昌鸡（P<0.001）。IL1β基因476位点发生G→A突变、482位点发生T→C突变、1 215位点发生C→T突变、1 507位点发生T→C突变。对多态位点和IL1含量作关联分析，只发现1 215位点与IL1含量有关，TT基因型个体IL1含量显著高于CC型个体。本研究初步表明IL1β基因1 215多态位点可以作为抗病育种中的候选遗传标记。

关键词: 白细胞介素1, 白细胞介素1β基因, 单核苷酸多态性, 鸡

Abstract: The aim of the present study was to detect single nucleotide polymorphism (SNP) in some areas of IL1β gene of chicken to find genetic marker influencing on IL1 content in serum. Two pairs of primers were designed according to the sequence of IL1β gene of red jungle fowls, and the SNP of Rugao chicken(RG), Wenchang chicken(WC), and Anka chicken(AK) were analysed by PCRSSCP, meanwhile, the IL1 content in serum were detected at 56 days with reagent kit by ELISA. The results showed that the IL1 content in serum of AK was significantly higher than that of RG(P<0.01) and WC(P<0.001). The mutation were found at four sites in all three breeds, and they were mutation G→A at 476 site, mutation T→C at 482 site, mutation C→T at 1 215 site and mutation T→C at 1 507 site. Then, the correlation between polymorphic sites and content of IL1 were analysed, and only 1 215 site had relationship with IL1 content in serum, which showed that chicken with TT genotype had significantly higher IL1 content in serum than those with CC genotype. This study indicated that the 1 215 polymorphic site could be a genetic marker for IL1content in serum.

Key words: interleukin1, IL1β gene, SNP, chicken

王存波;常国斌;徐琪;王克华;雷黎丽;周琼;廖菁;陈蓉;胡国顺;龚琳琳;陈国宏. 三鸡种白细胞介素1β基因多态与血清中白细胞介素1含量相关性分析[J]. 畜牧兽医学报, 2010, 41(2): 162-166.

WANG Cunbo;CHANG Guobin;XU Qi;WANG Kehua;LEI Lili;ZHOU Qiong;LIAO Jing;CHEN Rong;HU Guoshun;GONG Linlin;CHEN Guohong . Correlation Analysis between Single Nucleotide Polymorphism of the IL1βGene and IL1 Content in Serum in Three Chicken Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(2): 162-166.

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