畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (2): 251-254.

• 研究简报 • 上一篇    

表现腺胃炎的蛋用型鸡J亚群白血病病毒的分离与鉴定

孙淑红,柴家前,王波,孙洪磊,王晓云,崔治中*
  

  1. 山东农业大学 动物科技学院/动物医学院,泰安 271018
  • 收稿日期:2009-08-05 修回日期:1900-01-01 出版日期:2010-02-24 发布日期:2010-02-24
  • 通讯作者: 崔治中

Subgroup J Avian Leukosis Viruses Isolated from Eggtype Chickens with Proventriculitis

SUN Shuhong, CHAI Jiaqian, WANG Bo, SUN Honglei, WANG Xiaoyun, CUI Zhizhong*   

  1. College of Animal Science and College of Veterinary Medicine,Shandong Agricultural University, Taian 271018, China
  • Received:2009-08-05 Revised:1900-01-01 Online:2010-02-24 Published:2010-02-24
  • Contact: CUI Zhizhong

摘要: 从表现腺胃炎的尼克珊瑚粉商品代蛋鸡中分离到J亚群白血病病毒(ALVJ)。将病料或鸡白细胞接种于CEF,培养12 d,分别采用单克隆抗体间接免疫荧光试验检测,结果10只鸡中有9只鸡分离到ALVJ,其中有4只鸡还存在与禽网状内皮增生病病毒(REV)的共感染。通过PCR扩增gp85基因,与已发表的20株ALVJ进行同源性比较。结果表明,与来自白羽肉鸡的HPRS103的同源性为97.8%,而与来自蛋用型鸡的SD07LK1株的同源性为93.0%。本研究发现,在某些仅仅发生腺胃炎的鸡也可能普遍存在ALVJ感染,再次显示了腺胃炎病料中病毒感染的多样性。ALVJ可能成为致腺胃炎的病原之一,但其致病作用有待进一步研究。

关键词: 腺胃炎, 蛋用型鸡, J亚群禽白血病病毒

Abstract: Subgroup J avian leukosis virus (ALVJ) was isolated from both provetriculus suspensions and blood samples of 9 in 10 laying hens only with proventriculitis, and coinfection of ALVJ with reticuloendotheliosis virus was also confirmed in 4 chickens. The gp85 genes of ALVJ isolates were amplified by PCR, sequenced and compared to 20 reference ALVJ strains. Sequence comparisons indicated that the provetriculus isolate had 978% identity with the HPPS103 strain isolated from white meattype chickens and 93.0% identity with SD07LK1 strain from eggtype chickens. The result demonstrated that ALVJ infection was common in some chickens with only proventriculus, further indicating the diversity of virus infections associated with proventriculitis. ALVJ may be take part in pathogenesis of provetriculitis in some chickens, but its pathogenic role needs to be further studied.

Key words: proventriculitis, eggtype chickens, subgroup J avian leukosis viruses