关键词: 雏鹅新型病毒性肠炎病毒强毒, 人工感染, 超微结构变化, 病毒形态发生

Abstract: The morphogenesis of new gosling type viral enteritis virus (NGVEV) in duck embryo and the ultrastructural changes of host cells were observed and analysed by using electron microscopic techniques. 10 days old duck embryos were infected experimentally with NGVEV. Chorioallantois,intestine, heart, liver, brain and muscular stomach were collected at different times. The investigation showed that the typical adenovirion could be found in chorioallantois, intestine, heart, liver, brain and muscular stomach at the different infection stages. The virus particles were round in shape with diameter of 60-70 nm. There are three significant morphogenetic envents of NGVEV according to our observation: entry, virion morphogenesis and release. The virion entered the cell by attaching to cytoplasmic membrane and replicated,matured in the nucleus.Finally virions released into extracellular space through broken of cytoplasmic membraneThe target cells that virus mainly attacked were the chorioallantois epithelial cells, intestinal epithelial cells, smooth muscle cells of intestine, fibrocytes, hepatocytes ,muscular stomach mucus membrane epithelial cells and cardiac muscle cells The target organelles that virus mainly attacked were rough endoplasmic reticulum (RER) and mitochondria (Mi) RER in the cytoplasm expanded heavily during infection Mitochondria in the chorioallantois epithelial cells were ultracondensed and aggregated into compact clusters, but mitochondria in other cells were inflated and disaggregated after NGVEV infection NGVEV could induce apoptosis in host cells The morphological changes of apoptosis including the apoptotic cells shrank, chromatin condensation and margination, and the formation of apoptotic bodies

Key words: new gosling type viral enteritis virus, artificial infection, ultrastructural changes, virus morphogenesis