畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (1): 12-17.

• 遗传繁育 • 上一篇    下一篇

鸡肠道SGLT1和GLUT2 mRNA表达的组织特异性研究

王修启;谭会泽;束 刚;苏海林;陈黎龙;江青艳;冯定远   

  1. 华南农业大学动物科学学院,广州 510642
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-01-25 发布日期:2006-01-25

Study on Tissuespecific Expression of SGLT1 and GLUT2 mRNA in Chicken Intestine

WANG Xiu-qi;TAN Hui-ze;SHU Gang;SU Hai-lin;CHEN Li-long; JIANG qing-yan; FENG Ding-yuan   

  1. College of Animal Science, South China Agricultural University,Guangzhou 510642, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-01-25 Published:2006-01-25

摘要: 运用相对定量RT-PCR方法,研究不同肠段Arbor Acre (AA)肉鸡肠道葡萄糖吸收转运主要载体SGLT1和GLUT2 mRNA表达的组织特异性。结果发现,随着肠道空间位置的后移,SGLT1 mRNA的表达量逐步降低。十二指肠SGLT1 mRNA的丰度比结直肠高76.19%,差异极显著(P<0.01);而空肠和回肠SGLT1 mRNA的表达量分别比结直肠高42.86%和38.10%,差异不显著(P>0.05),但有提高的趋势(P值分别为0.06和0.07)。十二指肠与空肠和回肠相比,SGLT1 mRNA的表达量虽然分别高23.33%和27.59%,但差异不显著(P值分别为0.18和0.10)。相对定量分析表明,十二指肠和空肠GLUT2 mRNA丰度非常接近,差异不显著(P>0.05)。定性研究显示,十二指肠与空肠 GLUT2 mRNA丰度高于回肠和结直肠。鸡肠道SGLT1和GLUT2 mRNA表达的组织特异性之生理功能,有待于进一步研究。

关键词: 肠道, SGLT1 mRNA, GLUT2 mRNA, 组织特异性

Abstract: The objective of this study was to investigate tissue-specific expression of SGLT1 and GLUT2 mRNA in different intestine segments of Arbor Acre (AA) chicken by relative quantative RT-PCR. The results showed that the expression of SGLT1 mRNA decreased gradually along the downward intestine. SGLT1 mRNA abundance of duodenum, jejunum and ileum was 76.19% (P<0.01), 42.86%(P=0.06) and 38.10%(P=0.07) higher than that of colorectum, respectively. And SGLT1 mRNA expression of duodenum was higher 23.33%(P=0.18) and 27.59%(P=0.10) than that of jejunum and ileum, respectively. The GLUT2 mRNA expression of duodenum was close to that of jejunum, the difference was not significant (P>0.05). Single gene PCR results indicated the GLUT2 mRNA abundance of duodenum and jejunum were much more higher than that of ileum and colorectum. Further researches should be done on the physiological function of tissue-specific expression of SGLT1 and GLUT2 mRNA in chicken intestine.

Key words: intestine, SGLT1 mRNA, GLUT2 mRNA, tissue-specific