蔗糖对板青颗粒薄层色谱鉴别影响的发现和消除

doi:10.11843/j.issn.0366-6964.2019.08.020

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (8): 1702-1708.doi: 10.11843/j.issn.0366-6964.2019.08.020

蔗糖对板青颗粒薄层色谱鉴别影响的发现和消除

杨新¹, 李硕¹, 李亚威¹, 陈薇¹, 范琦¹, 郑春红¹, 高经纬¹, 黄琦², 周锐², 刘洁^1*

1. 武汉回盛生物科技股份有限公司, 湖北省兽药工程技术研究中心, 武汉 430042;
2. 华中农业大学动物医学院, 科技部动物疾病防控国际联合研究中心, 武汉 430070

收稿日期:2019-03-01 出版日期:2019-08-23 发布日期:2019-08-19
通讯作者: 刘洁,主要从事药学研究,E-mail:575651807@qq.com
作者简介:杨新(1991-),男,湖北随州人,硕士,主要从事天然产物化学生物活性效应研究,E-mail:361159566@qq.com
基金资助:
国家重点研发计划政府间国际科技创新合作重点专项（中英政府间合作项目2018YFE0101600）

Characterization and Elimination of the Effect of Sucrose on Ban Qing Granules TLC Identification

YANG Xin¹, LI Shuo¹, LI Yawei¹, CHEN Wei¹, FAN Qi¹, ZHENG Chunhong¹, GAO Jingwei¹, HUANG Qi², ZHOU Rui², LIU Jie^1*

1. Hubei Province Veterinary Drug Engineering Technology Research Center, Wuhan HVSEN Biotech Co., Ltd., Wuhan 430042, China;
2. International Research Center for Animal Disease, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

Received:2019-03-01 Online:2019-08-23 Published:2019-08-19

摘要/Abstract

摘要： 本研究旨在查明板青颗粒薄层色谱鉴别时出现供试品的精氨酸斑点相对于对照品精氨酸斑点滞后现象的原因，并探讨该鉴别方法的改进措施。用现行的《中华人民共和国兽药典》2015年版二部规定的方法进行板青颗粒薄层色谱鉴别实验，用斐林试剂检测供试品溶液制备过程中蔗糖水解生成的还原糖，对供试品溶解和蒸干等样品处理方法进行改进。结果发现，板青颗粒供试品溶液制备过程中，蔗糖水解生成的还原糖可以与精氨酸发生美拉德反应，导致薄层色谱斑点滞后；用95%乙醇替代稀乙醇、用自然挥发干燥代替加热蒸干进行供试品溶液的制备，以减少其中蔗糖的含量和还原糖的产生，可以有效解决斑点滞后问题。板青颗粒中的蔗糖在供试品溶液制备过程中水解生成的还原糖与精氨酸发生美拉德反应，是导致薄层色谱鉴别斑点滞后的原因，改变供试品溶液制备方法可以有效消除蔗糖对板青颗粒薄层色谱鉴别的影响，解决了斑点滞后问题，对板青颗粒质量标准的修订提供了科学依据。

关键词: 板青颗粒, 薄层色谱鉴别, 斑点滞后, 方法改进, 蔗糖, 美拉德反应

Abstract: The aims of this study were to find the reason of the arginine spots lag in the thin layer chromatography (TLC) identification of Ban Qing granules, and to improve this identification method. The TLC identification test was carried out as described in the current version of ChVP2015 Part Ⅱ - Ban Qing granules. Sucrose hydrolysis during TLC sample preparation process was tested by Fehling's solution. TLC sample preparation process was modified by changing the dissolution and heating steam drying methods. The results showed that the arginine spots lag in the Ban Qing granules TLC identification test was due to the Maillard reaction between arginine and the sugar reduced from sucrose during the TLC sample preparation process, and this could be eliminated by modifying TLC sample preparation process:replacing the homeopathic alcohol with 95% alcohol, and replacing the steam drying by natural volatilization drying. These results indicated that, the Maillard reaction between arginine and the reduced sugar from the sucrose in the Ban Qing granules during the TLC sample preparation, is the key reason for the arginine spots lag in TLC test. Modification of TLC sample preparation could eliminate the effect of sucrose on the TLC identification of Ban Qing granules and resolve the spot lag problem. This provides a necessary scientific basis to revise the quality standard of Ban Qing granules.

Key words: Ban Qing granules, thin layer chromatography identification method, spot lag, method improved, sucrose, Maillard reaction

中图分类号:

R927.1

杨新, 李硕, 李亚威, 陈薇, 范琦, 郑春红, 高经纬, 黄琦, 周锐, 刘洁. 蔗糖对板青颗粒薄层色谱鉴别影响的发现和消除[J]. 畜牧兽医学报, 2019, 50(8): 1702-1708.

YANG Xin, LI Shuo, LI Yawei, CHEN Wei, FAN Qi, ZHENG Chunhong, GAO Jingwei, HUANG Qi, ZHOU Rui, LIU Jie. Characterization and Elimination of the Effect of Sucrose on Ban Qing Granules TLC Identification[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1702-1708.

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蔗糖对板青颗粒薄层色谱鉴别影响的发现和消除

Characterization and Elimination of the Effect of Sucrose on Ban Qing Granules TLC Identification

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