细菌脂多糖急性暴露通过炎症反应诱导小鼠睾丸损伤

doi:10.11843/j.issn.0366-6964.2019.05.021

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (5): 1099-1105.doi: 10.11843/j.issn.0366-6964.2019.05.021

细菌脂多糖急性暴露通过炎症反应诱导小鼠睾丸损伤

张逢^1,2, 陈指龙¹, 易思亮¹, 许璐瑶¹, 方娟¹, 黎陈¹, 傅胜才^2*, 杨青^1*

1. 湖南农业大学动物医学院, 长沙 410128;
2. 湖南省畜牧兽医研究所, 长沙 410131

收稿日期:2018-12-07 出版日期:2019-05-23 发布日期:2019-05-23
通讯作者: 傅胜才,主要从事动物疫病防控研究,E-mail:shcamail@sina.com;杨青,主要从事动物生殖调控研究,E-mail:qingyanghn@hunau.edu.cn
作者简介:张逢(1992-),女,湖南怀化人,硕士生,主要从事动物疫病防控研究,E-mail:1161796800@qq.com
基金资助:
国家自然科学基金（31572591；31772819）；国家级大学生创新创业训练计划项目（（G）SCX1817）

Acute Exposure of Bacterial Lipopolysaccharide Induces Testicular Injury in Mice through Inflammatory Response

ZHANG Feng^1,2, CHEN Zhilong¹, YI Siliang¹, XU Luyao¹, FANG Juan¹, LI Chen¹, FU Shengcai^2*, YANG Qing^1*

1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Institute of Animal Husbandry and Veterinary Medicine, Changsha 410131, China

Received:2018-12-07 Online:2019-05-23 Published:2019-05-23

摘要/Abstract

摘要：

旨在研究细菌脂多糖（LPS）急性暴露对小鼠睾丸及其功能的影响。将健康雄性小鼠随机分为4组：对照组（Control）、1.25 mg·kg^-1 LPS组、2.5 mg·kg^-1 LPS组和5.0 mg·kg^-1 LPS组，对照组腹腔注射200 μL生理盐水，其余3组注射等体积含不同剂量的LPS，作用12 h。收集睾丸组织制作石蜡切片，染色后观察其病理变化，记录体重、睾丸和附睾指数，并测定精子品质相关指标，用RT-qPCR法检测炎症相关基因TNF-α、IL-6、GAS6和TGF-β1的转录，同时用ELISA检测血清中睾酮（T）的含量。结果表明，与对照组相比，LPS急性暴露时，以剂量依赖性方式降低小鼠体重（P<0.05或P<0.01），同时显著降低睾丸指数（P<0.05或P<0.01）、精子密度（P<0.01）及精子活力（P<0.05或P<0.01），增加精子畸形率（P<0.05或P<0.01）；使睾丸组织发生不同程度的病理学变化，表现在生精细胞凋亡，曲细精管管腔增大，精子数量减少等；导致血清中睾酮（T）的含量显著下降；另外，LPS还呈剂量依赖方式增加促炎因子TNF-α和IL-6及抑制抗炎因子GAS6和TGF-β1的转录。结果提示，细菌LPS急性暴露可通过破坏机体中炎症因子的平衡，使睾丸组织受到损伤从而导致生精障碍。

关键词: 脂多糖, 睾丸, 炎症因子, 睾酮

Abstract:

The present study was conducted to investigate the effects of acute exposure to bacterial lipopolysaccharide (LPS) on testis and its function in mice. Healthy male mice were randomly divided into 4 groups:Control group, 1.25 mg·kg^-1 LPS group, 2.5 mg·kg^-1 LPS group and 5.0 mg·kg^-1 LPS. The control group was intraperitoneally injected with 200 μL saline, while the other three groups were injected the same volume saline containing different doses of LPS and treated for 12 hours. Testes were collected to check the pathological changes after staining; body weight, testicular and epididymal indexes were recorded and the related sperm quality indexes were determined; testosterone (T) level in serum was measured by ELISA; Transcription of inflammatory genes (TNF-α, IL-6, GAS6 and TGF-β1) were detected by RT-qPCR. The results showed that LPS acute exposure reduced the body weight of mice in a dose-dependent manner (P<0.05 or P<0.01), significantly decreased testicular index (P<0.05 or P<0.01), sperm density (P<0.01) and sperm motility (P<0.05 or P<0.01), and increased the rate of sperm malformation (P<0.05 or P<0.01). Meanwhile, LPS exposure resulted in testicular pathological changes to varying degrees, such as spermatogenic cell apoptosis, enlargement of seminiferous duct cavity and less sperms, and significantly reduced the levels of testosterone (T) in serum. In addition, LPS also increased the transcription of pro-inflammatory factors (TNF-α and IL-6) and inhibited anti-inflammatory factors (GAS6 and TGF-β1) in a dose-dependent manner. These results indicated that acute exposure to bacterial LPS impairs testicular tissue and leads to spermatogenesis disorder via destroying the balance of inflammatory factors in the body.

Key words: lipopolysaccharide, testis, inflammatory factor, testosterone

中图分类号:

S857.29

张逢, 陈指龙, 易思亮, 许璐瑶, 方娟, 黎陈, 傅胜才, 杨青. 细菌脂多糖急性暴露通过炎症反应诱导小鼠睾丸损伤[J]. 畜牧兽医学报, 2019, 50(5): 1099-1105.

ZHANG Feng, CHEN Zhilong, YI Siliang, XU Luyao, FANG Juan, LI Chen, FU Shengcai, YANG Qing. Acute Exposure of Bacterial Lipopolysaccharide Induces Testicular Injury in Mice through Inflammatory Response[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1099-1105.

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细菌脂多糖急性暴露通过炎症反应诱导小鼠睾丸损伤

Acute Exposure of Bacterial Lipopolysaccharide Induces Testicular Injury in Mice through Inflammatory Response

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