饲料磷真消化率不同的山羊瘤胃微生物差异性研究

doi:10.11843/j.issn.0366-6964.2018.12.014

摘要/Abstract

摘要：

旨在通过高通量测序技术比较饲料磷真消化率不同的山羊瘤胃微生物结构与组成的差异。本研究选取28只10月龄，雌性，平均体重（24.25±2.47）kg的努比亚黑山羊作为试验动物。通过两次消化试验，然后测定山羊饲料和粪便样品中的磷含量，采用差量法计算所有山羊饲料磷的真消化率。从所有山羊中筛选出高（HP）和低（LP）饲料磷真消化率组，每组各6只。分别采集HP、LP组每只山羊瘤胃内容物，提取微生物总DNA，用细菌通用引物，针对细菌16S rRNA基因的高可变区进行PCR扩增，利用Illumina MiSeq平台对扩增子进行高通量测序，并用QⅡME等软件对测序序列进行生物信息学分析。结果表明：1）HP组山羊血液中磷的含量显著高于LP组（P<0.05）。2）在门水平上，厚壁菌门和拟杆菌门在两组山羊瘤胃微生物中均为优势菌门。3）在属水平，有10个菌属的相对丰度在组间具有显著差异；其中，HP组有3个菌属的相对丰度显著高于LP组（P<0.05），分别为Prevotella、Desulfovibrio和Selenomonas，其它7个菌属（Ruminococcaceae_UCG-010、Ruminococcus_2、Ruminococcaceae_NK4A214_group、Saccharofermentans、Ruminococcaceae_UCG-014、Butyrivibrio和Clostridium）的相对丰度均显著低于LP组（P<0.05）。瘤胃微生物的相对丰度与山羊饲料磷的真消化率显著相关。不同饲料磷真消化率山羊的瘤胃微生物结构与组成存在显著性差异。

关键词: 山羊, 饲料磷真消化率, 瘤胃, 微生物

Abstract:

The objective of this study was to compare the structure and composition of rumen microorganism of goats with different true digestibility of feed phosphorus using Illumina MiSeq sequencing technology. Twenty-eight Nubian Black goats, aged 10 months on average and weighed (24.25±2.47) kg, were selected as the experimental animals. After two digestion tests, the content of phosphorus in feed and feces was determined, and the true digestibility of phosphorus in all goat feed was calculated by differential method. Among them, 6 goats with high phosphorus true digestibility were selected as HP group and 6 goats with low phosphorus true digestibility as LP group. Rumen fluid from each goat in HP and LP groups was collected and then total genomic DNA was extracted. High variable region of bacterial 16S rRNA gene was amplified by PCR using bacterial universal primers. The amplicon sequence data was analyzed by the Illumina MiSeq sequencing platform, and bioinformatics analysis was performed using QⅡME software. The results showed that:1) The content of phosphorus in blood of goats in HP group was significantly higher than that in LP group (P<0.05). 2) At the phylum level, Firmicutes and Bacteroidetes were the dominant bacteria in the rumen microorganisms of goats in the two groups; 3)At the genus level, the relative abundances of 10 genus were significantly different between the two groups (P<0.05). Only the relative abundances of Prevotella, Desulfovibrio and Selenomonas in HP group were significantly higher than that in LP group(P<0.05), but the relative abundances of Ruminococcaceae_UCG-010, Ruminococcus_2, Ruminococcaceae_NK4A214_group, Saccharofermentans, Ruminococcaceae_UCG-014, Butyrivibrio and Clostridium were significantly lower in HP group than that in LP group (P<0.05). The relative abundance of rumen microorganisms was significantly correlated with the phosphorus true digestibility in goats. There are significant differences in structure and composition of rumens microorganisms among goats with different phosphorus true digestibility.

Key words: goat, true digestibility of feed phosphorus, rumen, microorganism

中图分类号:

S827.5

金磊, 王立志, 谷可, 王之盛, 薛白, 彭全辉. 饲料磷真消化率不同的山羊瘤胃微生物差异性研究[J]. 畜牧兽医学报, 2018, 49(12): 2652-2663.

JIN Lei, WANG Li-zhi, GU Ke, WANG Zhi-sheng, XUE Bai, PENG Quan-hui. Differences in Rumen Microbes of Goats with Different Phosphorus True Digestibility[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2652-2663.

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