ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位

doi:10.11843/j.issn.0366-6964.2018.06.024

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (6): 1307-1313.doi: 10.11843/j.issn.0366-6964.2018.06.024

ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位

高建锋^1,2, 李讨讨^1,2, 陈灿灿^1,2, 赵兴绪³, 马友记^1,2*

1. 甘肃农业大学动物科学技术学院, 兰州 730070;
2. 甘肃省肉羊繁育生物技术工程实验室, 民勤 733300;
3. 甘肃农业大学动物医学院, 兰州 730070

收稿日期:2017-11-06 出版日期:2018-06-23 发布日期:2018-06-22
通讯作者: 马友记,教授,主要从事绵山羊繁育与疫病防治工作,E-mail:yjma@gsau.edu.cn
作者简介:高建锋(1991-),女,陕西榆林人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:1518867937@qq.com
基金资助:
甘肃省科技重大专项计划（1602NKDH020）；甘肃农业大学校级课题（GSAU-ZL-2015-030）；国家肉羊产业技术体系项目（CARS-38）

Expression and Localization of ANXA2 and ERp29 in Mammary Gland Tissue of Normal and Clinical Mastitis Sheep(Ovis aries)

GAO Jian-feng^1,2, LI Tao-tao^1,2, CHEN Can-can^1,2, ZHAO Xing-xu³, MA You-ji^1,2*

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Gansu Engineering Laboratory of Sheep Breeding Biotechnology, Minqin 733300, China;
3. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received:2017-11-06 Online:2018-06-23 Published:2018-06-22

摘要/Abstract

摘要：

旨在探讨膜联蛋白A2（annexin A2，ANXA2）与内质网蛋白29（endoplasmic reticulum protein 29，ERp29）基因在正常与临床型乳房炎绵羊（Ovis aries）乳腺组织中的表达与定位。选取正常与临床型乳房炎绵羊各3只，采集乳腺组织，通过qRT-PCR、免疫印迹（Western blot，WB）和免疫组织化学（immunohistochemistry，IHC）方法检测正常与临床型乳房炎绵羊乳腺组织中ANXA2与ERp29 mRNA和蛋白的表达与定位。结果表明，ANXA2在临床型乳房炎绵羊乳腺组织中mRNA的表达极显著高于正常型（P<0.01），而该蛋白的表达却极显著低于正常型（P<0.01）；ERp29在临床型乳房炎绵羊乳腺组织中mRNA与蛋白表达均极显著高于正常型（P<0.01）。ANXA2和ERp29分别在正常与临床型乳房炎绵羊乳腺组织中均有阳性反应，且主要定位于乳腺上皮细胞。综上表明，ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达均差异显著，其可能参与了绵羊乳房炎的发生与发展的调控。

关键词: 绵羊, 乳房炎, ANXA2, ERp29, 表达, 定位

Abstract:

The study aimed to investigate the expression and localization of annexin2 (ANXA2) and endoplasmic reticulum protein 29 (ERp29) genes in mammary gland tissue of normal and clinical mastitis sheep (Ovis aries). In this study, mammary gland tissues of 3 normal and 3 clinical mastitis sheep (Ovis aries) were sampled. qRT-PCR, Western blot (WB) and immunohistochemistry (IHC) were used to detect the expression and distribution of ANXA2 and ERp29 mRNA and proteins in mammary gland tissue of normal and clinical mastitis sheep.The results showed that the expression of ANXA2 mRNA in the mammary gland tissue of clinical mastitis sheep was significantly higher than that of normal group (P<0.01), while the expression of ANXA2 protein was significantly lower than that of normal group (P<0.01). The expression of ERp29 mRNA and protein in the mammary gland tissue of clinical mastitis sheep were significantly higher than that of normal group (P<0.01). ANXA2 and ERp29 proteins had positive reactions in mammary gland tissues of normal and clinical mastitis sheep respectively and mainly located in mammary epithelial cells. The expression of ANXA2 and ERp29 were significantly different in the mammary gland tissue of normal and clinical mastitis sheep, which may be involved in the regulation of the occurrence and development of sheep mastitis.

Key words: sheep, mastitis, ANXA2, ERp29, expression, location

中图分类号:

S826.2

高建锋, 李讨讨, 陈灿灿, 赵兴绪, 马友记. ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位[J]. 畜牧兽医学报, 2018, 49(6): 1307-1313.

GAO Jian-feng, LI Tao-tao, CHEN Can-can, ZHAO Xing-xu, MA You-ji. Expression and Localization of ANXA2 and ERp29 in Mammary Gland Tissue of Normal and Clinical Mastitis Sheep(Ovis aries)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1307-1313.

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ANXA2与ERp29在正常与临床型乳房炎绵羊乳腺组织中的表达与定位

Expression and Localization of ANXA2 and ERp29 in Mammary Gland Tissue of Normal and Clinical Mastitis Sheep(Ovis aries)

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