广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析

doi:10.11843/j.issn.0366-6964.2018.06.019

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (6): 1265-1273.doi: 10.11843/j.issn.0366-6964.2018.06.019

广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析

季程远, 王蔚怡, 冯选榕, 姚静, 黄欣, 冯园青, 覃一峰, 方庆励, 陈樱, 欧阳康, 韦祖樟, 黄伟坚*

广西大学动物科学技术学院, 南宁 530004

收稿日期:2017-12-06 出版日期:2018-06-23 发布日期:2018-06-22
通讯作者: 黄伟坚(1963-),E-mail:huangweijian-1@163.com
作者简介:季程远(1994-),男,江苏泰州人,硕士生,主要从事动物传染病学与分子免疫学研究,E-mail:710406303@qq.com
基金资助:
广西科技厅重点研发项目（桂科AB16380135）；广西创新驱动发展专项资金项目（桂科AA17204057-1）

Epidemiological Investigation and Genetic Diversity Analysis of Porcine Circovirus Type 3 in Slaughter Pigs of a City in Guangxi

JI Cheng-yuan, WANG Wei-yi, FENG Xuan-rong, YAO Jing, HUANG Xin, FENG Yuan-qing, QIN Yi-feng, FANG Qing-li, CHEN Ying, OUYANG Kang, WEI Zu-zhang, HUANG Wei-jian*

College of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received:2017-12-06 Online:2018-06-23 Published:2018-06-22

摘要/Abstract

摘要：

为了解猪圆环病毒3型（PCV3）在广西地区健康猪群中的遗传变异情况，对广西某市屠宰场健康猪的181份淋巴结进行PCV3的病原检测，结果PCV3阳性率为24.31%。这说明在健康屠宰猪群中PCV3的感染率较高，带毒情况较为严重。对其中的阳性样品进行全序列扩增，最终得到6条PCV3全基因组序列。将试验所得PCV3全基因组序列与GenBank上已发表的国内外毒株全基因组序列相比较，结果发现PCV3全基因组相似性为98.6%~99.9%，变异程度低，较为保守；对PCV3 ORF2基因进行遗传演化分析，发现PCV3毒株可分为两大型。通过对PCVs（PCV1、PCV2和PCV3） Cap蛋白相似性进行比对分析，推测PCV2与PCV3不具有交叉保护性。

关键词: 猪圆环病毒3型, 全基因, 序列分析, 遗传演化分析

Abstract:

In order to understand the epidemic status and phylogenetic relationship of porcine circovirus type 3 in healthy slaughter pigs, 181 lymph nodes collected from slaughterhouse in Guangxi were detected for PCV3 pathogens. PCV3 infection in healthy slaughter pigs was detected by PCR method. The positive rate of PCV3 was 24.31%, which suggest that the infection of PCV3 in healthy pigs is more severe. Six PCV3 complete genome sequences were amplified with PCR in the positive samples.Compared with PCV3 complete genome in GenBank and the results showed the nucleotide homology of PCV3 sequences was 98.6%-99.9% and genes of PCV3 were very conservative and similar. Genetic evolution analysis of PCV3 ORF2 gene showed that PCV3 strains could be divided into two major types. Analyses of the phylogenetic relationship of PCVs Cap and suggesting cross protection between PCV2 and PCV3 is impossible.

Key words: porcine circovirus type 3, complete genenome, sequence analysis, phylogenetic analysis

中图分类号:

S852.659.2

季程远, 王蔚怡, 冯选榕, 姚静, 黄欣, 冯园青, 覃一峰, 方庆励, 陈樱, 欧阳康, 韦祖樟, 黄伟坚. 广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析[J]. 畜牧兽医学报, 2018, 49(6): 1265-1273.

JI Cheng-yuan, WANG Wei-yi, FENG Xuan-rong, YAO Jing, HUANG Xin, FENG Yuan-qing, QIN Yi-feng, FANG Qing-li, CHEN Ying, OUYANG Kang, WEI Zu-zhang, HUANG Wei-jian. Epidemiological Investigation and Genetic Diversity Analysis of Porcine Circovirus Type 3 in Slaughter Pigs of a City in Guangxi[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1265-1273.

参考文献

[1] TISCHER I, GELDERBLOM H, VETTERMANN W, et al. A very small porcine virus with circular single-stranded DNA[J]. Nature, 1982,295(5844):64-66.
[2] FRANZO G,TUCCIARONE C M,DOTTO G, et al. International trades, local spread and viral evolution:The case of porcine circovirus type 2(PCV2) strains heterogeneity in Italy[J]. Infect Genet Evol, 2015, 32:409-415.
[3] MOROZOV I, SIRINARUMITR T, SORDEN S D, et al. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome[J].J Clin Microbiol, 1998, 36(9):2535-2541.
[4] MAHÉ D, BLANCHARD P, TRUONG C, et al. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes[J]. J Gen Virol, 2000, 81:1815-1824.
[5] LIU Q, WILLSON P, ATTOH-POKU S, et al. Bacterial expression of an immunologically reactive PCV2 ORF2 fusion protein[J].Protein Expr Purif, 2001, 21(1):115-120.
[6] NAWAGITGUL P, MOROZOV I, BOLIN S R, et al. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein[J]. J Gen Virol, 2000, 81(Pt 9):2281-2287.
[7] TISCHER I, RASCH R, TOCHTERMANN G. Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines[J]. Zentralbl Bakteriol Orig A, 1974, 226(2):153-167.
[8] HARMS P A, SORDEN S D, HALBUR P G, et al. Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus[J].Vet Pathol, 2001, 38(5):528-539.
[9] CHOI C, KIM J, KANG I J, et al. Concurrent outbreak of PMWS and PDNS in a herd of pigs in Korea[J]. Vet Rec, 2002, 151(16):484-485.
[10] HINES R K, LUKERT P D. Porcine circovirus as a cause of congenital tremors in newborn pigs[C]//Proceedings of America Association Swine Prsctitioners. Chicago:America Association Swine Prsctitioners,1994:344-345.
[11] PALINSKI R, PIÑEYRO P, SHANG P C, et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure[J]. J Virol, 2016, 91(1):e01879-16.
[12] GRAU-ROMA L, FRAILE L, SEGALÉS J. Recent advances in the epidemiology,diagnosis and control of diseases caused by porcine circovirus type 2[J].Vet J, 2011, 187(1):23-32.
[13] KU X,CHEN F,LI P,et al.Identification and genetic characterization of porcine circovirus type 3 in China[J]. Transbound Emerg Dis, 2017, 64(3):703-708.
[14] CHEN G H, MAI K J, ZHOU L, et al. Detection and genome sequencing of porcine circovirus 3 in neonatal pigs with congenital tremors in South China[J].Transbound Emerg Dis, 2017, 64(6):1650-1654.
[15] 贺会利, 李军, 潘艳, 等. 广西首例猪圆环病毒3型的发现及其衣壳蛋白序列分析[J].南方农业学报, 2017, 48(8):1499-1503.
HE H L, LI J, PAN Y, et al. The first report of porcine circovirus type 3 infection in Guangxi and sequence analysis of its capsid protein[J].Journal of Southern Agriculture, 2017, 48(8):1499-1503. (in Chinese)

广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析

Epidemiological Investigation and Genetic Diversity Analysis of Porcine Circovirus Type 3 in Slaughter Pigs of a City in Guangxi

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	邱静静, 丁轲, 余祖华, 贾艳艳, 张春杰, 汪洋, 赵战勤. 2014-2017年河南省副猪嗜血杆菌分离菌株的PCR鉴定及分型[J]. 畜牧兽医学报, 2019, 50(8): 1649-1657.
[2]	牛凯, 程汝佳, 许冠龙, 冯宇, 孙石静, 范学政, 彭小薇, 朱良全, 秦玉明, 丁家波, 常维山, 蒋卉. 一株鹿源牛种布鲁菌的鉴定及全基因组分析[J]. 畜牧兽医学报, 2019, 50(8): 1666-1675.
[3]	邱静静, 丁轲, 余祖华, 贾艳艳, 张春杰, 汪洋, 赵战勤. 2014-2017年河南省副猪嗜血杆菌分离菌株的PCR鉴定及分型[J]. 畜牧兽医学报, 2019, 50(8): 1649-1657.
[4]	牛凯, 程汝佳, 许冠龙, 冯宇, 孙石静, 范学政, 彭小薇, 朱良全, 秦玉明, 丁家波, 常维山, 蒋卉. 一株鹿源牛种布鲁菌的鉴定及全基因组分析[J]. 畜牧兽医学报, 2019, 50(8): 1666-1675.
[5]	赵亚涵, 郝海生, 杜卫华, 庞云渭, 闫长亮, 刘岩, 赵善江, 赵学明, 朱化彬. 新鲜、玻璃化冷冻牛卵母细胞体外受精囊胚全基因组甲基化模式初探[J]. 畜牧兽医学报, 2019, 50(6): 1179-1188.
[6]	申松玮, 王泽源, 范威峰, 祝常椿, 陆游, 石宝兰, 刘雷, 沈海峰, 焦库华, 秦涛, 陈素娟, 彭大新, 刘秀梵. 2017-2018年华东地区H9亚型禽流感病毒分离毒株的抗原差异分析[J]. 畜牧兽医学报, 2019, 50(6): 1230-1238.
[7]	赵亚涵, 郝海生, 杜卫华, 庞云渭, 闫长亮, 刘岩, 赵善江, 赵学明, 朱化彬. 新鲜、玻璃化冷冻牛卵母细胞体外受精囊胚全基因组甲基化模式初探[J]. 畜牧兽医学报, 2019, 50(6): 1179-1188.
[8]	申松玮, 王泽源, 范威峰, 祝常椿, 陆游, 石宝兰, 刘雷, 沈海峰, 焦库华, 秦涛, 陈素娟, 彭大新, 刘秀梵. 2017-2018年华东地区H9亚型禽流感病毒分离毒株的抗原差异分析[J]. 畜牧兽医学报, 2019, 50(6): 1230-1238.
[9]	刘琪, 史利军, 梁琳, 张玲玲, 袁维峰, 梁瑞英, 李金祥, 崔尚金. 一株猫泛白细胞减少症病毒的分离鉴定及其VP2和NS1基因的变异分析[J]. 畜牧兽医学报, 2019, 50(5): 1106-1112.
[10]	刘琪, 史利军, 梁琳, 张玲玲, 袁维峰, 梁瑞英, 李金祥, 崔尚金. 一株猫泛白细胞减少症病毒的分离鉴定及其VP2和NS1基因的变异分析[J]. 畜牧兽医学报, 2019, 50(5): 1106-1112.
[11]	杨振兴, 孟锦昕, 肖雷, 朱建波, 廖德芳, 高林, 李占鸿, 杨恒, 李华春. 流行性出血病病毒(EHDV)血清7型毒株在中国的首次分离与鉴定[J]. 畜牧兽医学报, 2019, 50(3): 602-610.
[12]	杨振兴, 孟锦昕, 肖雷, 朱建波, 廖德芳, 高林, 李占鸿, 杨恒, 李华春. 流行性出血病病毒(EHDV)血清7型毒株在中国的首次分离与鉴定[J]. 畜牧兽医学报, 2019, 50(3): 602-610.
[13]	李占鸿, 王金萍, 杨恒, 廖德芳, 宋建领, 高林, 何于雯, 李华春. 蓝舌病病毒血清9型毒株在我国的首次分离[J]. 畜牧兽医学报, 2019, 50(2): 354-363.
[14]	尹立林, 马云龙, 项韬, 朱猛进, 余梅, 李新云, 刘小磊, 赵书红. 全基因组选择模型研究进展及展望[J]. 畜牧兽医学报, 2019, 50(2): 233-242.
[15]	王俊伟, 陈芳洲, 库旭钢, 李畅, 何启盖. 基于PCV3-Cap蛋白间接ELISA检测方法的建立及临床应用[J]. 畜牧兽医学报, 2019, 50(2): 454-460.