猪繁殖与呼吸综合征病毒类NADC30毒株FJZ03的致病性分析

doi:10.11843/j.issn.0366-6964.2018.06.016

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (6): 1241-1248.doi: 10.11843/j.issn.0366-6964.2018.06.016

猪繁殖与呼吸综合征病毒类NADC30毒株FJZ03的致病性分析

刘建奎*, 范佳霖, 魏春华, 李艳, 刘佳悦, 马露露, 虞慧芸, 戴爱玲, 杨小燕*

龙岩学院生命科学学院, 福建省家畜传染病防治与生物技术重点实验室福建省生猪疫病防控工程技术研究中心, 龙岩 364000

收稿日期:2017-09-19 出版日期:2018-06-23 发布日期:2018-06-22
通讯作者: 刘建奎,E-mail:liujiankui99@126.com;杨小燕,E-mail:lyyxy1988@126.com
作者简介:刘建奎(1979-),男,博士,山东聊城人,主要从事分子病原学研究
基金资助:
福建省科技重大专项（2014NZ0002-3）；福建省自然科学基金（2016J01168）；福建省大学生创新创业计划训练项目（201711312043）

Pathogenicity of a NADC30-like Strain FJZ03 of Porcine Reproductive and Respiratory Syndrome Virus Emerged in China

LIU Jian-kui*, FAN Jia-lin, WEI Chun-hua, LI Yan, LIU Jia-yue, MA Lu-lu, YU Hui-yun, DAI Ai-ling, YANG Xiao-yan*

Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Fujian Engineering Research Center for Swine Disease Control and Prevention, College of Life Sciences, Longyan University, Longyan 364000, China

Received:2017-09-19 Online:2018-06-23 Published:2018-06-22

摘要/Abstract

摘要：

为了解猪繁殖与呼吸综合征病毒新流行毒株——类NADC30毒株的生物学特性和致病特征，将类NADC30 PRRSV FJZ03毒株和HP-PRRSV FJLYDX毒株分别接种30日龄PRRSV和PCV2阴性仔猪，观察、记录和对比各试验组感染猪的临床症状、体温变化、体外排毒情况、病毒血症及组织病变等情况。试验结果显示，FJZ03株能在猪体内快速复制，引起仔猪较高滴度的病毒血症，体温升高和体外排毒时间均早于高致病性毒株FJLYDX，并且引起严重的间质性肺炎和脾萎缩。细胞因子检测结果表明FJZ03组仔猪细胞因子的浓度都有不同程度的升高，与FJLYDX组相比最明显的是产生较高水平的TNF-α和IL-10。综上表明FJZ03对仔猪具有较强的致病性。

关键词: 猪繁殖与呼吸综合征病毒, NADC30-like PRRSV, 致病性

Abstract:

The aim of this study was to explore the biological characteristics and pathogenic of a NADC30-like PRRSV. Thirty days old piglets confirmed to be free of PRRSV and PCV2 were inoculated with a NADC30-like PRRSV FJZ03 strain and a HP-PRRSV FJLYDX strain, respectively. The clinical symptoms, rectal temperature, virus excretion, virus loads in the sera, and tissues lesions were monitored and recorded. We found that FJZ03 strain could be rapidly replicated and developed higher titer of viremia in piglets. Moreover, the time of rectal temperatures rose and virus excretion in FJZ03 infected piglets were earlier than that of FJLYDX infected. Furthermore, piglets infected with FJZ03 displayed severe interstitial pneumonia and spleen atrophy. Cytokine test results showed that the cytokines concentrations of sera of FJZ03 infected piglets increased at different degrees. The IL-10 and TNF-α concentration in FJZ03 group was obvious higher than that of the FJLYDX group. Therefore, our results indicated that FJZ03 is pathogenic for piglets.

Key words: porcine reproductive and respiratory syndrome virus, NADC30-like PRRSV, pathogenicity

中图分类号:

S852.659.6

刘建奎, 范佳霖, 魏春华, 李艳, 刘佳悦, 马露露, 虞慧芸, 戴爱玲, 杨小燕. 猪繁殖与呼吸综合征病毒类NADC30毒株FJZ03的致病性分析[J]. 畜牧兽医学报, 2018, 49(6): 1241-1248.

LIU Jian-kui, FAN Jia-lin, WEI Chun-hua, LI Yan, LIU Jia-yue, MA Lu-lu, YU Hui-yun, DAI Ai-ling, YANG Xiao-yan. Pathogenicity of a NADC30-like Strain FJZ03 of Porcine Reproductive and Respiratory Syndrome Virus Emerged in China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1241-1248.

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猪繁殖与呼吸综合征病毒类NADC30毒株FJZ03的致病性分析

Pathogenicity of a NADC30-like Strain FJZ03 of Porcine Reproductive and Respiratory Syndrome Virus Emerged in China

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