关键词: 水貂嵌杯病毒, 基因组, 分析, 高通量测序

Abstract:

To analyze the characterization of the mink calicivirus in China, the genome of the mink calicivirus detected in the farm of Qingdao was sequenced by high-throughput sequencing. The genome of the isolate was analyzed and compared with other caliciviruses. The results showed that the length of the isolate was 8 427 bp, which possessed the similar genomic organization, structure and gene order with the other calicivirus. It possessed 5'-untranslated region and 3'-poly (A) tail. The comparing analysis of the genome and capsid protein amino acid sequence of the virus and the 19 representative viruses showed that the identity between this virus and the Chinese virus detected in 2011 was high. The BLAST results showed that this virus was not belong to mink enteric calicivirus. The analysis was also useful to supply reference to the classification of the caliciviruses which haven't been defied into genera.

Key words: mink calicivirus, genome, analysis, high-throughput sequencing