饲粮铜水平对育成期雄性水貂铜元素代谢和血清生化指标的影响

doi:10.11843/j.issn.0366-6964.2018.04.011

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (4): 746-753.doi: 10.11843/j.issn.0366-6964.2018.04.011

饲粮铜水平对育成期雄性水貂铜元素代谢和血清生化指标的影响

吴学壮^1,2, 杨颖³, 刘汇涛³, 高秀华^2*, 杨福合³, 戴四发¹, 邢秀梅³

1. 安徽科技学院动物科学学院, 蚌埠 233100;
2. 中国农业科学院饲料研究所, 农业部饲料生物技术重点实验室, 北京 100081;
3. 中国农业科学院特产研究所, 长春 130112

收稿日期:2017-10-16 出版日期:2018-04-23 发布日期:2018-04-19
通讯作者: 高秀华,研究员,博士生导师,E-mail:xiuhuagao@126.com
作者简介:吴学壮(1985-),男,山东聊城人,博士,主要从事新型饲料资源及动物营养调控研究,E-mail:wuxuezhuang@126.com。
基金资助:
国家自然资源平台专项“特种经济动物种质资源共享平台”（2005DKA21102）；安徽省自然科学基金（1708085QC74）；安徽科技学院高层次人才引进项目（DKYJ201701）；农业部饲料生物技术重点实验室开放课题资助；安徽科技学院重点学科建设项目（AKZDXK2015B03）

Effects of Dietary Copper Levels on Copper Metabolism and Serum Biochemical Indices in Late Growing Male Mink

WU Xue-zhuang^1,2, YANG Ying³, LIU Hui-tao³, GAO Xiu-hua^2*, YANG Fu-he³, DAI Si-fa¹, XING Xiu-mei³

1. College of Animal Science, Anhui Science and Technology University, Bengbu 233100, China;
2. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2017-10-16 Online:2018-04-23 Published:2018-04-19

摘要/Abstract

摘要：

旨在探讨饲粮不同铜水平对雄性水貂铜代谢、血清微量元素含量、血脂和血清抗氧化指标的影响。本研究选取（60±3）日龄的健康雌性水貂80只，随机分为8个组，每组10个重复，分别饲喂在基础日粮中添加0（Control组）、4（Cu4组）、8（Cu8组）、16（Cu16组）、32（Cu32组）、64（Cu64组）、128（Cu128组）和256 mg·kg^-1（Cu256组）铜的试验饲粮。试验预试期7 d，正试期45 d。正试期开始第30天，每组选择8只水貂进行铜元素代谢指标测定；饲养试验结束后，采集血液样品进行血清生化指标测定。结果表明：1）水貂摄入铜、粪铜、尿铜和铜在体内的沉积量随日粮铜水平增加呈一次线性和二次曲线极显著增加（linear，quadratic，P<0.01）。2）水貂血清铜含量随日粮铜水平的增加而升高（linear，quadratic，P<0.01）。3）水貂血清总胆固醇（TC，linear，quadratic，P<0.01）和甘油三酯（TG，linear，P<0.05；quadratic，P<0.01）含量随日粮铜水平的增加呈线性和二次曲线降低。4）水貂血清铜蓝蛋白（CER）和铜锌超氧化物歧化酶（Cu-Zn SOD）活性随日粮铜水平的增加呈二次曲线先增加后降低（quadratic，P<0.01）。综合以上指标可见，水貂日粮添加铜可以增加体内铜的沉积量和血清铜含量，同时能够降低血清胆固醇和甘油三酯水平，提高抗氧化酶活性。

关键词: 水貂, 铜, 血清生化指标, 脂类代谢

Abstract:

This experiment was conducted to study the effects of dietary copper levels on copper balance, serum trace element contents, serum lipid metabolism parameters and serum antioxidant indices in male mink. Eighty healthy (60±3) day-old male minks were randomly divided into 8 groups with 10 minks in each group. 0 (Control), 4 (Cu4), 8 (Cu8), 16 (Cu16), 32 (Cu32), 64 (Cu64), 128 (Cu128)and 256 mg·kg^-1 (Cu256)of copper levels were added in basic diet, respectively. The pre-test period lasted for 7 days, and the trial lasted for 45 days. On day 30 of the trail, eight animals from each treatment group were selected randomly to determine copper balance. Blood samples were collected from mink before sacrifice to measure the serum biochemical indices. The results showed that:1) Intake of copper, fecal copper, urinary copper, retention copper had a linear and quadratic (P<0.01) increase with the increasing dietary copper level. 2) There was a linear and quadratic (P<0.01) effect of dietary copper level on plasma Cu concentrations. 3) Serum TC (linear, quadratic, P<0.01), TG (linear, P<0.05;quadratic, P<0.01) contents were decreased with the increasing dietary copper level. 4)There was a quadratic (P<0.01) effect of dietary copper level on plasma ceruloplasmin concentration and Cu-Zn superoxide dismutase activity. These results indicate that supplementing Cu in the diet can increase Cu retention and plasma Cu concentrations, but reduce serum TC and TG levels, and improve antioxidant enzyme activity.

Key words: mink, copper, serum biochemical indices, lipid metabolism

中图分类号:

S829.95

吴学壮, 杨颖, 刘汇涛, 高秀华, 杨福合, 戴四发, 邢秀梅. 饲粮铜水平对育成期雄性水貂铜元素代谢和血清生化指标的影响[J]. 畜牧兽医学报, 2018, 49(4): 746-753.

WU Xue-zhuang, YANG Ying, LIU Hui-tao, GAO Xiu-hua, YANG Fu-he, DAI Si-fa, XING Xiu-mei. Effects of Dietary Copper Levels on Copper Metabolism and Serum Biochemical Indices in Late Growing Male Mink[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 746-753.

参考文献

[1] 李敏, 崔伟, 彭西, 等. 高铜对雏鸡脑组织抗氧化酶活性的影响[J]. 畜牧兽医学报, 2010, 41(2):220-223.
LI M, CUI W, PENG X, et al. Effect of dietary high copper on the antioxidase activities of brain tissue in chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2010, 41(2):220-223. (in Chinese)
[2] 崔伟, 李敏, 彭西, 等. 高铜对雏鸡肾脏组织结构及生化指标影响的研究[J]. 畜牧兽医学报, 2010, 41(1):86-91.
CUI W, LI M, PENG X, et al. Effect of dietary high copper on tissue structure and biochemical parameters of kidney in chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2010, 41(1):86-91. (in Chinese)
[3] 刘好朋, 唐兆新, 苏荣胜, 等. 高铜日粮对肉鸡肝脏TrxR2基因mRNA表达和还原活性的影响[J]. 畜牧兽医学报, 2011, 42(3):423-428.
LIU H P, TANG Z X, SU R S, et al. Effect of high level copper diet on expression of trxR2 mRNA and reduction activity of trxR2 of liver in broiler[J]. Acta Veterinaria et Zootechnica Sinica, 2011, 42(3):423-428. (in Chinese)
[4] WU X Z, LIU Z, GUO J G, et al. Influence of dietary zinc and copper on apparent mineral retention and serum biochemical indicators in young male mink (Mustela vison)[J]. Biol Trace Elem Res, 2015, 165(1):59-66.
[5] HOSIENPOUR N, NOROUZIAN M A, AFZALZADEH A, et al. Source of copper may have regressive effects on serum cholesterol and urea nitrogen among male fattening lambs[J]. Biol Trace Elem Res, 2014, 159(1-3):147-151.
[6] LIU L, SUI X Y, LI F C. Effect of dietary copper addition on lipid metabolism in rabbits[J]. Food Nutr Res, 2017, 61(1):1348866.
[7] NETTO A S, ZANETTI M A, DEL CLARO G R, et al. Effects of copper and selenium supplementation on performance and lipid metabolism in confined brangus bulls[J]. Asian-Aust J Anim Sci, 2014, 27(4):488-494.
[8] LEOSCHKE W L. Nutrition and nutritional physiology of the mink:a historical perspective[M]. Victoria, Canada:Trafford Publishing, 2011.
[9] 杨凤. 动物营养学[M]. 北京:中国农业出版社, 2010.
YANG F. Animal nutrition[M]. Beijing:China Agriculture Press, 2010. (in Chinese)
[10] RAMOS D, MAR D, ISHIDA M, et al. Mechanism of copper uptake from blood plasma ceruloplasmin by mammalian cells[J]. PLoS One, 2016, 11(3):e0149516.
[11] SQUITTI R, GHIDONI R, SIMONELLI I, et al. Copper dyshomeostasis in Wilson disease and Alzheimer's disease as shown by serum and urine copper indicators[J]. J Trace Elem Med Biol, 2018, 45:181-188.
[12] GRAY L W, PENG F Y, MOLLOY S A, et al. Urinary copper elevation in a mouse model of Wilson's disease is a regulated process to specifically decrease the hepatic copper load[J]. PLoS One, 2012, 7(6):e38327.
[13] MEJBORN H. Effect of copper addition to mink feed during the growth and moulting period on growth, skin production, and copper retention[J]. Scientifur, 1989, 13(3):229-234.
[14] 马德磊. 日粮铜水平对生长肉兔生产性能、消化代谢、血液生化指标和MT-Ⅰ mRNA表达量的影响[D]. 泰安:山东农业大学, 2009.
MA D L. Effect of dietary copper supplement on growth performance, digestion, blood biochemical parameters and liver MT-I mRNA expression of growing meat rabbits[D]. Tai'an:Shandong Agricultural University, 2009. (in Chinese)
[15] VASHCHENKO G, MACGILLIVRAY R T A. Multi-copper oxidases and human iron metabolism[J]. Nutrients, 2013, 5(7):2289-2313.
[16] 刘志. 生长期蓝狐饲粮适宜铜水平和铜源的研究[D]. 北京:中国农业科学院, 2016.
LIU Z. Optimum copper levels and sources in growing and furring blue foxes[D]. Beijing:Chinese Academy of Agricultural Sciences, 2016. (in Chinese)
[17] 邢芳芳, 燕富永, 孔祥峰, 等. 甘氨酸铜、蛋氨酸铜替代硫酸铜对仔猪血清生化指标的影响[J]. 江苏农业学报, 2008, 24(3):378-380.
XING F F, YAN Y F, KONG X F, et al. Effects of Gly-Cu and Met-Cu replacing copper sulfate as dietary additive on serum biochemical parameters of piglets[J]. Jiangsu Journal of Agricultural Sciences, 2008, 24(3):378-380. (in Chinese)
[18] APGAR G A, KORNEGAY E T, LINDEMANN M D, et al. Evaluation of copper sulfate and a copper lysine complex as growth promoters for weanling swine[J]. J Anim Sci, 1995, 73(9):2640-2646.
[19] 徐晨晨, 王宝维, 葛文华, 等. 饲粮中不同水平铜对5~16周龄五龙鹅脂类代谢、抗氧化能力与免疫功能的影响[J]. 动物营养学报, 2014, 26(4):908-917.
XU C C, WANG B W, GE W H, et al. Effects of dietary different copper levels on lipid metabolism, antioxidant ability and immune function of Wulong geese at the age of 5 to 16 weeks[J]. Chinese Journal of Animal Nutrition, 2014, 26(4):908-917. (in Chinese)
[20] 王宝维, 徐晨晨, 葛文华, 等. 铜对1~4周龄五龙鹅脂类代谢、抗氧化能力及免疫器官指数的影响[J]. 动物营养学报, 2014, 26(8):2093-2100.
WANG B W, MA C X, GE W H, et al. Effects of copper on lipid metabolism, antioxidant ability and immune organ indexes of Wulong geese aged from 1 to 4 weeks[J]. Chinese Journal of Animal Nutrition, 2014, 26(8):2093-2100. (in Chinese)
[21] 王荣梅, 曹华斌, 李和平, 等. 高铜对肉鸡肝线粒体膜通透性、脂类代谢及肝和肌肉铜含量的影响[J]. 中国兽医学报, 2010, 30(2):243-246.
WANG R M, CAO H B, LI H P, et al. Effects of high level copper on mitochondrial permeability of liver, lipid metabolism, liver and muscle copper content in broiler[J]. Chinese Journal of Veterinary Science, 2010, 30(2):243-246. (in Chinese)
[22] RANJI P, RAUTHAN M, PITOT C, et al. Loss of HMG-CoA reductase in C. elegans causes defects in protein prenylation and muscle mitochondria[J]. PLoS One, 2014, 9(6):e100033.
[23] KIM S, CHAO P Y, ALLEN K G. Inhibition of elevated hepatic glutathione abolishes copper deficiency cholesterolemia[J]. FASEB J, 1992, 6(7):2467-2471.
[24] XU Q, XUE C Y, ZHANG Y, et al. Medium-chain fatty acids enhanced the excretion of fecal cholesterol and cholic acid in C57BL/6J mice fed a cholesterol-rich diet[J]. Biosci Biotechnol Biochem, 2013, 77(7):1390-1396.
[25] TANG Z R, GASPERKOVA D, XU J, et al. Copper deficiency induces hepatic fatty acid synthase gene transcription in rats by increasing the nuclear content of mature sterol regulatory element binding protein 1[J]. J Nutr, 2000, 130(12):2915-2921.
[26] 隋慧. 铜对猪原代肝细胞含铜酶活性的影响[J]. 饲料研究, 2013(4):32-35.
SUI H. The effect of copper on copper enzyme activity of the pig liver cells[J]. Feed Research, 2013(4):32-35. (in Chinese)
[27] JAROSZ L S, MAREK A, GRDZKI Z, et al. The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens[J]. J Anim Physiol Anim Nutr, 2018, 102(1):e326-e336.
[28] WU X Z, ZHANG T T, GUO J G, et al. Copper bioavailability, blood parameters, and nutrient balance in mink[J]. J Anim Sci, 2015, 93(1):176-184.

饲粮铜水平对育成期雄性水貂铜元素代谢和血清生化指标的影响

Effects of Dietary Copper Levels on Copper Metabolism and Serum Biochemical Indices in Late Growing Male Mink

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	荣敏, 张然然, 涂剑峰, 王天娇, 孙丽敏, 杨颖, 徐佳萍, 邢秀梅*. 美国短毛黑水貂快速生长过程中转录组差异表达分析[J]. 畜牧兽医学报, 2019, 50(9): 1787-1801.
[2]	裴若男, 杨帆, 廖建昭, 马飞洋, 马欣艳, 林毓茵, 姚启发, 唐兆新, 梁昭平. 高铜日粮对肉鸡肾氧化损伤和Nrf2信号通路相关基因表达的影响[J]. 畜牧兽医学报, 2019, 50(9): 1912-1919.
[3]	司丙文, 徐文财, 郭江鹏, 崔凯, 王淑艳, 屠焰, 刁其玉. 杂交构树青贮对杜寒杂交肉羊生产性能、血清指标及背最长肌脂肪酸组成的影响[J]. 畜牧兽医学报, 2019, 50(7): 1424-1432.
[4]	司丙文, 徐文财, 郭江鹏, 崔凯, 王淑艳, 屠焰, 刁其玉. 杂交构树青贮对杜寒杂交肉羊生产性能、血清指标及背最长肌脂肪酸组成的影响[J]. 畜牧兽医学报, 2019, 50(7): 1424-1432.
[5]	李兰会, 杜小龙, 王麒, 葛琳涵, 张乐超, 李雪梅, 李祥龙. 水貂DCT基因5'UTR克隆分析与启动子活性探究[J]. 畜牧兽医学报, 2019, 50(3): 524-533.
[6]	李兰会, 杜小龙, 王麒, 葛琳涵, 张乐超, 李雪梅, 李祥龙. 水貂DCT基因5'UTR克隆分析与启动子活性探究[J]. 畜牧兽医学报, 2019, 50(3): 524-533.
[7]	蔡珺珺, 袁田青, 杨银萍, 高双, 宫兴文. 多靶点的抗菌先导化合物的虚拟筛选及活性研究[J]. 畜牧兽医学报, 2018, 49(7): 1500-1510.
[8]	蔡珺珺, 袁田青, 杨银萍, 高双, 宫兴文. 多靶点的抗菌先导化合物的虚拟筛选及活性研究[J]. 畜牧兽医学报, 2018, 49(7): 1500-1510.
[9]	董晓静, 崔凯, 马泽芳, 李建栋, 李迎梅. N-氨甲酰谷氨酸对育成期水貂生长性能、营养物质消化率、氮代谢及血清生化指标的影响[J]. 畜牧兽医学报, 2018, 49(6): 1194-1203.
[10]	董晓静, 崔凯, 马泽芳, 李建栋, 李迎梅. N-氨甲酰谷氨酸对育成期水貂生长性能、营养物质消化率、氮代谢及血清生化指标的影响[J]. 畜牧兽医学报, 2018, 49(6): 1194-1203.
[11]	王静, 张海华, 徐逸男, 李仁德, 张雪蕾, 蔡熙姮, 李光玉. 饲粮维生素D和钙水平对冬毛期短毛雄性黑水貂生产性能、血清生化指标和脏器指数的影响[J]. 畜牧兽医学报, 2018, 49(5): 986-995.
[12]	王静, 张海华, 徐逸男, 李仁德, 张雪蕾, 蔡熙姮, 李光玉. 饲粮维生素D和钙水平对冬毛期短毛雄性黑水貂生产性能、血清生化指标和脏器指数的影响[J]. 畜牧兽医学报, 2018, 49(5): 986-995.
[13]	王楷宬, 庄青叶, 邱源, 王素春, 侯广宇. 临床样本中水貂嵌杯病毒的全基因组分析[J]. 畜牧兽医学报, 2018, 49(4): 771-776.
[14]	吴学壮, 杨颖, 刘汇涛, 高秀华, 杨福合, 戴四发, 邢秀梅. 饲粮铜水平对育成期雄性水貂铜元素代谢和血清生化指标的影响[J]. 畜牧兽医学报, 2018, 49(4): 746-753.
[15]	王楷宬, 庄青叶, 邱源, 王素春, 侯广宇. 临床样本中水貂嵌杯病毒的全基因组分析[J]. 畜牧兽医学报, 2018, 49(4): 771-776.