河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析

doi:10.11843/j.issn.0366-6964.2018.03.017

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (3): 597-605.doi: 10.11843/j.issn.0366-6964.2018.03.017

河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析

张若曦¹, 张志², 顾文源¹, 刘天驹¹, 李翀¹, 王建昌³, 李彬⁴, 袁万哲⁵, 王玉清¹, 韩庆安^1*

1. 河北省动物疫病预防控制中心, 石家庄 050035;
2. 中国动物卫生与流行病学中心, 青岛 266032;
3. 河北出入境检验检疫局技术中心, 石家庄 050051;
4. 江苏省农业科学院兽医研究所, 南京 210014;
5. 河北农业大学动物医学院, 保定 071001

收稿日期:2017-07-21 出版日期:2018-03-23 发布日期:2018-03-13
通讯作者: 韩庆安,研究员,E-mail:hanqa1970@126.com
作者简介:张若曦(1987-),男,河北石家庄人,博士,主要从事动物疫病诊断及防治工作,E-mail:Zhangrx19@126.com,Tel:0311-66570278
基金资助:
河北省高层次人才资助项目（2012-28）；河北省畜牧兽医局科技项目（2015-1-01）；河北省自然科学基金青年项目（C2017325001）；河北省高校百名优秀创新人才支持计划（Ⅲ）（SLRC2017039）

Detection of Porcine Diarrhea Associated Virus and Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus in Hebei Province

ZHANG Ruo-xi¹, ZHANG Zhi², GU Wen-yuan¹, LIU Tian-ju¹, LI Chong¹, WANG Jian-chang³, LI Bin⁴, YUAN Wan-zhe⁵, WANG Yu-qing¹, HAN Qing-an^1*

1. Hebei Provincial Center for Animal Disease Control and Prevention, Shijiazhuang 050035, China;
2. China Animal Health and Epidemiology Center, Qingdao 266032, China;
3. Inspection and Quarantine Technical Center, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China;
4. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
5. College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China

Received:2017-07-21 Online:2018-03-23 Published:2018-03-13

摘要/Abstract

摘要：

为了解当前河北地区猪腹泻主要相关病毒流行情况，对2016年4月-2017年2月收集的河北地区腹泻样品，采用荧光定量PCR检测猪流行性腹泻病毒（PEDV）、猪传染性胃肠炎病毒、猪轮状病毒，并对扩增获得的9株PEDV S基因进行测序和序列分析。结果显示，全年腹泻病发生率为9.70%，在春冬季节多发。其中猪流行性腹泻病毒检出率最高，为13.89%，其在仔猪中检出率为19.28%，在育肥猪中检出率为17.24%。对PEDV S基因氨基酸序列分析显示，目前河北省PEDV流行毒株分布在G2群中的2个进化分支，部分毒株发生多处独特氨基酸突变。本研究表明，2016年河北地区PEDV对仔猪的感染情况有所缓解，而对育肥猪感染率较高，PEDV流行毒株S蛋白进化分支和突变较多提示PEDV流行毒株毒力和抗原活性可能发生改变。

关键词: 猪病毒性腹泻, 流行病学调查, 猪流行性腹泻病毒, 序列分析

Abstract:

To understand the epidemiology of the pigs diarrhea virus in Hebei province, a total of 1 855 clinical samples were collected from April, 2016 to February, 2017 and subjected to detection of porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PoRV) by real-time PCR. Spike (S) genes of nine amplified PEDV were sequenced and analyzed. The results showed that positive rate of diarrhea was 9.70% and highest in winter and spring seasons. The positive rates of PEDV of total diarrhea samples, piglets diarrhea samples and fattening pigs diarrhea samples were 13.89%, 19.28% and 17.24%, respectively. The phylogenetic analysis of PEDV S genes amino acid sequence showed that the detected 9 S genes from Hebei were distributed in two branches of group Ⅱ. The detail analysis showed several unique amino acid mutations including deletions and insertions in the 9 S genes. These results indicate PEDV infection has fell on piglets but rise on fattening pigs, and the genetic diversity and mutations of S genes were potentially cause alternations of pathogenesis and antigenicity of pandemic PEDV in Hebei province.

Key words: porcine viral diarrhea, epidemiological investigation, PEDV, sequence analysis

中图分类号:

S852.65

张若曦, 张志, 顾文源, 刘天驹, 李翀, 王建昌, 李彬, 袁万哲, 王玉清, 韩庆安. 河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析[J]. 畜牧兽医学报, 2018, 49(3): 597-605.

ZHANG Ruo-xi, ZHANG Zhi, GU Wen-yuan, LIU Tian-ju, LI Chong, WANG Jian-chang, LI Bin, YUAN Wan-zhe, WANG Yu-qing, HAN Qing-an. Detection of Porcine Diarrhea Associated Virus and Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus in Hebei Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 597-605.

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河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析

Detection of Porcine Diarrhea Associated Virus and Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus in Hebei Province

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