副猪嗜血杆菌TbpA对仔猪的免疫效力评价

doi:10.11843/j.issn.0366-6964.2018.03.016

摘要/Abstract

摘要：

本研究旨在了解副猪嗜血杆菌（HPS）转铁结合蛋白A（TbpA）对仔猪的免疫保护性，从而为HPS新型疫苗的研发奠定基础。采用HPS血清型13型灭活全菌体以及浓度分别为0.5、0.25 mg·4 mL^-1的重组TbpA，经3次间隔期均为14 d的免疫后，检测仔猪抗体（IgG）水平及细胞因子（IL-5、IL-10、IFN-γ、TNF-α）水平；以相同血清型菌株7 LD₅₀剂量，腹腔攻毒，检查病理学变化及其免疫保护率。结果显示：HPS的重组TbpA能诱导仔猪产生较高水平的特异性抗体IgG，并使细胞因子显著升高。重组TbpA和灭活全菌体在免疫后均能使仔猪获得免疫保护，其中0.5 mg·4 mL^-1TbpA和灭活全菌体的免疫保护率均为100%，组织切片病理变化与攻毒对照组之间差异明显。HPS的TbpA能激发仔猪的体液免疫和细胞免疫，产生较强的免疫保护力，可以作为一种新的疫苗候选抗原。

关键词: 副猪嗜血杆菌, 转铁结合蛋白A, 体液和细胞免疫, 免疫保护率, 仔猪

Abstract:

The aim of this study was to investigate the immune efficacy of the transferrin-binding protein A of HPS and to lay a foundation for research and development of new vaccine. The piglets were immunized with the inactivated whole cell vaccine from HPS serotype 13 and recombinant protein TbpA(0.5 and 0.25 mg·4 mL^-1). The immunization was conducted at week 0, 2, 4 and then challenged with HPSLJ3 (7 LD₅₀). Serum antibody (IgG) and cytokines (IL-5, IL-10, IFN-γ, TNF-α) levels were measured by ELISA and pathological examination was performed. Results were as follows:Immunization and challenge assay demonstrated the recombinant protein TbpA could induce high levels of antibody (IgG) and cytokines. The protective efficacy of the inactivated whole cell and TbpA (0.5 mg·4mL^-1) vaccines was 100%. There was a significant difference between the pathological changes and the control group. TbpA can stimulate the humoral and cellular immunity, and produce powerful immune protection. These results suggested that TbpA is a potential candidate for developing a novel HPS vaccine.

Key words: Haemophilus parasuis, transferring-binding protein A, humoral and cellular immunity, immunoprotection, piglets

中图分类号:

S855.12

姚焱彬, 陈章, 储霞飞, 魏建忠, 孙裴, 李郁. 副猪嗜血杆菌TbpA对仔猪的免疫效力评价[J]. 畜牧兽医学报, 2018, 49(3): 588-596.

YAO Yan-bin, CHEN Zhang, CHU Xia-fei, WEI Jian-zhong, SUN Pei, LI Yu. Immune Efficacy of Transferrin-binding Protein A of Haemophilus parasuis in Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 588-596.

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