绵羊FGF7基因组织表达及其多态性与产羔数之间的关系

doi:10.11843/j.issn.0366-6964.2018.03.008

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (3): 525-533.doi: 10.11843/j.issn.0366-6964.2018.03.008

绵羊FGF7基因组织表达及其多态性与产羔数之间的关系

周梅¹, 曹晓涵¹, 贺小云¹, 孙庆¹, 狄冉¹, 胡文萍¹, 王翔宇¹, 张效生², 张金龙², 刘秋月^1*, 储明星^1*

1. 中国农业科学院北京畜牧兽医研究所, 农业部动物遗传育种与繁殖重点实验室, 北京 100193;
2. 天津市畜牧兽医研究所, 天津 300381

收稿日期:2017-07-17 出版日期:2018-03-23 发布日期:2018-03-13
通讯作者: 刘秋月,博士,副研究员,E-mail:liuqiuyue@caas.cn;储明星,博士,研究员,博士生导师,主要从事羊优异繁殖性状的分子机理研究,E-mail:mxchu@263.net
作者简介:周梅(1988-),女,安徽肥东人,博士生,主要从事动物分子育种研究,E-mail:1229zhoumei@163.com
基金资助:
国家自然科学基金（31772580）；国家转基因科技重大专项（2016ZX08009-003-006；2016ZX08010-005-003）；国家肉羊产业技术体系专项（CARS-38）；中央级公益性科研院所基本科研业务费专项（Y2017JC24；2017ywf-zd-13；2013ywf-zd-1；2015ywf-zd-2；2015ywf-zd-8）；中国农业科学院科技创新工程（ASTIP-IAS13；CAAS-XTCX2016010-01-03；CAAS-XTCX2016010-03-03；CAAS-XTCX2016011-02-02）；宁夏农林科学院科技创新先导资金（DWJLC-2016001）；内蒙古自治区科技重大专项；农业科研杰出人才及其创新团队项目；国家万人计划科技创新领军人才项目；天津市科技计划项目（16ZXZYNC00050）

Tissue Expression and Polymorphism of Sheep FGF 7 Gene and Their Association with Litter Size

ZHOU Mei¹, CAO Xiao-han¹, HE Xiao-yun¹, SUN Qing¹, DI Ran¹, HU Wen-ping¹, WANG Xiang-yu¹, ZHANG Xiao-sheng², ZHANG Jin-long², LIU Qiu-yue^1*, CHU Ming-xing^1*

1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Tianjin Institute of Animal Sciences, Tianjin 300381, China

Received:2017-07-17 Online:2018-03-23 Published:2018-03-13

摘要/Abstract

摘要：

旨在探究绵羊FGF 7基因组织表达水平及其多态性与产羔数之间的关系。本研究利用半定量反转录-聚合酶链式反应（sqRT-PCR）和实时荧光定量PCR（qPCR）技术对绵羊FGF 7基因在多羔小尾寒羊和单羔苏尼特羊以及小尾寒羊FecB不同基因型（BB、B+、++）各组织中的表达水平进行研究，同时采用Sequenom MassARRAY^®SNP技术，对多羔绵羊品种（小尾寒羊380只）和单羔绵羊品种（滩羊、苏尼特羊、萨福克羊、杜泊羊和草原型藏羊共380只）FGF 7基因g.57842893C>T位点多态性进行检测，并与小尾寒羊产羔数进行关联分析。结果表明，FGF 7基因在单、多羔绵羊的心和肺中高表达，在其它各组织呈中等或低丰度表达；FGF 7基因在多羔小尾寒羊绝大部分组织中的表达量均高于单羔苏尼特羊，但差异均不显著（P>0.05），FGF 7在小尾寒羊FecB不同基因型中的表达并无明显差异。g.57842893C>T位点共存在CC、CT和TT 3种基因型，且基因型频率和等位基因频率在单、多羔品种间差异均达到显著水平（P ≤ 0.05）；g.57842893C>T位点在苏尼特羊、萨福克羊和杜泊羊中表现为中度多态（0.25 < PIC < 0.50），在其它品种中表现为低度多态（PIC<0.25）；g.57842893C>T位点仅在苏尼特羊、萨福克羊以及杜泊羊3个品种中均处于哈代温伯格平衡状态（P>0.05）；g.57842893C>T位点与小尾寒羊第1、2以及第3胎产羔数均无显著关联（P>0.05），但CC型各胎产羔数均高于TT型。综上表明，FGF 7基因的表达水平与绵羊产羔数可能存在一定程度的正相关，虽然可能不是影响绵羊产羔数的关键基因，但g.57842893C>T位点对绵羊产羔数性状的选育具有一定的指导意义。

关键词: 绵羊, FGF7基因, 组织表达, SNP分型, 产羔数

Abstract:

To elucidate the association between tissue expression level and polymorphism of FGF 7 and litter size in sheep, semi-quantitative reverse transcription polymerase chain reaction (sqRT-PCR) and quantitative real-time PCR (qPCR) were performed to investigate the expression of FGF 7 gene in multiparous Small Tail Han sheep and uniparous Sunite sheep and different genotypes of FecB gene in Small Tail Han sheep. Multiparous (380 Small Tail Han sheep) and uniparous (total 380 for Tan, Sunite, Suffolk, Dorper and Prairie Tibetan sheep) sheep breeds were selected, and the locus of g.57842893C>T in FGF 7 gene was genotyped using Sequenom MassARRAY^® SNP assay. Then the association was analyzed between FGF 7 gene and litter size in Small Tail Han sheep. The results showed that the expression of FGF 7 was at high level in heart and lung in both uniparous and multiparous sheep breeds and at moderate or low level in other tissues. The expression of FGF 7 was higher in most tissues in multiparous Small Tail Han sheep than in uniparous Sunite sheep, but the difference was not significant (P>0.05), while the expression of FGF 7 had no obvious differences among 3 genotypes of FecB gene in Small Tail Han sheep. Three genotypes (CC, CT and TT) were found at g.57842893C>T locus in both uniparous and multiparous sheep, both genotype frequency and allele frequency reached significant difference between uniparous and multiparous sheep (P ≤ 0.05). Population genetic analysis indicated that the g.57842893C>T locus was at moderate polymorphism (0.25 < PIC < 0.50) in Sunite, Suffolk and Dorper and at low polymorphism (PIC<0.25) in other sheep breeds; χ² test revealed that the g.57842893C>T locus was under Hardy-Weinberg equilibrium in Sunite, Suffolk and Dorper sheep (P>0.05). Association analysis indicated that the g.57842893C>T locus didn't have significant correlation with the litter size of the first, second or third parity in Small Tail Han sheep (P>0.05), while the litter size of individuals with CC was higher than those with TT for each parity. Therefore, we concluded that there might be a certain positive correlation between the expression level of FGF 7and the litter size in sheep, although it may not be the key gene for litter size, the g.57842893C>T locus might provide a basis for litter size trait selection in sheep.

Key words: sheep, FGF7 gene, tissue expression, SNP genotyping, litter size

中图分类号:

S826.2

周梅, 曹晓涵, 贺小云, 孙庆, 狄冉, 胡文萍, 王翔宇, 张效生, 张金龙, 刘秋月, 储明星. 绵羊FGF7基因组织表达及其多态性与产羔数之间的关系[J]. 畜牧兽医学报, 2018, 49(3): 525-533.

ZHOU Mei, CAO Xiao-han, HE Xiao-yun, SUN Qing, DI Ran, HU Wen-ping, WANG Xiang-yu, ZHANG Xiao-sheng, ZHANG Jin-long, LIU Qiu-yue, CHU Ming-xing. Tissue Expression and Polymorphism of Sheep FGF 7 Gene and Their Association with Litter Size[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 525-533.

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绵羊FGF7基因组织表达及其多态性与产羔数之间的关系

Tissue Expression and Polymorphism of Sheep FGF 7 Gene and Their Association with Litter Size

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