日粮脂肪水平对湖羊双胞胎公羔生长性能及血清指标的影响

doi:10.11843/j.issn.0366-6964.2018.02.012

摘要/Abstract

摘要：

旨在研究日粮不同脂肪水平对早期断奶双胞胎湖羊公羔生长发育、体尺指标、断奶前腹泻率及血清指标的影响。选择7日龄体重相近、健康的湖羊双胞胎公羔30对，采用配对试验设计，分为正常脂肪组（NF）和高脂肪组（HF），一对双胞胎随机分到两个组中。7日龄断母乳，NF组开始饲喂脂肪含量为15%的代乳品和2.8%的开食料，HF组开始饲喂脂肪含量27%的代乳品及5.0%的开食料，饲喂至60日龄断液体饲料（代乳品）。60～120日龄统一饲喂开食料（US）。所有羔羊定期晨饲前称重，记录每日采食量及腹泻情况；在60、90及120日龄晨饲前颈静脉采血，用于测定血清学常规指标。结果表明：1）在30、50、90及120日龄时，HF组羔羊体重显著高于NF组（P<0.05），而断代乳品时，HF组羔羊体重有显著高于NF组的趋势（0.05 < P < 0.10）。在7～60日龄阶段，HF组代乳品平均采食量显著高于NF组（P<0.05），但开食料采食量、平均日增重及饲料转化率在两组间差异不显著（P>0.05）；在60～90日龄阶段，HF组平均日增重及开食料采食量显著高于NF组（P<0.05），而饲料转化率HF组显著低于NF组（P<0.05）；在90～120日龄期间，HF组羔羊采食量显著高于NF组（P<0.05），而饲料转化率有显著高于NF组的趋势（0.05 < P < 0.10）。在整个试验期，HF组总平均采食量显著高于NF组（P<0.05），平均日增重及饲料转化率在两组间差异不显著（P>0.05）。2）试验期内HF组羔羊断奶前腹泻率有显著低于NF组的趋势（0.05 < P < 0.10）。试验期间两组羔羊体尺指标基本上无显著差异（P>0.05）。断奶前饲喂高脂肪日粮可以在一定程度上提高湖羊双胞胎公羔断奶前后的生长性能；高脂肪日粮可以降低断代乳品前腹泻率；且本试验条件下高脂肪日粮对羔羊血液指标的影响在其自身调节的范围内。

关键词: 湖羊, 双胞胎, 脂肪水平, 生长性能, 血清指标

Abstract:

This study aimed to investigate the effects of dietary fat levels on growth, body size, preweaning diarrhea and serum parameters of early-weaned male twin Hu lambs. Thirty pairs of heathy male twin Hu lambs weaned at 7 days of age were selected and randomly allotted to two groups according to the paired design. One group was fed normal fat level (NF) diet with milk replacer (EE:15%) and starter (EE:2.8%), and another group was fed high fat level (HF) diet with milk replacer (EE:27%) and starter (EE:5%). Milk replacer and starter were fed to lambs from weaning to 60 days of age. Then lambs in NF and HF groups were fed unified starter (US) from 60 to 120 days of age. Body weight of all lambs was measured before morning feeding, daily feed intake and diarrhea status were recorded; At 60, 90 and 120 days old, blood samples were collected from jugular vein before morning feeding, and the blood routine indices were determined.The results showed that:1) Body weight of lambs in HF group was significantly higher than that in NF group on 30,50, 90 and 120 days of age (P<0.05). The weaning BW of the lambs in the HF group tended to be higher than that in the NF group (0.05 < P < 0.10). From 7 to 60 days of age, average intake of milk replacer in HF group was significantly higher than that in NF group (P<0.05), but intake of starter, average daily gain and feed conversion ratio were not significant (P>0.05) between the two groups. From 60 to 90 days of age, the ADG and intake of starter of lambs in HF group were significantly higher than that in NF group (P<0.05). Feed conversion ratio of lambs in HF group was significantly lower than that in NF group(P<0.05). From 90 to 120 days of age, intake of starter of lambs in HF group was significantly higher than that in NF group (P<0.05), the feed conversion ratio of the lambs in the HF group tended to be higher than that in the NF group (0.05 < P < 0.10). During the whole experiment period, milk replacer and starter intake of lambs in HF group were significantly higher than that in NF group (P<0.05), but the ADG and feed conversation ratio were not significantly different (P>0.05) between the two groups. 2) The diarrhea rate before weaning in HF group tended to be lower than that in NF group (0.05 < P < 0.10). There was no significant difference in body size indices between the two groups(P>0.05). High fat diet can improve the growth performance of male twin Hu lambs before and after weaning. The high fat diet can reduce the diarrhea rate before weaning, and in the present experimental condition, high fat diet have less effect on serum parameters of male twin Hu lambs, which are all within permissible values by their autoregulation.

Key words: Hu lambs, twins, fat level, growth performance, serum index

中图分类号:

S826.5

李文娟, 陶慧, 张蓉, 张帆, 解彪, 张乃锋, 刁其玉. 日粮脂肪水平对湖羊双胞胎公羔生长性能及血清指标的影响[J]. 畜牧兽医学报, 2018, 49(2): 327-337.

LI Wen-juan, TAO Hui, ZHANG Rong, ZHANG Fan, XIE Biao, ZHANG Nai-feng, DIAO Qi-yu. Effects of Dietary Fat Levels on Growth Performance and Serum Parameters of Early-weaned Male Twin Hu Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 327-337.

参考文献

[1] 祁敏丽, 马铁伟, 刁其玉, 等. 饲粮营养限制对断奶湖羊羔羊生长、屠宰性能以及器官发育的影响[J]. 畜牧兽医学报, 2016, 47(8):1601-1609.
QI M L, MA T W, DIAO Q Y, et al. Effect of protein and energy restriction on growth and slaughter performance and visceral organs development of weaned Hu lambs[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(8):1601-1609. (in Chinese)
[2] 祁敏丽, 刁其玉, 马铁伟, 等. 饲粮营养限制对羔羊肠道组织形态以及血清胰岛素样生长因子-1和胰高血糖素样-2浓度的影响[J]. 动物营养学报, 2017, 29(2):426-435.
QI M L, DIAO Q Y, MA T W, et al. Effects of dietary nutrition restrictions on intestinal morphology and serum insulin-like growth factor-1 and glucagon-like peptide-2 concentrations of lambs[J]. Chinese Journal of Animal Nutrition, 2017, 29(2):426-435. (in Chinese)
[3] WANG F B, LI C, LI F D, et al. Effects of starter feeding and early weaning on GHR mRNA expression in liver and rumen of lambs from birth to 84 days of age[J]. Arch Anim Nutr, 2016, 70(3):239-248.
[4] 柴建民, 刁其玉, 屠焰, 等. 早期断奶时间对湖羊羔羊组织器官发育、屠宰性能和肉品质的影响[J]. 动物营养学报, 2014, 26(7):1838-1847.
CHAI J M, DIAO Q Y, TU Y, et al. Effects of early weaning time on tissue and organ development, slaughter performance and meat quality of Hu lambs[J]. Chinese Journal of Animal Nutrition, 2014, 26(7):1838-1847. (in Chinese)
[5] 王波, 柴建民, 王海超, 等. 蛋白水平对早期断奶双胞胎湖羊公羔营养物质消化与血清指标的影响[J]. 畜牧兽医学报, 2016, 47(6):1170-1179.
WANG B, CHAI J M, WANG H C, et al. Effects of protein levels on nutrient digestion and metabolism and serum parameters of early-weaned male Hu twin lambs[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(6):1170-1179. (in Chinese)
[6] 祁敏丽, 柴建民, 王波, 等. 饲粮营养限制对早期断奶湖羊羔羊生长性能以及内脏器官发育的影响[J]. 动物营养学报, 2016, 28(2):444-454.
QI M L, CHAI J M, WANG B, et al. effects of nutritional restriction on growth performance and visceral organ development of early-weaned Hu lambs[J]. Chinese Journal of Animal Nutrition, 2016, 28(2):444-454. (in Chinese)
[7] 江喜春, 夏伦志, 张乃锋, 等. 代乳粉能量水平对早期断奶湖羊羔羊生长性能和物质代谢的影响[J]. 中国畜牧杂志, 2015, 51(7):50-53.
JIANG X C, XIA L Z, ZHANG N F, et al. Effect of energy level of milk replacer on growth performance and nutrient utilization in Hu lambs[J]. Chinese Journal of Animal Science, 2015, 51(7):50-53. (in Chinese)
[8] 刘涛, 富俊才, 李泽, 等. 不同脂肪水平代乳料对哺乳羔羊生长性能的影响[J]. 中国畜牧杂志, 2012, 48(13):40-43.
LIU T, FU J C, LI Z, et al. Effect of milk replacer with different fat levels on growth performance of suckling lambs[J]. Chinese Journal of Animal Science, 2012, 48(13):40-43. (in Chinese)
[9] 屠焰, 刁其玉, 岳喜新. 一种0-3月龄羔羊的代乳品及其制备方法:中国, CN201210365927.6[P]. 2013-01-30.
TU Y, DIAO Q Y, YUE X X. Milk replacer of 0-3 month lambs and production method thereof:CN, CN201210365927.6[P]. 2013-01-30. (in Chinese)
[10] NRC. Nutrient requirements of small ruminants:sheep, goats, cervids, and new world camelids[M]. Washington, D.C.:National Academies Press, 2007.
[11] 贾宇. 代乳粉对天府肉羊羔羊早期增重的影响及母羊乳成分变化规律的分析[D]. 雅安:四川农业大学, 2013.
JIA Y. Impact on early weight gain of Tianfu kids and variation of milk composition of ewes[D]. Ya'an:Sichuan Agricultural University, 2013. (in Chinese)
[12] 中华人民共和国卫生部. GB/T 5413.3-2010食品安全国家标准婴幼儿食品和乳品中脂肪的测定[S]. 北京:中国标准出版社, 2010.
Ministry of Health of the People's Republic of China. GB/T 5413.3-2010 Determination of fat in foods for infants and young children, milk and milk products[S]. Beijing:Standards Press of China, 2010. (in Chinese)
[13] 张丽英. 饲料分析及饲料质量检测技术[M]. 3版. 北京:中国农业大学出版社, 2007.
ZHANG L Y. Feed analysis and quality in inspection technology[M]. 3rd ed. Beijing:China Agricultural University Press, 2007. (in Chinese)
[14] 王若丞, 纪宇, 孙玲伟, 等. 灌服N-氨甲酰谷氨酸对哺乳山羊羔羊生长性能、血液参数及器官重的影响[J]. 动物营养学报, 2016, 28(6):1765-1773.
WANG R C, JI Y, SUN L W, et al. Effects of oral N-carbamylglutamate supplementation on growth performance, blood parameters and organ weight of suckling kidlets[J]. Chinese Journal of Animal Nutrition, 2016, 28(6):1765-1773. (in Chinese)
[15] 王海超, 张乃锋, 柴建民, 等. 人工哺育代乳粉对湖羊双胎羔羊生长发育、营养物质消化和血清学指标的影响[J]. 动物营养学报, 2015, 27(2):436-447.
WANG H C, ZHANG N F, CHAI J M, et al. effects of artificial rearing of milk replacer on growth and development, nutrient digestion and serum indices of Hu twin lambs[J]. Chinese Journal of Animal Nutrition, 2015, 27(2):436-447. (in Chinese)
[16] 王波, 柴建民, 王海超, 等. 蛋白水平对早期断奶双胞胎湖羊公羔营养物质消化与血清指标的影响[J]. 畜牧兽医学报, 2016, 47(6):1170-1179.
WANG B, CHAI J M, WANG H C, et al. Effects of protein levels on nutrient digestion and metabolism and serum parameters of early-weaned male Hu twin lambs[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(6):1170-1179. (in Chinese)
[17] BYRNE C J, FAIR S, ENGLISH A M, et al. Effect of milk replacer and concentrate intake on growth rate, feeding behaviour and systemic metabolite concentrations of pre-weaned bull calves of two dairy breeds[J]. Animal, 2017, 11(9):1531-1538.
[18] 蒋银屏, 黄剑飞, 王庆, 等. 脂肪营养及其在猪生产中的应用[J]. 广东饲料, 2014, 23(4):36-38.
JIANG Y P, HUANG J F, WANG Q, et al. Fat nutrition and its application in pig production[J]. Guangdong Feed, 2014, 23(4):36-38. (in Chinese)
[19] 蒋湘辉, 骆小年, 金广海, 等. 不同蛋白质和脂肪水平饲料对拉氏幼鱼生长、体成分和免疫力的影响[J]. 动物营养学报, 2016, 28(3):864-871.
JIANG X H, LUO X N, JIN G H, et al. Effects of different protein and lipid level diets on growth, body composition and immunity of juvenile Phoxinus lagowskii[J]. Chinese Journal of Animal Nutrition, 2016, 28(3):864-871. (in Chinese)
[20] 张铁涛, 王卓, 郭强, 等. 饲粮脂肪水平对繁殖期蓝狐繁殖性能、营养物质消化率、氮代谢及产后体重的影响[J]. 动物营养学报, 2014, 26(7):1848-1855.
ZHANG T T, WANG Z, GUO Q, et al. Effects of dietary fat level on reproduction performance, nutrient digestibility, nitrogen metabolism and postpartum weight of blue fox at the period of reproduction[J]. Chinese Journal of Animal Nutrition, 2014, 26(7):1848-1855. (in Chinese)
[21] HAVLIN J M, ROBINSON P H, KARGES K. Impacts of dietary fat level and saturation when feeding distillers grains to high producing dairy cows[J]. J Anim Physiol Anim Nutr, 2015, 99(3):577-590.
[22] KUEHN C S, OTTERBY D E, LINN J G, et al. The effect of dietary energy concentration on calf performance[J]. J Dairy Sci, 1994, 77(9):2621-2629.
[23] SILBERBAUER C J, JACOBER B, LANGHANS W. Dietary fat level and short-term effects of a high-fat meal on food intake and metabolism[J]. Ann Nutr Metab, 1998, 42(2):75-89.
[24] ARAUJO G, TERRÉ M, BACH A. Interaction between milk allowance and fat content of the starter feed on performance of Holstein calves[J]. J Dairy Sci, 2014, 97(10):6511-6518.
[25] 陈代文, 吴秀群, 张克英, 等. 不同种类脂肪对早期断奶仔猪生产性能的影响[J]. 四川畜牧兽医, 2003, 30(S1):35-37.
CHEN D W, WU X Q, ZHANG K Y, et al. Effect of different fats on growth performance of early-weaned pigs[J]. Sichuan Animal & Veterinary Sciences, 2003, 30(S1):35-37. (in Chinese)
[26] 刘忠臣, 陈代文, 余冰, 等. 不同脂肪来源对断奶仔猪生长性能和脂类代谢的影响[J]. 动物营养学报, 2011, 23(9):1466-1474.
LIU Z C, CHEN D W, YU B, et al. Effect of different fat sources on growth performance and lipid metabolism in weaned piglets[J]. Chinese Journal of Animal Nutrition, 2011, 23(9):1466-1474. (in Chinese)
[27] 郑荷花, 李伟, 张莉莉, 等. 脂肪粉对断奶仔猪生产性能、养分利用率和血清生化指标的影响[J]. 畜牧与兽医, 2013, 45(6):14-18.
ZHENG H H, LI W, ZHANG L L, et al. Effects of fat powders on growth performance, nutrients digestibility and serum biochemical parameters in weaned piglets[J]. Animal Husbandry & Veterinary Medicine, 2013, 45(6):14-18. (in Chinese)
[28] TIKOFSKY J N, VAN AMBURGH M E, ROSS D A. Effect of varying carbohydrate and fat content of milk replacer on body composition of Holstein bull calves[J]. J Anim Sci, 2001, 79(9):2260-2267.
[29] FERNANDES S R, MONTEIRO A L G, DITTRICH R L, et al. Early weaning and concentrate supplementation on the performance and metabolic profile of grazing lambs[J]. Rev Bras Zootecn, 2012, 41(5):1292-1300.
[30] WATERMAN R C, GEARY T W, PATERSON J A, et al. Early weaning in Northern Great Plains beef cattle production systems:I. Performance and reproductive response in range beef cows[J]. Livest Sci, 2012, 148(1-2):26-35.
[31] 陈琨飞. 不同脂肪来源的日粮对仔猪生长发育性能、血液生化指标及免疫功能的影响[D]. 南京:南京农业大学, 2012.
CHEN K F. Effect of different fat source in diet to piglet on its performance and blood biochemical indexes and immunity[D]. Nanjing:Nanjing Agricultural University, 2012. (in Chinese)
[32] HILL T M, VANDEHAAR M J, SORDILLO L M, et al. Fatty acid intake alters growth and immunity in milk-fed calves[J]. J Dairy Sci, 2011, 94(8):3936-3948.
[33] HILL T M, ALDRICH J M, SCHLOTTERBECK R L, et al. Amino acids, fatty acids, and fat sources for calf milk replacers[J]. Profess Anim Sci, 2007, 23(4):401-408.
[34] 邓波, 吴杰, 郭红炳, 等. 饲粮添加硬脂酰乳酸钠对断奶仔猪生长性能、血清生化指标及养分表观消化率的影响[J]. 动物营养学报, 2017, 29(2):449-455.
DENG B, WU J, GUO H B, et al. Effects of dietary sodium stearyl lactate supplementation on growth performance, serum biochemical indices and nutrient apparent digestibility of weaning piglets[J]. Chinese Journal of Animal Nutrition, 2017, 29(2):449-455. (in Chinese)
[35] 李雪玲, 张乃锋, 马涛, 等. 开食料中赖氨酸、蛋氨酸、苏氨酸和色氨酸对断奶羔羊生长性能、氮利用率和血清指标的影响[J]. 畜牧兽医学报, 2017, 48(4):678-689.
LI X L, ZHANG N F, MA T, et al. Effects of lysine, methionine, threonine and tryptophan in starter on growth performance, nitrogen utilization and serum parameters in weaned lambs[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(4):678-689. (in Chinese)