基因Ⅰ型猪乙型脑炎灭活疫苗免疫原性评价

doi:10.11843/j.issn.0366-6964.2017.05.013

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (5): 889-895.doi: 10.11843/j.issn.0366-6964.2017.05.013

基因Ⅰ型猪乙型脑炎灭活疫苗免疫原性评价

冯瑶, 岳辉喜, 刘洪明, 曹三杰, 伍锐, 黄小波, 文翼平, 赵勤, 文心田^*

四川农业大学动物医学院猪病研究中心, 成都 611130

收稿日期:2016-12-01 出版日期:2017-05-23 发布日期:2017-05-20
通讯作者: 文心田,教授,博士生导师,E-mail:xintian3211@126.com
作者简介:冯瑶(1991-),女,四川广安人,硕士生,主要从事猪基因Ⅰ型乙型脑炎病毒疫苗的研究,E-mail:fengyao1005@163.com
基金资助:
公益性行业（农业）科研专项（201203082）

The Immunogenicity Evaluation of Inactivated Vaccines of Swine Japanese Encephalitis Virus Genetype Ⅰ

FENG Yao, YUE Hui-xi, LIU Hong-ming, CAO San-jie, WU Rui, HUANG Xiao-bo, WEN Yi-ping, ZHAO Qin, WEN Xin-tian^*

Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-12-01 Online:2017-05-23 Published:2017-05-20

摘要/Abstract

摘要：

为了评估猪基因Ⅰ型乙型脑炎病毒YA1株、CZ株灭活疫苗的免疫原性，通过将YA1株、CZ株乙脑病毒分别在乳鼠脑内连续传代，获得了高病毒含量的鼠脑病毒液。将佐剂ISA206分别与灭活的YA1株、CZ株乙脑病毒液混合，制备成鼠脑灭活苗。将YA1株、CZ株鼠脑灭活苗以及HW1株商品化鼠脑灭活苗分别免疫接种小鼠、豚鼠、仔猪，采用间接ELISA试验、血凝抑制试验、病毒中和试验以及攻毒保护试验分别检测IgG抗体、血凝抑制抗体、中和抗体的的产生情况以及疫苗对小鼠的攻毒保护率。结果显示YA1株、CZ株灭活苗在小鼠和仔猪上产生的IgG抗体、在豚鼠上产生的血凝抑制抗体、在仔猪上产生的中和抗体以及对小鼠的攻毒保护率均高于HW1株。结果表明乙脑病毒YA1株、CZ株灭活苗的免疫原性优于HW1株，其中CZ株灭活苗的免疫原性最优。

关键词: 乙型脑炎病毒, 基因Ⅰ型, 基因Ⅲ型, 灭活苗, 免疫原性

Abstract:

The objective of this study was to evaluate the immunogenicity of inactivated vaccines of swine Japanese encephalitis virus genetype Ⅰ of YA1 and CZ strains. High titer viruses were obtained by serial passages on suckling mouse brains. Then inactivated vaccines of YA1 and CZ strains were prepared by mixing these inactivated viruses with adjuvant ISA206, respectively. Furthermore, the immunogenicity of inactivated vaccines were evaluated in mice, guinea pigs and piglets. IgG antibody, hemagglutination inhibition antibody, plaque reduction neutralizing antibody and vaccine protection rate were detected through indirect ELISA test, hemagglutination inhibition test, plaque reduction neutralization test and protection rate test, respectively. These results showed that inactivated vaccines of YA1 and CZ strains produced higher IgG antibody, hemagglutination inhibition antibody, plaque reduction neutralizing antibody, and vaccine protective rate than HW1 strain in these experimental animals. These results indicated that the immunogenicity of inactivated vaccines of YA1, CZ strains was better than inactivated vaccine of HW1 strain. Above all, the immunogenicity of the inactivated vaccine of CZ strain was the best.

Key words: Japanese encephalitis virus, genetype Ⅰ, genetype Ⅲ, inactivated vaccine, immunogenicity

中图分类号:

S813.1

冯瑶, 岳辉喜, 刘洪明, 曹三杰, 伍锐, 黄小波, 文翼平, 赵勤, 文心田. 基因Ⅰ型猪乙型脑炎灭活疫苗免疫原性评价[J]. 畜牧兽医学报, 2017, 48(5): 889-895.

FENG Yao, YUE Hui-xi, LIU Hong-ming, CAO San-jie, WU Rui, HUANG Xiao-bo, WEN Yi-ping, ZHAO Qin, WEN Xin-tian. The Immunogenicity Evaluation of Inactivated Vaccines of Swine Japanese Encephalitis Virus Genetype Ⅰ[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 889-895.

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基因Ⅰ型猪乙型脑炎灭活疫苗免疫原性评价

The Immunogenicity Evaluation of Inactivated Vaccines of Swine Japanese Encephalitis Virus Genetype Ⅰ

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