镰刀菌毒素对断奶仔猪肠道IL-1β和IL-6分布和表达的影响

doi:10.11843/j.issn.0366-6964.2016.10.022

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (10): 2126-2135.doi: 10.11843/j.issn.0366-6964.2016.10.022

镰刀菌毒素对断奶仔猪肠道IL-1β和IL-6分布和表达的影响

陈祥兴¹，张崇玉²，黄丽波²，刘法孝²，杨维仁^2*，姜淑贞^2*

（1.南京农业大学动物科技学院，南京 210095；2.山东农业大学动物科技学院，泰安 271000）

收稿日期:2016-05-11 出版日期:2016-10-23 发布日期:2016-10-24
通讯作者: 姜淑贞，副教授，硕士生导师，E-mail: shuzhen305@163.com；杨维仁，教授，博士生导师，E-mail: wryang@sdau.edu.cn
作者简介:陈祥兴(1988-)，男，山东淄博人，博士生，主要从事动物营养与饲料科学研究，Tel：0538-8241257，E-mail: cxxabcd@163.com
基金资助:
山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-05)

Effects of Fusarium Toxins on IL-1β and IL-6 in Post-weaning Piglets

CHEN Xiang-xing¹，ZHANG Chong-yu²，HUANG Li-bo²，LIU Fa-xiao²，YANG Wei-ren^2* ，JIANG Shu-zhen^2*

(1.College of Animal Science and Technology，Nanjing Agricultural University，Nanjing 210095，China；2.College of Animal Science and Veterinary Medicine，Shandong Agricultural University，Tai’an 271000，China)

Received:2016-05-11 Online:2016-10-23 Published:2016-10-24

摘要/Abstract

摘要：

旨在探讨镰刀菌毒素对断奶仔猪肠道IL-1β和IL-6的分布和mRNA转录量的影响。选择35日龄体重为8.45 kg±0.94 kg的健康三元杂交（杜×长×大）雌性仔猪40头，随机分为2个处理组，每组20头。对照组饲喂基础饲粮，镰刀菌毒素组饲喂含有镰刀菌毒素（玉米赤霉烯酮0.90 mg•kg^-1，呕吐毒素1.43 mg•kg^-1，烟曲霉毒素5.85 mg•kg^-1）的试验饲粮，预试期7 d，正试期35 d，试验结束每组随机选取10头仔猪进行屠宰取样。结果表明：与对照组相比，镰刀菌毒素组仔猪小肠IL-1β和IL-6的阳性细胞呈现由分散在固有层向绒毛内淋巴细胞集中的趋势，而结肠的IL-1β和IL-6阳性细胞主要分布在固有层；十二指肠IL-1β、十二指肠和空肠的IL-6以及结肠IL-1β和IL-6的mRNA转录量显著高于对照组（P<0.05），且小肠和结肠IL-1β和IL-6 mRNA转录量存在一定相关性（P<0.05）。在本试验条件下，镰刀菌毒素可通过改变肠道IL-1β和IL-6的分布和表达，影响肠道的免疫机能。

关键词: 镰刀菌毒素, 肠道, IL-1&beta, IL-6, 仔猪

Abstract:

The aim of the present study was to investigate effects of Fusarium toxins on distribution and mRNA expression levels of intestinal IL-1β and IL-6 in post-weaning piglets.A total of 40 healthy piglets (Duroc×Landrace×Large White) aged at 35 d with an average body weight (8.45±0.94) kg were randomly allocated into two treatments with 20 in the control group (Control) and 20 in the test group (Fusarium Toxins).Piglets in the control group were fed a basal diet only，and piglets in the Fusarium toxins group were fed a test diet (0.90 mg•kg^-1 ZEN，1.43 mg•kg^-1 DON，5.85 mg•kg^-1 FUM).The experimental period was 35 d after 7 d adaptation.Then 10 piglets of each treatment were chosen to slaughter and sample.Results showed because of the Fusarium toxins，the positive cells of the small intestinal IL-1β and IL-6 were from dispersed in the lamina propria to villous lymphocytes concentration.But the IL-1β and IL-6 positive cells of the colon were mainly distributed in the lamina propria cells.And the IL-1β mRNA levels of duodenum，the IL-6 mRNA levels of duodenum and jejunum and the IL-1β and IL-6 mRNA levels of colon of the Fusarium toxins group were significantly higher (P<0.05) than that of the control.The mRNA levels of IL-1β and IL-6 had a correlation between small intestine and colon.In the present study，it suggested that Fusarium toxins exerted a deleterious effect on inflammatory response via distribution and mRNA expression levels of intestinal IL-1β and IL-6，which have a negative impact on immunity of piglets eventually.

Key words: Fusarium toxins, intestine, IL-1β, IL-6, piglets

中图分类号:

S859.87

陈祥兴，张崇玉，黄丽波，刘法孝，杨维仁，姜淑贞. 镰刀菌毒素对断奶仔猪肠道IL-1β和IL-6分布和表达的影响[J]. 畜牧兽医学报, 2016, 47(10): 2126-2135.

CHEN Xiang-xing，ZHANG Chong-yu，HUANG Li-bo，LIU Fa-xiao，YANG Wei-ren，JIANG Shu-zhen. Effects of Fusarium Toxins on IL-1β and IL-6 in Post-weaning Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2126-2135.

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镰刀菌毒素对断奶仔猪肠道IL-1β和IL-6分布和表达的影响

Effects of Fusarium Toxins on IL-1β and IL-6 in Post-weaning Piglets

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