多杀性巴氏杆菌脂多糖的结构与功能研究进展

doi:doi: 10.11843/j.issn.0366-6964.2016.10.003

摘要/Abstract

摘要：

多杀性巴氏杆菌是一种兼性厌氧的革兰阴性菌，能够造成多种动物的巴氏杆菌病。脂多糖（lipopolysaccharide，LPS）既是细菌重要的毒力因子，又是主要的保护性抗原。依据LPS血清学反应，多杀性巴氏杆菌可分为16个血清型。与大多数革兰阴性菌不同，多杀性巴氏杆菌的脂多糖不含O-抗原，仅由类脂A和核心寡糖两部分组成。近年来人们通过质谱检测和基因测序陆续揭示了这16个血清型核心寡糖的化学结构和合成基因。研究表明，各血清型内核心寡糖结构十分保守，合成基因分散存在于基因组；外核心寡糖的化学组成具有多样性，其合成基因成簇存在，形成外核心寡糖基因簇。虽然有些血清型共享同样的外核心寡糖基因簇，但由于基因突变造成它们外核心寡糖结构的异质性。研究还发现核心寡糖的结构与多杀性巴氏杆菌的毒力有关。作者在本文中综述了多杀性巴氏杆菌16个血清型的核心寡糖的化学结构、基因组成及其结构与毒力的关系，为多杀性巴氏杆菌病的防治提供借鉴。

关键词: 多杀性巴氏杆菌, 脂多糖, 化学结构, 基因组成, 毒力

Abstract:

Pasteurella multocida (P.multocida)，a Gram-negative cocco-bacillus，facultative anaerobic bacterium，is the causative agent of serious diseases in a wide range of animals.Lipopolysaccharide (LPS) is an important virulence factor，and is also a major immune protective antigen of P.multocida which is currently classified into 16 Heddleston serovars based on LPS.Unlike the other Gram-negative pathogens，P.multocida LPS is only made up of lipid A and core oligosaccharide，lacking an O-antigen.Recently，chemical structures and biosynthesis genes of 16 Heddleston serovars LPS have been determined by using mass spectrometry and gene sequencing.The inner core structures are highly conserved among different serovars，and genes required for the assembly of the inner core are located in several regions of the genome.In contrast，the outer core structures are distinct，and genes required for the biosythesis of the outer core structures are clustered in a single locus between the conserved genes priA and fpg.Some LPS serovars genetically have relationship each other，sharing the same outer core biosythesis locus，but producing different LPS molecules due to mutations within glycosyltransferase genes.Furthermore，LPS structures of P.multocida are related to bacterial virulence.Here，we summarize LPS structures，biosythesis genes and the relationships between LPS and bacterial virulence in P.multocida.

Key words: Pasteurella multocida, lipopolysaccharide, chemical structures, biosynthesis genes, virulence

中图分类号:

R378.6

华瑞其，赵新新，程安春. 多杀性巴氏杆菌脂多糖的结构与功能研究进展[J]. 畜牧兽医学报, 2016, 47(10): 1961-1968.

HUA Rui-qi，ZHAO Xin-xin，CHENG An-chun. Research Progress in the Lipopolysaccharide of Pasteurella multocida[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1961-1968.

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