鸭源鸡杆菌整合子及其与耐药性的相关性分析

doi:doi: 10.11843/j.issn.0366-6964.2016.08.019

摘要/Abstract

摘要：

为了解鸭源鸡杆菌的整合子及其与耐药性之间的关系，运用PCR方法检测鸭源鸡杆菌分离株携带1、2、3类整合子的情况，采用K-B法测定61株鸭源鸡杆菌对13种抗菌药的敏感性，并分析其整合子与耐药性的关联性。结果显示，所有参试菌株对克林霉素、诺氟沙星、链霉素有耐药性的菌株比例均不低于88.52 %（54/61）。19株鸭源鸡杆菌携带1类整合子，阳性率为31.15%，且此类菌株的耐药种数均≥3；在耐药种数≥5的菌株中，整合子阳性菌的比例（78.95%）显著高于整合子阴性菌的比例（28.57%，P＜0.01）。本试验首次研究了鸭源鸡杆菌中整合子的分布情况。参试菌株仅携带1类整合子，且普遍存在多重耐药性；1类整合子可能在鸭源鸡杆菌多重耐药性的形成中发挥一定作用。

关键词: 鸭源鸡杆菌, 整合子, 耐药性

Abstract:

The aim of the present study was to confirm existence of the integrons in Gallibacterium anatis（G.anatis）isolates and the role of integrons in multidrug resistance.The integrons class 1，2 and 3 in G.anatis isolates were detected by PCR amplification for the first time.The sensitivity of 61 G.anatis strains was tested against 13 kinds of antibiotics by Kirby-Bauer method.The correlation between multidrug resistance and integrons was analyzed.The results showed that drug resistance rates of all G.anatis isolates to clindamycin，norfloxacin and streptomycin were above 88.52%.No less than three kinds of drugs were resisted for 19 G.anatis strains which carrying class 1 integron.Among strains resisting more than five kinds of drugs，the proportion of the class 1 integron positive (78.95%) was significantly higher than that（28.57%）of class 1 integron negative strains（P＜0.01）.It was the first time，to our knowledge，that the distribution of intergons in G.anatis had been investigated. Only class 1 integron was found and multidrug resistance was very common in G.anatis isolates in this study.Class 1 integron might play an important role in the formation of multiple drug resistance phenotype of G.anatis strains.

Key words: Gallibacterium anatis, antibiotic resistance, integrons

中图分类号:

S852.612

彭志锋，高冬生，刘红英，杨霞，赵军，王新卫，李永涛，陈陆，常洪涛，王川庆. 鸭源鸡杆菌整合子及其与耐药性的相关性分析[J]. 畜牧兽医学报, 2016, 47(8): 1676-1681.

PENG Zhi-feng，GAO Dong-sheng，LIU Hong-ying，YANG Xia，ZHAO Jun，WANG Xin-wei，LI Yong-tao，CHEN Lu，CHANG Hong-tao，WANG Chuan-qing. Gallibacterium anatis Integron and Its Correlation with the Drug Resistance[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1676-1681.

参考文献

［1］CHRISTENSEN H，BISGAARD M，BOJESEN A M，et al.Genetic relationships among avian isolates classified as Pasteurella haemolytica，‘Actinobacillus salpingitidis’ or Pasteurella anatis with proposal of Gallibacterium anatis gen.nov.，comb.nov.and description of additional genomospecies within Gallibacterium gen.nov［J］.Int J Syst Evol Microbiol，2003，53(Pt 1)：275-287.
［2］BISGAARD M，KORCZAK B M，BUSSE H J，et al.Classification of the taxon 2 and taxon 3 complex of Bisgaard within Gallibacterium and description of Gallibacterium melopsittaci sp.nov.，Gallibacterium trehalosifermentans sp.nov.and Gallibacterium salpingitidis sp.nov［J］.Int J Syst Evol Microbiol，2009，59(Pt 4)：735-744.
［3］PAUDEL S，LIEBHART D，HESS M，et al.Pathogenesis of Gallibacterium anatis in a natural infection model fulfils Koch’s postulates：1.Folliculitis and drop in egg production are the predominant effects in specific pathogen free layers［J］.Avian Pathol，2014，43(5)：443-449.
［4］王川庆，陈陆，杨霞，等.蛋鸡群卡氏杆菌感染情况的初步研究［J］.河南农业科学，2008(3)：97-100，103.
WANG C Q，CHEN L，YANG X，et al.A premilinary study of Gallibacterium anatis infection in laying hens［J］.Journal of Henan Agriculatural Sciences，2008(3)：97-100，103.(in Chinese)
［5］GLASS-KAASTRA S K，PEARL D L，REID-SMITH R J，et al.Antimicrobial susceptibility of Escherichia coli F4，Pasteurella multocida，and Streptococcus suis isolates from a diagnostic veterinary laboratory and recommendations for a surveillance system［J］.Can Vet J，2014，55(4)：341-348.
［6］YAKUBU D，MOSHOOD R，PAUL A，et al.Phenotypic characteristics of Pasteurella multocida isolated from commercial chickens affected by fowl cholera in Jos，Nigeria［J］.J World’s Poult Res，2015，5(3)：58-62.
［7］BOJESEN A M，VAZQUEZ M E，BAGER R J，et al.Antimicrobial susceptibility and tetracycline resistance determinant genotyping of Gallibacterium anatis［J］.Vet Microbiol，2011，148(1)：105-110.
［8］郭伦涛，王川庆，杨霞，等.卡氏杆菌对磺胺甲噁唑及链霉素的耐药性与耐药基因关系的初步研究［J］.中国家禽，2009，31(18)：22-24.
GUO L T，WANG C Q，YANG X，et al.Study of relation between drug resistance against sulfamethoxazole and streptomycin in Gallibacterium and resistant genes［J］.China Poultry， 2009，31(18)：22-24.(in Chinese)
［9］XU Y，LUO Q Q，ZHOU M G.Identification and characterization of integron-mediated antibiotic resistance in the phytopathogen Xanthomonas oryzae pv.oryzae［J］.PLoS One，2013，8(2)：e55962.
［10］MARTINS N，PICO R C，ADAMS-SAPPER S，et al.Association of class 1 and 2 integrons with multidrug-resistant Acinetobacter baumannii international clones and Acinetobacter nosocomialis isolates［J］.Antimicrob Agents Chemother，2015，59(1)：698-701.
［11］OOSTERIK L H，PEETERS L，MUTUKU I，et al.Susceptibility of avian pathogenic Escherichia coli from laying hens in Belgium to antibiotics and disinfectants and integron prevalence［J］.Avian Dis，2014，58(2)：271-278.
［12］KEHRENBERG C，SCHWARZ S.Trimethoprim resistance in a porcine Pasteurella aerogenes isolate is based on a dfrA1 gene cassette located in a partially truncated class 2 integron［J］.J Antimicrob Chemother，2010，66(2)：450-452.
［13］郑鹿平.我国鸡群鸡杆菌的首次分离鉴定及生物学特性初步研究［D］.郑州：河南农业大学，2010.
ZHENG L P.The first isolation and identification of Gallibacterium anatis from fowls in China and preliminary research on its biological characterics［D］.Zhengzhou：Henan Agriculral University，2010.(in Chinese)
［14］PLOY M C，DENIS F，COURVALIN P，et al.Molecular characterization of integrons in Acinetobacter baumannii：description of a hybrid class 2 integron［J］.Antimicrob Agents Chemother，2000，44(10)：2684-2688.
［15］SINGER R S，WILLIAMS-NGUYEN J.Human health impacts of antibiotic use in agriculture：A push for improved causal inference［J］.Curr Opin Microbiol，2014，19：1-8.
［16］JOHNSON T J，SINGER R S，ISAACSON R E，et al.In vivo transmission of an IncA/C plasmid in Escherichia coli depends on tetracycline concentration，and acquisition of the plasmid results in a variable cost of fitness［J］.Appl Environ Microbiol，2015，81(10)：3561-3570.
［17］林志敏，林彬彬，程龙飞.禽源多杀性巴氏杆菌耐药性及其质粒多样性的研究［J］.福建农业学报，2015，30(7)：648-651.
LIN Z M，LIN B B，CHENG L F.Plasmid diversity and antibiotic resistance of avian Pasteruella multocida［J］.Fujian Journal of Agricultural Sciences，2015，30(7)：648-651.(in Chinaese)
［18］孙申菊.雪山鸡多杀性巴氏杆菌的分离鉴定与药敏试验［J］.畜禽业，2015(4)：12-13.
SUN S J.The indification of Pasteurella multocida isolated from xueshan chicken and its drug sensitive tes［J］. Livestock and Poultry Industry，2015(4)：12-13.(in Chinaese)
［19］HALL R M，STOKES H W.Integrons：novel DNA elements which capture genes by site-specific recombination［J］.Genetica，1993，90(2-3)：115-132.
［20］GILLINGS M R.Integrons：past，present，and future［J］.Microbiol Mol Biol Rev，2014，78(2)：257-277.
［21］GUERRA B，SOTO S，CAL S，et al.Antimicrobial resistance and spread of class 1 integrons among Salmonella serotypes［J］.Antimicrob Agents Chemother，2000，44(8)：2166-2169.
［22］KANG H Y，JEONG Y S，OH J Y，et al.Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea［J］.J Antimicrob Chemother，2005，55(5)：639-644.
［23］KHAN A A，PONCE E，NAWAZ M S，et al.Identification and characterization of Class 1 integron resistance gene cassettes among Salmonella strains isolated from imported seafood［J］.Appl Environ Microbiol，2009，75(4)：1192-1196.
［24］赵红霞，李培锋，吴聪明，等.奶牛子宫内膜炎大肠杆菌耐药性及其与整合子关系的研究［J］.畜牧兽医学报，2009，40(1)：142-144.
ZHAO H X，LI P F，WU C M，et al.Research of drug-resistance of Escherichia coli isolated from bovine with endometritis and the relationship between antibiotic resistance and integron［J］.Acta Veterinaria et Zootechnica Sinica，2009，40(1)：142-144.(in Chinaese)
［25］LIU H，WANG H，HUANG M，et al.Analysis of antimicrobial resistance and class 1 integrons among strains from upper respiratory tract of healthy adults［J］.J Thorac Dis，2013，5(2)：149-155.

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