几种黏膜佐剂口服免疫对鸡空肠、回肠抗体分泌细胞的影响

doi:10.11843/j.issn.0366-6964.2016.06.022

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (6): 1253-1259.doi: 10.11843/j.issn.0366-6964.2016.06.022

几种黏膜佐剂口服免疫对鸡空肠、回肠抗体分泌细胞的影响

杨晶晶，杨倩^*

(南京农业大学农业部动物生理生化重点开放实验室，南京 210095)

收稿日期:2015-11-09 出版日期:2016-06-23 发布日期:2016-06-20
通讯作者: 杨倩(1962-)，女，教授，博导，主要从事动物黏膜免疫研究，Tel：025-84395817，E-mail：zxbyq@njau.edu.cn
作者简介:杨晶晶(1984-)，女，云南通海人，硕士生，主要从事动物黏膜免疫研究，E-mail：july0108@163.com
基金资助:
国家自然科学基金（31172302）；国家公益性农业行业科研专项（200130304）

Effect of Oral Immunization with Several Adjuvants on Antibody Secreting Cells in Chicken Jejunum and Ileum

YANG Jing-jing，YANG Qian^*

(Key Laboratory of Animal Physiology and Biochemistry of Ministry of Agriculture，Nanjing Agricultural University，Nanjing 210095，China)

Received:2015-11-09 Online:2016-06-23 Published:2016-06-20

摘要/Abstract

摘要：

拟研究左旋咪唑、聚乙烯亚胺和聚肌胞分别配合灭活H5N1禽流感病毒口服免疫雏鸡，空肠、回肠中IgA和IgG抗体分泌细胞数量和分布的变化。应用左旋咪唑、聚乙烯亚胺和聚肌胞分别配合灭活H5N1禽流感病毒经口免疫雏鸡后，通过免疫组织化学方法显示空肠、回肠IgA和IgG分泌细胞。聚乙烯亚胺配合灭活禽流感病毒口服免疫后，空肠、回肠IgA和 IgG分泌细胞的数量均显著（P＜0.05）高于单独口服灭活禽流感病毒免疫后的数量；左旋咪唑和聚肌胞分别配合灭活禽流感病毒口服免疫后，空肠、回肠IgA分泌细胞数量显著（P＜0.05）增加，IgG分泌细胞数量无明显变化。研究表明，左旋咪唑、聚肌胞和聚乙烯亚胺分别配合灭活禽流感病毒口服免疫雏鸡，均能提高肠道局部体液免疫水平，聚乙烯亚胺的效果最好，价格更便宜。

关键词: PEI, 聚肌胞, 左旋咪唑, IgA分泌细胞, IgG分泌细胞

Abstract:

The experiment was conducted to investigate the effect of oral immunization with H5N1 inactivated avian influenza virus (IAIV) and adjuvant levamisole hydrochloride(LMS)，Poly(I：C)，Polyethyleneimine (PEI) respectively on the number and distribution of antibody secreting cells in chicken jejunum and ileum.Chicken were immunized through the oral route with H5N1 IAIV and adjuvants LMS，Poly(I：C) and PEI respectively，IgA and IgG secreting cells were detected by Immunohistochemistry to evaluate the immune effect.The number of IgA and IgG secreting cells in jejunum and ileum induced by PEI plus H5N1 IAIV were substantially (P<0.05) greater than antigen-alone treatment.The number of IgA secreting cells in jejunum and ileum induced by LMS and Poly(I：C) plus H5N1 IAIV were substantially (P<0.05) increased than antigen-alone treatment，the number of IgG secreting cells have no changes.Our study showed that chicken oral immunization with H5N1 IAIV and adjuvant LMS，Poly(I：C) and PEI respectively strengthen intestinal humoral immune level in intestine，but the cost of PEI was cheapest and most effective oral immune adjuvant.

Key words: PEI, poly(I：C), levamisole hydrochloride, IgA secreting cell, IgG secreting cell

中图分类号:

S852.4

杨晶晶，杨倩. 几种黏膜佐剂口服免疫对鸡空肠、回肠抗体分泌细胞的影响[J]. 畜牧兽医学报, 2016, 47(6): 1253-1259.

YANG Jing-jing，YANG Qian. Effect of Oral Immunization with Several Adjuvants on Antibody Secreting Cells in Chicken Jejunum and Ileum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1253-1259.

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几种黏膜佐剂口服免疫对鸡空肠、回肠抗体分泌细胞的影响

Effect of Oral Immunization with Several Adjuvants on Antibody Secreting Cells in Chicken Jejunum and Ileum

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