绵羊不同部位脂肪组织microRNA高通量测序及生物信息学分析

doi:10.11843/j.issn.0366-6964.2016.06.003

摘要/Abstract

摘要：

本研究旨在挖掘绵羊不同脂肪组织中特异表达的miRNAs。构建肾周(PEF)、皮下(SUF)和尾部(TAF)脂肪组织的3个小RNA文库，利用Solexa测序技术进行测序，用Stem-loop qRT-PCR技术和生物信息学方法对测序结果进行验证和分析。结果表明，8个随机选择的miRNAs中有7个miRNAs的表达与测序结果一致。在肾周和皮下脂肪组织的sRNA文库中分别检测到128和112个保守miRNAs，分别发现198和196个新miRNAs。结合前期在尾脂中检测到miRNAs的分析结果，肾周脂肪中特异表达的有12个保守的和66个新miRNAs；皮下脂肪中特异表达的保守和新miRNAs分别为1个和30个；尾脂中特异表达的保守和新miRNAs分别为1个和34个。上述结果将为进一步研究miRNA调控绵羊的脂肪代谢提供科学依据。

关键词: 绵羊, 脂肪组织, microRNA, Solexa测序, 生物信息学方法

Abstract:

This study aimed to find tissue-specifically expressed miRNAs in ovine adipose tissues.Three small RNA (sRNA) libraries from perirenal，subcutaneous and tail adipose tissues were built for Solexa sequencing.The sequencing results were validated by Stem-loop qRT-PCR technology and further analyzed by bioinformatics methods.The PCR results shown that seven in eight randomly selected miRNAs were consistent with the sequencing results.In perirenal and subcutaneous adipose tissues，128 and 112 conserved miRNAs were obtained，198 and 196 novel miRNAs were found，respectively.Combined with the previous analysis results of miRNAs detected in tail adipose tissue，12 conserved and 66 novel miRNAs were specifically expressed in perirenal adipose tissue；one conserved and 30 novel miRNAs were specifically expressed in subcutaneous adipose tissue；and one conserved and 34 novel miRNAs were specifically expressed in tail adipose tissue.These results provide scientific bases for further research on the regulatory role of miRNA in fat metabolism.

Key words: sheep, adipose tissues, microRNA, Solexa sequencing, bioinformatics methods

中图分类号:

S813.3
S826

张方，胡子乔，景炅婕，潘洋洋，乔利英，李宝钧，刘建华，刘文忠. 绵羊不同部位脂肪组织microRNA高通量测序及生物信息学分析[J]. 畜牧兽医学报, 2016, 47(6): 1093-1101.

ZHANG Fang，HU Zi-qiao，JING Jiong-jie，PAN Yang-yang，QIAO Li-ying，LI Bao-jun，LIU Jian-hua，LIU Wen-zhong. High-throughput Sequencing and Bioinformatics Analysis on microRNAs Expressed in Adipose Tissues of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1093-1101.

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