棕榈酸和硬脂酸对HepG2细胞血红素加氧酶-1和核转录因子Nrf2表达的影响

doi:10.11843/j.issn.0366-6964.2016.03.025

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (3): 603-608.doi: 10.11843/j.issn.0366-6964.2016.03.025

棕榈酸和硬脂酸对HepG2细胞血红素加氧酶-1和核转录因子Nrf2表达的影响

郝丽红，张秀英^*，张金铭，崔晓旭，孙晓琦，关舒函

(东北农业大学动物医学学院，哈尔滨 150030)

收稿日期:2015-07-10 出版日期:2016-03-23 发布日期:2016-03-21
通讯作者: 张秀英
作者简介:郝丽红(1989-)，女，黑龙江哈尔滨人，硕士生，主要从事基础兽医学研究，E-mail：15546036683@163.com
基金资助:
国家自然科学基金项目(31172369)

Effects of Palmitic Acid and Stearic Acid on Genes and Protein Expressions of Heme Oxygenase 1 and Nuclear Transcription Factor Nrf2 in HepG2 Cell

HAO Li-hong，ZHANG Xiu-ying^*，ZHANG Jin-ming，CUI Xiao-xu，SUN Xiao-qi，GUAN Shu-han

（College of Veterinary Medicine，Northeast Agricultural University，Harbin 150030，China）

Received:2015-07-10 Online:2016-03-23 Published:2016-03-21

摘要/Abstract

摘要：

研究棕榈酸(PA)和硬脂酸(SA)作用HepG2细胞后，对其血红素加氧酶-1 (HO-1)和核转录因子Nrf2在转录和蛋白质水平的影响。PA和SA分别以0.25、0.5、0.75、1.0 mmol•L^-1浓度刺激HepG2细胞24 h后，采用荧光定量PCR以及蛋白免疫印迹法(Western blotting)检测细胞HO-1和Nrf2的mRNA转录及蛋白质表达量的变化。结果表明：PA作用细胞后，与对照组相比，随着PA浓度的递增HepG2细胞中Nrf2和HO-1的mRNA转录及蛋白质的表达量均极显著增加(P＜0.01)；而SA组浓度为0.25、0.5、0.75 mmol•L^-1时，Nrf2和HO-1的mRNA转录及蛋白质的表达量与对照组相比极显著增加(P＜0.01)，当SA浓度为1.0 mmol•L^-1时其增加量相对减少。在1 mmol•L^-1范围内，较高浓度的PA和较低浓度的SA对HepG2细胞Nrf2和HO-1的mRNA转录及其蛋白质的诱导作用显著。

关键词: 棕榈酸, 硬脂酸, HepG2细胞, 血红素加氧酶-1, 核转录因子Nrf2

Abstract:

The purpose of the study was to explore the effects of Palmitic acid (PA) and Stearic acid (SA) on genes and protein expression of heme oxygenase 1 (HO-1) and Nuclear Transcription Factor Nrf2 in HepG2 Cells.The HepG2 cells were treated with PA or SA of 0.25，0.5，0.75，1.0 mmol•L^-1 for 24 h，the mRNA and protein expression levels of HO-1 and Nrf2 were detected by quantitative reverse transcription PCR and western blotting，separately.The experimental results were as follows，compared with the control group，the mRNA and protein expressions of HO-1 and Nrf2 were significantly increased with the increasing of PA concentration (P＜0.01)；While the mRNA and protein expressions of HO-1 and Nrf2 were significantly increased with SA of the concentrations of 0.25，0.5，0.75 mmol•L^-1 (P＜0.01)，the increased amount of the mRNA and protein expressions of HO-1 and Nrf2 were relatively decreased when the concentration of SA was 1.0 mmol•L^-1.In conclusion，a relatively higher concentration of PA and a relatively lower concentration of SA can elevate the mRNA and protein expressions of HO-1 and Nrf2 significantly within 1 mmol•L^-1 in HepG2 cells.

Key words: palmitic acid, stearic acid, HepG2 cells, heme oxygenase 1, nuclear transcription factor Nrf2

中图分类号:

S852.2

郝丽红，张秀英，张金铭，崔晓旭，孙晓琦，关舒函. 棕榈酸和硬脂酸对HepG2细胞血红素加氧酶-1和核转录因子Nrf2表达的影响[J]. 畜牧兽医学报, 2016, 47(3): 603-608.

HAO Li-hong，ZHANG Xiu-ying，ZHANG Jin-ming，CUI Xiao-xu，SUN Xiao-qi，GUAN Shu-han. Effects of Palmitic Acid and Stearic Acid on Genes and Protein Expressions of Heme Oxygenase 1 and Nuclear Transcription Factor Nrf2 in HepG2 Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 603-608.

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棕榈酸和硬脂酸对HepG2细胞血红素加氧酶-1和核转录因子Nrf2表达的影响

Effects of Palmitic Acid and Stearic Acid on Genes and Protein Expressions of Heme Oxygenase 1 and Nuclear Transcription Factor Nrf2 in HepG2 Cell

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