外源性dsRNA 对蛋鸡卵泡CART基因表达及雌激素和孕酮分泌的影响

doi:10.11843/j.issn.0366-6964.2016.03.013

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (3): 515-520.doi: 10.11843/j.issn.0366-6964.2016.03.013

外源性dsRNA 对蛋鸡卵泡CART基因表达及雌激素和孕酮分泌的影响

李鹏飞¹，孟金柱²，于雪静²，庞钰莹²，杜海燕²，李晓明²，姚晓磊²，赵妙妙²，吕丽华^2*

(1.山西农业大学生命科学学院，太谷 030801； 2.山西农业大学动物科技学院，太谷 030801)

收稿日期:2015-08-26 出版日期:2016-03-23 发布日期:2016-03-21
通讯作者: 吕丽华，E-mail：lihualvsxau@126.com
作者简介:李鹏飞（1978-），男，山西偏关人，博士，副教授，主要从事动物生殖生理方面的研究，E-mail：adamlpf@126.com
基金资助:
国家自然科学基金（31172211）；农业部948项目（2010-Z43）；山西省横向协作与委托项目（2010HX54）；山西省回国留学人员科研资助项目（2014-重点5）；山西省科技攻关项目（20130311027-2）；山西省人事厅人才引进项目；山西农业大学引进人才博士科研启动费(2014ZZ04) ；科研管理费资助重大项目和标志性成果培育项目（71060003）

Effects of Exogenous dsRNA on CART Gene Expression and Secretion of Estrodiol and Progestin in Layer Follicles

LI Peng-fei¹，MENG Jin-zhu²，YU Xue-jing²，PANG Yu-ying²，DU Hai-yan²，LI Xiao-ming²，YAO Xiao-lei²，ZHAO Miao-miao²，LÜ Li-hua^2*

（1.College of Life Science，Shanxi Agricultural University，Taigu 030801，China ；2.College of Animal Science and Technology，Shanxi Agricultural University，Taigu 030801，China）

Received:2015-08-26 Online:2016-03-23 Published:2016-03-21

摘要/Abstract

摘要：

旨在研究双链RNA（Double-stranded RNA，dsRNA）对海兰褐蛋鸡卵泡可卡因-苯丙胺调节转录肽（Cocaine-and amphetamine-regulated transcript，CART）基因表达及雌激素（Estrodiol，E₂）和孕酮（Progestin，P）分泌的影响。选择60只20周龄海兰褐蛋鸡，全价日粮预饲30 d后，随机平均分为3个处理组：对照组（A组）、dsRNA每5 d注射1次（B组）、dsRNA仅第1天注射1次（C组）；30 d后每组各选择10只健康蛋鸡宰杀，分别采集3~5、6~8、9~12 mm卵泡，提取总RNA进行qRT-PCR检测；并对E₂和P浓度进行测定。结果显示：注射dsRNA后，处理组B和C组CART表达量与对照组相比均降低，且在6~8 mm卵泡CART表达量显著低于对照组（P＜0.05）；B组注射dsRNA后，可显著提高6~8、9~12 mm卵泡E₂的分泌（P＜0.05）；但各处理组对各卵泡P浓度无显著影响。表明体外静脉注射dsRNA可抑制蛋鸡卵泡CART的表达，并进一步通过促进卵泡细胞E₂的分泌来影响卵泡的发育。

关键词: 蛋鸡, 卵泡, CART, 双链RNA, 雌激素, 孕酮

Abstract:

To investigate the effect of double-stranded RNA(dsRNA) on cocaine-and amphetamine-regulated transcript(CART) gene expression and estrodiol(E₂) and progestin(P) secretion in follicles of Highland Brown Laying hen.A total of 60 healthy layers at 20-week-old were selected and randomly divided into 3 experimental groups(A group：control group；B group：with treatment of dsRNA intravenous injection every 5 d；and C group：with treatment of dsRNA one initial injection).After 30 d of feeding，the different size of 3-5，6-8，9-12 mm follicles of 10 layers from each group were collected for analyzing mRNA expression of CART and determining the concentration of E₂ and P.The results showed that mRNA expression of CART was decreased in group B and C，compared with the control group.Especially，mRNA expression of CART in 6-8 mm follicle was lower than that in the control group with significant difference（P＜0.05）;The levels of E₂ was increased in the follicles with the size of 6-8 and 9-12 mm in group B with significant difference（P＜0.05）；However，there was no significant difference in the level of P among different treatment groups.The results suggested that intravenous injection of dsRNA could affect the follicular development through inhibiting the mRNA expression of CART and promoting the E₂ production in layer.

Key words: hen, follicle, CART, dsRNA, estrodiol, progestin

中图分类号:

S831.2

李鹏飞，孟金柱，于雪静，庞钰莹，杜海燕，李晓明，姚晓磊，赵妙妙，吕丽华. 外源性dsRNA 对蛋鸡卵泡CART基因表达及雌激素和孕酮分泌的影响[J]. 畜牧兽医学报, 2016, 47(3): 515-520.

LI Peng-fei，MENG Jin-zhu，YU Xue-jing，PANG Yu-ying，DU Hai-yan，LI Xiao-ming，YAO Xiao-lei，ZHAO Miao-miao，Lü Li-hua. Effects of Exogenous dsRNA on CART Gene Expression and Secretion of Estrodiol and Progestin in Layer Follicles[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 515-520.

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外源性dsRNA 对蛋鸡卵泡CART基因表达及雌激素和孕酮分泌的影响

Effects of Exogenous dsRNA on CART Gene Expression and Secretion of Estrodiol and Progestin in Layer Follicles

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