进口奶牛十二指肠贾第虫感染情况及其多位点基因序列研究

doi:10.11843/j.issn.0366-6964.2016.01.022

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (1): 165-171.doi: 10.11843/j.issn.0366-6964.2016.01.022

进口奶牛十二指肠贾第虫感染情况及其多位点基因序列研究

王臣荣，张振杰，李俊强，余复昌，曹建科，张龙现^*

(河南农业大学牧医工程学院，河南省人兽共患病国际联合实验室，郑州 450002)

收稿日期:2015-06-20 出版日期:2016-01-23 发布日期:2016-01-19
通讯作者: 张龙现，教授，E-mail：zhanglx8999@henau.edu.cn
作者简介:王臣荣(1985-)，男，河南南阳人，硕士生，主要从事人兽共患寄生虫学研究，E-mail：1203431811@qq.com
基金资助:
十二五国家重大科技专项课题(2012ZX10004220)；河南省科技创新杰出人才计划（134200510012）；河南省高校科研创新团队项目(012IRTSTHN005)

The Investigation of Infection and Multilocus Sequence of Giardia duodenalis in Dairy Cattle from an Imported Farm

WANG Chen-rong，ZHANG Zhen-jie，LI Jun-qiang，YU Fu-chang，CAO Jian-ke，ZHANG Long-xian^*

(College of Animal Science and Veterinary Medicine，Henan Agricultural University，International Joint Research Laboratory for Zoonotic Diseases of Henan Province，Zhengzhou 450002，China)

Received:2015-06-20 Online:2016-01-23 Published:2016-01-19

摘要/Abstract

摘要：

为了解进口奶牛十二指肠贾第虫感染情况及其集聚体和基因亚型分布特征，作者基于SSU rRNA、tpi、gdh和bg基因位点对奶牛十二指肠贾第虫进行PCR检测。结果如下：基于SSU rRNA基因位点进行PCR检测，奶牛十二指肠贾第虫感染率为9.5%(48/507)，48份阳性样品均为十二指肠贾第虫集聚体E；断奶后犊牛感染率最高(20.70%)，不同年龄段奶牛十二指肠贾第虫感染率统计学差异极显著 (P<0.01)。基于tpi、gdh和bg基因位点分别扩增出48份阳性样品中19、28和34个，1份在tpi基因位点呈集聚体A和E混合感染。多位点基因序列分析和种系进化树分析结果显示，该场进口奶牛应为引进后感染十二指肠贾第虫。研究表明进口成年奶牛不易携带人兽共患十二指肠贾第虫，引种场奶牛传播人兽共患十二指肠贾第虫风险较低。

关键词: 十二指肠贾第虫, 集聚体, 基因亚型, 奶牛

Abstract:

We investigated the infection of Giardia duodenalis in dairy cattle from an imported farm，and then we analyzed the distribution of the genotypes and assemblage of G.duodenalis.The detection of G.duodenalis in dairy cattle base on the locus of SSU rRNA，tpi，gdh and bg by PCR. Results indicated that the prevalence of G.duodenalis in dairy cattle is 9.8% (48/507) based on SSU rRNA by PCR，and 48 positive samples were identified assemblage E.The prevalence of G.duodenalis in post-weaning calves was the highest (20.70%)，the difference of the prevalence of G.duodenalis in cattle in different ages was significant.The 48 positive samples were amplified 19，28 and 34 base on tpi，gdh and bg locus respectively，one sample was mixed infection (assemblage A and assemblage E).Multilocus sequence and phylogenetic analysis indicated that G.duodenalis infecting dairy cattle from an imported farm may be from the local area. The results indicate that imported adult dairy cattle is not ease to carry Zoonotic G.duodenalis，dairy cattle from an imported farm have a lower risk to transmite Zoonotic G.duodenalis.

Key words: Giardia duodenalis, assemble, genotypes, dairy cattle

中图分类号:

S855.99

王臣荣，张振杰，李俊强，余复昌，曹建科，张龙现. 进口奶牛十二指肠贾第虫感染情况及其多位点基因序列研究[J]. 畜牧兽医学报, 2016, 47(1): 165-171.

WANG Chen-rong，ZHANG Zhen-jie，LI Jun-qiang，YU Fu-chang，CAO Jian-ke，ZHANG Long-xian. The Investigation of Infection and Multilocus Sequence of Giardia duodenalis in Dairy Cattle from an Imported Farm[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 165-171.

参考文献

［1］RYAN U，CACCIÒ S M.Zoonotic potential of Giardia ［J］.Int J Parasitol，2013，43(12-13)：943-956.
［2］PENROSE A S，WELLS E V，AIELLO A E.Infectious causation of chronic disease：Examining the relationship between Giardia lamblia infection and irritable bowel syndrome［J］.World J Gastroenterol，2007，13(34)：4574-4578.
［3］GEURDEN T，VERCRUYSSE J，CLAEREBOUT E.Is Giardia a significant pathogen in production animals?［J］.Exp Parasitol，2010，124(1)：98-106.
［4］THOMPSON R C.The zoonotic significance and molecular epidemiology of Giardia and giardiasis［J］.Vet Parasitol，2004，126(1-2)：15-35.
［5］FORONDA P，BARGUES M D，ABREU-ACOSTA N，et al.Identification of genotypes of Giardia intestinalis of human isolates in Egypt［J］.Parasitol Res，2008，103(5)：1177-1181.
［6］WANG H，ZHAO G，CHEN G，et al.Multilocus genotyping of Giardia duodenalis in dairy cattle in Henan，China［J］.PLoS One，2014，9(6)：e100453.
［7］LIU A，ZHANG X，ZHANG L，et al.Occurrence of bovine giardiasis and endemic genetic characterization of Giardia duodenalis isolates in Heilongjiang Province，in the Northeast of China［J］.Parasitol Res，2012，111(2)：655-661.
［8］APPELBEE A J，FREDERICK L M，HEITMAN T L，et al.Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta，Canada［J］.Vet Parasitol，2003，112(4)：289-294.
［9］SULAIMAN I M，FAYER R，BERN C，et al.Triosephosphate isomerase gene characterization and potential zoonotic t ransmission of Giardia duodenalis ［J］.Emerg Infect Dis，2003，9(11)：1444-1452.
［10］CACCIÒ S M，BECK R，LALLE M，et al.Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B［J］.Int J Parasitol，2008，38(13)：1523-1531.
［11］LALLE M，POZIO E，CAPELLI G，et al.Genetic heterogeneity at the beta-giardin locus among human and animal isolates of Giardiaduodenalis and identification of potentially zoonotic subgenotypes［J］.Int J Parasitol，2005，35(2)：207-213.
［12］张静，苏艳，白光彦，等.东北地区部分犊牛感染贾第虫的基因型及基因亚型分析［J］.中国兽医学报，2012，32(11)：1679-1682，1707.
ZHANG J，SU Y，BAI G Y，et al.Genotype in dentification of Giardia from cattle in part areas of northeast China［J］.Chinese Journal of Veterinary Science，2012，32(11)：1679-1682，1707.(in Chinese)
［13］HUANG J，YUE D，QI M，et al.Prevalence and molecular characterization of Cryptosporidium spp.and Giardia duodenalis in dairy cattle in Ningxia，northwestern China［J］.BMC Vet Res，2014，10：292.
［14］NG J，YANG R，McCARTHY S，et al.Molecular characterization of Cryptosporidium and Giardia in pre-weaned calves in Western Australia and New South Wales［J］.Vet Parasitol，2011，176(2-3)：145-150.
［15］BECHER K A，ROBERTSON I D，FRASER D M，et al.Molecular epidemiology of Giardia and Cryptosporidium infections in dairy calves originating from three sources in Western Australia［J］.Vet Parasitol，2004，123(1-2)：1-9.
［16］王海燕，齐萌，李爱心，等.开封地区奶牛肠道寄生虫感染情况调查［J］.畜牧与兽医，2014，46(12)：84-87.
WANG H Y，QI M，LI A X，et al.Investigation of parasite infection in dairy cattle in Kaifeng［J］. Animal Husbandry and Veterinary Medicine，2014，46(12)：84-87.(in Chinese)
［17］张小云.黑龙江省部分地区牛贾第虫的感染情况及分离株的分子鉴定［D］.哈尔滨：哈尔滨医科大学，2012.
ZHANG X Y.Prevalence of Giardia duodenalis and molecular identification of isolates in cattle in partial areas of Heilongjiang Province［D］.Harbin：Harbin Medical University，2012.(in Chinese)
［18］O’HANDLEY R M，COCKWILL C，McALLISTER T A，et al.Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhea［J］.J Am Vet Med Assoc，1999，214(3)：391-396.
［19］MARK-CAREW M P，WADE S E，CHANG Y F，et al.Prevalence of Giardia duodenalis assemblages among dairy herds in the New York City Watershed［J］.Vet Parasitol，2012，185(2-4)：151-157.

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进口奶牛十二指肠贾第虫感染情况及其多位点基因序列研究

The Investigation of Infection and Multilocus Sequence of Giardia duodenalis in Dairy Cattle from an Imported Farm

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