转录因子USF1调控鸡小肠上皮细胞中糖类转运蛋白表达

doi:10.11843/j.issn.0366-6964.2015.10.003

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (10): 1713-1720.doi: 10.11843/j.issn.0366-6964.2015.10.003

转录因子USF1调控鸡小肠上皮细胞中糖类转运蛋白表达

张利环¹，李玲香²，张瑜²，原一桐¹，王文魁²，朱芷葳¹，王钦德²，李慧锋^1*

（1.山西农业大学生命科学学院，太谷 030801； 2.山西农业大学动物科技学院，太谷 030801）

收稿日期:2015-04-14 出版日期:2015-10-23 发布日期:2015-10-19
通讯作者: 李慧锋，博士，副教授，硕士生导师，Tel：0354-6285908，E-mail：lihuifengtom@163.com
作者简介:张利环（1970-），女，山西太谷人，副教授，硕士生导师，主要从事家禽遗传育种和营养的分子生物学研究，E-mail：tgzhanglh@163.com
基金资助:
国家自然科学基金（31172203）；山西省青年科技研究基金（2011021028-1）；山西省科技攻关项目基金（20140311021-4）

Transcription Factor USF1 Regulate the Expression of Monosaccharide Transporters in Chicken Intestinal Epithelial Cells

ZHANG Li-huan¹，LI Ling-xiang²，ZHANG Yu²，YUAN Yi-tong¹，WANG Wen-kui²，ZHU Zhi-wei¹，WANG Qin-de²，LI Hui-feng^1*

(1.College of Life Science，Shanxi Agricultural University，Taigu 030801，China；2.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China)

Received:2015-04-14 Online:2015-10-23 Published:2015-10-19

摘要/Abstract

摘要：

为了研究转录因子USF1在鸡小肠上皮细胞中调控糖转运蛋白基因表达的机制，本试验在生物信息学分析的基础上通过构建真核表达载体pcDNA3.1-USF1，在鸡小肠上皮细胞中过表达转录因子USF1，用绝对荧光定量PCR的方法检测分析了USF1对SGLT1、GLUT2和GLUT5基因表达的影响。结果表明，受到USF1基因过表达的影响，糖类转运蛋白GLUT2和SGLT1 mRNA在鸡小肠上皮细胞中的表达量极显著增高（P＜0.01）；而GLUT5 mRNA表达量没有显著变化（P＞0.05）。结合本研究结果，经过对这3个糖类转运蛋白基因5′上游调控区USF1结合位点的深入分析，认为在鸡小肠上皮细胞中转录因子USF1对糖转运蛋白基因GLUT2和SGLT1的表达具有正向调控的作用。

关键词: USF1, 糖类转运蛋白, 过表达, 小肠上皮细胞

Abstract:

In order to study the potential regulation mechanism of the USF1 on monosaccharide transporters in chicken intestinal epithelial cells，we built the eukaryote expression vector pcDNA3.1-USF1 on the basis of bioinformatics analysis and made the transcription factor USF1 over-expressed in chicken intestinal epithelial cells.Besides，we detected and analyzed the influence of USF1 on the mRNA expressions of SGLT1，GLUT2 and GLUT5 by Real-time PCR.The results indicated that the over-expression of USF1 gene significantly up-regulated the mRNA expressions of GLUT2 and SGLT1 in chicken intestinal epithelial cells(P＜0.01)，while the expression of GLUT5 were not changed significantly(P＞0.05).Combining the results with the bioinformatics analysis of the USF1 binding sites on the 5′up-stream regulation area of the 3 monosaccharide transporters，our results indicated that the transcription factor USF1 positively regulated the expression of SGLT1 and GLUT2 in chicken intestinal epithelial cells.

Key words: USF1, monosaccharide transporter, over-expression, intestinal epithelial cells

中图分类号:

S831.2

张利环，李玲香，张瑜，原一桐，王文魁，朱芷葳，王钦德，李慧锋. 转录因子USF1调控鸡小肠上皮细胞中糖类转运蛋白表达[J]. 畜牧兽医学报, 2015, 46(10): 1713-1720.

ZHANG Li-huan，LI Ling-xiang，ZHANG Yu，YUAN Yi-tong，WANG Wen-kui，ZHU Zhi-wei，WANG Qin-de，LI Hui-feng. Transcription Factor USF1 Regulate the Expression of Monosaccharide Transporters in Chicken Intestinal Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1713-1720.

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转录因子USF1调控鸡小肠上皮细胞中糖类转运蛋白表达

Transcription Factor USF1 Regulate the Expression of Monosaccharide Transporters in Chicken Intestinal Epithelial Cells

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