版纳微型猪近交系DLDH基因编码区序列扩增及生物信息学分析

doi:10.11843/j.issn.0366-6964.2015.10.002

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (10): 1706-1712.doi: 10.11843/j.issn.0366-6964.2015.10.002

版纳微型猪近交系DLDH基因编码区序列扩增及生物信息学分析

成文敏^1#，潘伟荣^1#，尹俊芳²，黄言³，查星琴¹，肖晶³，曾养志^3*

（1.云南农业大学动物科学技术学院，昆明 650201；2.昆明市官渡区人民医院，昆明 650021；3.云南省版纳微型猪近交系重点实验室，昆明 650201）

收稿日期:2014-11-17 出版日期:2015-10-23 发布日期:2015-10-19
通讯作者: 曾养志，教授，E-mail:bnwxzjjx@yahoo.com.cn
作者简介:成文敏（1980-），女，山西交城人，博士，副教授，主要从事动物胚胎生物技术研究，E-mail：cheng_8097@163.com；潘伟荣（1973-），男，云南宁蒗人，实验师，硕士，主要从事动物遗传育种研究，E-mail：pwr2000@sina.com。成文敏和潘伟荣并列为第一作者

Amplification and Bioinformatics Analysis of Coding Region of the DLDH Gene in Banna Mini-pig Inbred Line (BMI)

CHENG Wen-min^1# ，PAN Wei-rong^1# ，YIN Jun-fang²，HUANG Yan³，ZHA Xing-qin¹，XIAO Jing³，ZENG Yang-zhi^3*

(1.College of Animal Science and Technology，Yunnan Agricultural University，Kunming 650201，China；2.Guandu District People’s Hospital of Kunming City，Kunming 650021，China；3.Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province，Kunming 650201，China)

Received:2014-11-17 Online:2015-10-23 Published:2015-10-19

摘要/Abstract

摘要：

本试验旨在扩增版纳微型猪近交系DLDH基因编码区序列，为进一步研究DLDH基因的表达信号通路及与精子获能和顶体反应发生相关基因的表达调控提供必要的基础。通过提取版纳微型猪近交系睾丸组织RNA，经反转录后获得cDNA，设计特异性引物以扩增DLDH基因的编码区序列，经测序后采用生物信息学软件对编码区序列及氨基酸序列进行生物信息学分析和结构预测。结果表明，版纳微型猪近交系DLDH基因编码区序列全长1 530 bp，编码509个氨基酸。生物信息学分析表明，不同物种DLDH基因编码区核苷酸序列和氨基酸序列具有较强的保守性，DLDH蛋白中存在丰富的磷酸化位点，空间结构以α-螺旋和无规则卷曲为主。

关键词: 版纳微型猪近交系, DLDH基因, 编码区, 蛋白质结构预测

Abstract:

The study aimed to amplify the coding region of DLDH gene，which could be used to further study the signal pathway of DLDH expression and the correlation between DLDH gene expression and sperm capacitation，acrosome reaction.RNA was extracted from testicle and cDNA was obtained by reverse transcription.Specific primers were designed to amplify the coding region of DLDH gene.The sequence of coding region and amino acid of DLDH were analyzed and predicted by Lasergene，BioEdit，ClustalX，Mega bio-softs，and the molecular phylogenetic trees of coding amino acids region of DLDH gene was constructed by NJ and ME，respectively.The sequence length of coding region of the BMI DLDH gene was 1 530 bp，encoding a protein of 509 amino acids.By biology software analysis，the nucleotide and amino acid sequences of DLDH among different species were conserved in evolution.The DLDH protein contained the abundant phosphorylated sites and was assembled with α-helixes and random coil.

Key words: BMI, DLDH gene, coding region, protein structure prediction

中图分类号:

S814

成文敏，潘伟荣，尹俊芳，黄言，查星琴，肖晶，曾养志. 版纳微型猪近交系DLDH基因编码区序列扩增及生物信息学分析[J]. 畜牧兽医学报, 2015, 46(10): 1706-1712.

CHENG Wen-min，PAN Wei-rong，YIN Jun-fang，HUANG Yan，ZHA Xing-qin，XIAO Jing，ZENG Yang-zhi. Amplification and Bioinformatics Analysis of Coding Region of the DLDH Gene in Banna Mini-pig Inbred Line (BMI)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1706-1712.

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版纳微型猪近交系DLDH基因编码区序列扩增及生物信息学分析

Amplification and Bioinformatics Analysis of Coding Region of the DLDH Gene in Banna Mini-pig Inbred Line (BMI)

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