山羊y+L碱性氨基酸转运系统转录水平表达的研究

doi:10.11843/j.issn.0366-6964.2015.08.005

摘要/Abstract

摘要：

旨在研究山羊y⁺L碱性氨基酸转运系统中4F2hc、y⁺LAT1和y⁺LAT2 mRNA表达的组织特异性及其在不同年龄山羊小肠中的发育性表达规律。试验选取健康、体重相近的1日龄、6月龄、8月龄、10月龄、12月龄陕北白绒山羊各3只，共15只，屠宰后采集心、肝、肾、大脑、背最长肌、体侧皮肤、瘤胃、十二指肠、空肠、回肠和结肠组织，利用实时荧光定量PCR技术检测4F2hc、y⁺LAT1和y⁺LAT2 mRNA在1日龄山羊上述组织和全部山羊十二指肠、空肠和回肠的表达情况。结果表明，（1）4F2hc、y⁺LAT1和y⁺LAT2 mRNA在1日龄山羊多种组织中均有表达，且有一定的组织特异性。其中，4F2hc mRNA在皮肤表达量最高，y⁺LAT1 mRNA在回肠表达量最高，y⁺LAT2 mRNA在大脑和回肠表达量最高。（2）4F2hc mRNA在山羊十二指肠、空肠和回肠表达量均以1日龄山羊为最高，其他月龄山羊相同肠段之间表达量无显著差异（P＞0.05）；y⁺LAT1 mRNA在十二指肠、空肠和回肠表达量随山羊年龄的增加均呈现逐渐降低的趋势；y⁺LAT2 mRNA在十二指肠、空肠和回肠表达量随山羊年龄的增加呈现先升高后降低的趋势，其在10月龄山羊表达量最高；y⁺LAT2 mRNA在回肠表达量以1日龄山羊为最低，其他月龄山羊之间差异不显著（P＞0.05）。结果说明，山羊y⁺L碱性氨基酸转运系统的主要转运部位为小肠；该系统中轻链y⁺LAT1和重链4F2hc构成的异二聚体转运蛋白可能发挥更为主要的转运作用；4F2hc和y+LAT2基因可能分别在山羊皮肤和大脑中发挥重要的生理功能；4F2hc、y⁺LAT1和y⁺LAT2基因在山羊小肠中受肠段和发育阶段的影响，具有不同的发育性表达规律。

关键词: 山羊, y⁺L碱性氨基酸转运系统, 实时荧光定量PCR, mRNA表达

Abstract:

The objective of this study was to research the mRNA expression of goat 4F2hc，y⁺LAT1 and y⁺LAT2 (y⁺L cationic amino acid transporter system) in various tissues and their developmental expression in small intestine of goats at different ages.A total of 15 Shanbei White Cashmere goats at the ages of day 1，month 6，8，10 and 12 (3 goats at each age) were slaughtered to collect heart，liver，kidney，brain，muscle，skin，rumen，duodenum，jejunum，ileum and colon tissues.Then 4F2hc，y⁺LAT1 and y⁺LAT2 mRNA were quantified by Real-time quantitative PCR in all collected tissues of 1-day-old goats and in duodenum，jejunum，ileum of all goats.The results indicated that，(1) 4F2hc，y⁺LAT1 and y⁺LAT2 mRNA were widely expressed in all collected tissues of 1-day-old goats and the expression level varied from tissues.Specifically，4F2hc mRNA in skin，y⁺LAT1 mRNA in ileum and y⁺LAT2 mRNA in brain and ileum had the highest abundance.(2) 4F2hc mRNA of duodenum，jejunum and ileum on 1-day-old goats had higher abundance than that in goats on the other month-old，and there was no significant difference in the same segment of small intestine in goats on the other month-old (P>0.05)；y⁺LAT1 mRNA abundance in small intestine of goats decreased with age；y⁺LAT2 mRNA abundance in small intestine peaked in 10-month-old goats and then dropped with age，while it in ileum of 1-day-old goats had lower abundance than that in goats on the other month-old，and there was no significant difference in ileum between goats on the other month-old (P>0.05).In conclusion，y⁺L cationic amino acid transporter system played a main role in transferring amino acid in small intestine of goats and the heterodimer consisting of y⁺LAT1 and 4F2hc might be the main transporter protein in y⁺L system.4F2hc and y⁺LAT2 might perform important physiological functions in skin and brain of goats，respectively. 4F2hc，y⁺LAT1 and y⁺LAT2 were differentially regulated and distributed with the differences of developmental stages and segments of small intestine in goat.

Key words: goat, y⁺L cationic amino acid transporter system, Real-time quantitative PCR, mRNA expression

中图分类号:

S827.2

周英昊，高晔，刘林丽，曾洁，杨雨鑫，张恩平，陈玉林. 山羊y⁺L碱性氨基酸转运系统转录水平表达的研究[J]. 畜牧兽医学报, 2015, 46(8): 1308-1316.

ZHOU Ying-hao，GAO Ye，LIU Lin-li，ZENG Jie，YANG Yu-xin，ZHANG En-ping，CHEN Yu-lin. Study on Transcriptional Level of y⁺L Cationic Amino Acid Transporter System in Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1308-1316.

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