麦冬多糖脂质体增强雏鸡免疫功能的分析

doi:10.11843/j.issn.0366-6964.2015.07.023

摘要/Abstract

摘要：

为探讨麦冬多糖脂质体（OPL）的增强免疫活性，进行以下试验。体外试验，采用MTT法测定OPL对雏鸡脾中T、B淋巴细胞增殖的影响，并以麦冬多糖（OP）作为对照；体内试验，400只11日龄雏鸡随机均分为8组，先用环磷酰胺进行免疫抑制造模，14日龄时用新城疫疫苗进行免疫，在免疫的同时注射不同剂量的OPL，然后测定免疫后不同时间点OPL对外周血淋巴细胞增殖、抗体效价和细胞因子含量的影响。结果显示，在体外，OPL在7.813~125 μg•mL^-1时无论是单独作用还是协同PHA、LPS作用均能显著促进鸡脾淋巴细胞的增殖，且效果显著优于OP（P<0.05）；在体内，OPL高、中剂量能够显著促进淋巴细胞增殖，增强抗体效价，提高细胞因子IL-2和IFN-γ的含量，且效果显著优于OP（P<0.05）。结果表明，OPL能够显著提高OP的增强免疫活性，有望开发成新型的麦冬多糖制剂。

关键词: 麦冬多糖脂质体, 免疫功能, 淋巴细胞增殖, 免疫抑制

Abstract:

In order to investigate the immune-enhancing activity of ophiopogon polysaccharide liposome (OPL)，two experiments were carried out.In vitro，the effects of OPL on splenocyte proliferation of chicken were determined by MTT method，and ophiopogon polysaccharide (OP) was used as control.In vivo，four hundred 11-day-old chickens were randomly assigned into 8 groups，and the chickens were injected with cyclophosphamide to build the immunosuppression model.At 14-day-old，the chickens were vaccinated with Newcastle disease vaccine.At the same time of the first vaccination，the chickens were injected respectively with OPL at different dosages.At various time points after the first vaccination，the peripheral lymphocyte proliferation，serum antibody titer and the contents of cytokines were measured.The results showed that OPL could significantly promote splenocyte proliferation singly or synergistically with PHA and LPS at 7.813-125 μg•mL^-1 in vitro，and the efficacy was superior to OP (P<0.05)；In vivo，OPL at high and medium doses could significantly promote lymphocyte proliferation，enhance antibody titer and improve the contents of IL-2 and IFN-γ compared with OP at most time points (P<0.05).These results indicated that OPL could significantly improve the immune-enhancing activity of OP，OPL would be expected to exploit into a new-type preparation of OP.

Key words: ophiopogon polysaccharide liposome, immune function, lymphocyte proliferation, immunosuppression

中图分类号:

S853.74

范云鹏，马琳，张为民，宋晓平，郭超，侯伟峰，童德文. 麦冬多糖脂质体增强雏鸡免疫功能的分析[J]. 畜牧兽医学报, 2015, 46(7): 1246-1252.

FAN Yun-peng，MA Lin，ZHANG Wei-min，SONG Xiao-ping，GUO Chao，HOU Wei-feng，TONG De-wen. Analysis of Ophiopogon Polysaccharide Liposome on Enhancing the Immune Function in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1246-1252.

参考文献

［1］XIONG S L，LI A L，HUANG N，et al.Antioxidant and immunoregulatory activity of different polysaccharide fractions from tuber of Ophiopogon japonicus［J］.Carbohyd Polym，2011，86(3)：1273-1280.
［2］WANG X M，SUN R G，ZHANG J，et al.Structure and antioxidant activity of polysaccharide POJ-U1a extracted by ultrasound from Ophiopogon japonicus［J］.Fitoterapia，2012，83(8)：1576-1584.
［3］LIN X，WANG S，JIANG Y，et al.Poly (ethylene glycol)-radix Ophiopogonis polysaccharide conjugates：preparation，characterization，pharmacokinetics and in vitro bioactivity［J］.Eur J Pharm Biopharm，2010，76(2)：230-237.
［4］PATIL Y P，JADHAV S.Novel methods for liposome preparation［J］.Chem Phys Lipids，2014，177：8-18.
［5］YU Y，LU Y，BO R N，et al.The preparation of gypenosides liposomes and its effects on the peritoneal macrophages function in vitro［J］.Int J Pharm，2014，460(1-2)：248-254.
［6］ZHAO X J，FAN Y P，WANG D Y，et al.Immunological adjuvant efficacy of glycyrrhetinic acid liposome against Newcastle disease vaccine［J］.Vaccine，2011，29(52)：9611-9617.
［7］YUAN J，LIU J G，HU Y L，et al.The immunological activity of propolis flavonoids liposome on the immune response against ND vaccine［J］. Int J Biol Macromol，2012，51(4)：400-405.
［8］FAN Y，SONG X，GAO Y，et al.Preparation and optimization of ophiopogon polysaccharide liposome and its activity on Kupffer cells［J］.Int J Pharm，2014，477(1-2)：421-430.
［9］THEKISOE M M，MBATI P A，BISSCHOP S P.Different approaches to the vaccination of free ranging village chickens against Newcastle disease in Qwa-Qwa，South Africa［J］.Vet Microbiol，2004，101(1)：23-30.
［10］EL-ABASY M，MOTOBU M，NAKAMURA K，et al.Preventive and therapeutic effects of sugar cane extract on cyclophosphamide-induced immunosuppression in chickens［J］. Int Immunopharmacol，2004，4(8)：983-990.
［11］CRUZ-CHAMORRO L，PUERTOLLANO M A，PUERTOLLANO E，et al.Examination of host immune resistance against Listeria monocytogenes infection in cyclophosphamide-treated mice after dietary lipid administration［J］.Clin Nutr，2007，26(5)：631-639.
［12］ATSAMO A D，NGUELEFACK T B，DATTÉ J Y，et al.Acute and subchronic oral toxicity assessment of the aqueous extract from the stem bark of Erythrina senegalensis DC (Fabaceae) in rodents［J］.J Ethnopharmacol，2011，134(3)：697-702.
［13］MINATO K，KAWAKAMI S，NOMURA K，et al.An exo β-1，3-glucanase synthesized de novo degrades lentinan during storage of Lentinule edodes and diminishes immunomodulating activity of the mushroom［J］.Carbohyd Polym，2004，56(3)：279-286.
［14］HUANG X Y，HU Y L，ZHAO X N，et al．Sulfated modification can enhance the adjuvant activity of astragalus polysaccharide for ND vaccine［J］.Carbohyd polym，2008，73(2)：303-308．
［15］HUANG Y，WU C，LIU Z，et al.Optimization on preparation conditions of Rehmannia glutinosa polysaccharide liposome and its immunological activity［J］.Carbohydr Polym，2014，104：118-126.
［16］YU Y，WANG D Q，ABULA S，et al.The immunological adjuvant activity of gypenosides liposome against Newcastle disease vaccine［J］.Int J Biol Macromol，2013，60：116-121.
［17］PARRA F，PRIETO M.Purification and characterization of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits［J］.J Virol，1990，64(8)：4013-4015.
［18］邱妍，胡元亮，崔保安，等.怀牛膝多糖对雏鸡疫苗免疫效果的影响［J］.畜牧兽医学报，2007，38(7)：723-727.
QIU Y，HU Y L，CUI B A，et al.Effects of achyranthes bidentata polysaccharide on immune efficacy of vaccine in chickens［J］.Acta Veterinaria et Zootechnica Sinica，2007，38(7)：723-727.（in Chinese）
［19］UNG C Y，LI H，KONG C Y，et al.Usefulness of traditionally defined herbal properties for distinguishing prescriptions of traditional Chinese medicine from non-prescription recipes［J］.J Ethnopharmacol，2007，109(1)：21-28.
［20］FAN Y P，HU Y L，WANG D Y，et al.Effects of astragalus polysaccharide liposome on lymphocyte proliferation in vitro and adjuvanticity in vivo［J］.Carbohyd Polym，2012，88(1)：68-74.
［21］FAN Y P，MA L，ZHANG W M，et al.Liposome can improve the adjuvanticity of astragalus polysaccharide on the immune response against ovalbumin［J］.Int J Biol Macromol，2013，60：206-212.
［22］NHIEM N X，KIEM P V，MINH C V，et al.Structure-activity relationship of lupane-triterpene glycosides from Acanthopanax koreanum on spleen lymphocyte IL-2 and IFN-γ［J］. Bioorg Med Chem Lett，2010，20 (16)：4927-4931.
［23］LETSCH A，SCHEIBENBOGEN C.Quantification and characterization of specific T-cells by antigen-specific cytokine production using ELISPOT assay or intracellular cytokine staining［J］.Methods，2003，31(2)：143-149.

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