单色光及松果体摘除对肉鸡肝抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2014.11.020

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1883-1887.doi: 10.11843/j.issn.0366-6964.2014.11.020

单色光及松果体摘除对肉鸡肝抗氧化功能的影响

李素琪，陈耀星^*，王子旭，曹静，李芳，曲喆，黄颖，庞茉

（中国农业大学动物医学院，北京 100193）

收稿日期:2014-04-02 出版日期:2014-11-23 发布日期:2014-11-24
通讯作者: 陈耀星，教授，E-mail：yxchen@cau.edu.cn
作者简介:李素琪（1989-），女，湖南人，硕士生，主要从事神经生物学研究，E-mail：lscute@163.com
基金资助:
公益性行业（农业）科研专项（201303119）；国家自然科学基金（31172277；31272516；31372387）；高等学校博士科学点专项科研基金（20130008110031；20100008120004）；北京市大学生创新计划（2012bj035）

Effect of Monochromatic Light and Pinealectomy on Antioxidant Function of Liver in Broilers

LI Su-qi，CHEN Yao-xing^*，WANG Zi-xu，CAO Jing，LI Fang，QU Zhe，HUANG Ying，PANG Mo

(College of Animal Medicine，China Agricultural University，Beijing 100193，China)

Received:2014-04-02 Online:2014-11-23 Published:2014-11-24

摘要/Abstract

摘要：

本研究旨在探究单色光对肉鸡肝抗氧化功能的影响和褪黑激素的介导作用。选择120只0日龄AA肉鸡随机分为4组，分别饲养于白、红、绿和蓝光下（n=30），3日龄时按光色条件每组随机选取20只鸡分别施行松果体摘除手术（n=10）和假手术（n=10），剩余鸡为对照组，每组同条件饲养至14日龄时利用抗氧化试剂盒检测各光色不同处理组肉鸡肝抗氧化水平。结果显示，饲养于绿光下对照组和假手术组的肉鸡肝T-SOD、GSH-Px、T-AOC的水平显著高于其他光组（7.82%~46.33%，P<0.05），而MDA含量最低；松果体摘除阻断褪黑激素分泌，各处理组肉鸡肝抗氧化酶活性均降低（12.64%~38.50%），而MDA含量升高。以上结果说明，单色光能够影响肉鸡肝的抗氧化功能，其中绿光显著增强肝抗氧化功能；而摘除松果体后不同光色环境下的肝抗氧化能力减弱显著。提示褪黑激素参与了单色光影响肉鸡肝的抗氧化功能。

关键词: 单色光, 松果体摘除, 肝, 抗氧化, 肉鸡

Abstract:

The present study was aimed to explore the effect of monochromatic light on antioxidant function in liver and the role of melatonin in broilers.One hundred and twenty post-hatching day (P0) male Arbor Acres (AA) broilers were divided into four groups randomly and reared under the white，red，green and blue light environments (n=30).Twenty broilers from each light group was chosen randomly for pinealectomy surgery (n=10) and sham operation (n=10) at P3，and the remaining broilers were as the control group.Antioxidant assay kits were used to detect the antioxidant level in liver of broilers in different light groups reared under same environment to P14.The results showed that the T-SOD，GSH-Px，T-AOC level in liver of broilers reared under the green light in control and sham-operation groups were higher (7.82%-46.33%，P<0.05) than other various light groups，while the content of MDA was the lowest.After pinealectomy blocked the secretion of melatonin，the activity of antioxidant enzymes were reduced (12.64%-38.50%) and MDA contents were increased in various light groups.These results showed that the monochromatic light could influence the antioxidant level of liver in broilers，and green light enhanced the antioxidant function of liver.However，pinealectomy could significantly reduce the liver antioxidant level in various light groups.These results suggested that melatonin was involved in the effect of monochromatic light on antioxidant function in liver of broilers.

Key words: monochromatic light, pinealectomy, liver, antioxidant function, broiler

中图分类号:

S813.3

李素琪，陈耀星，王子旭，曹静，李芳，曲喆，黄颖，庞茉. 单色光及松果体摘除对肉鸡肝抗氧化功能的影响[J]. 畜牧兽医学报, 2014, 45(11): 1883-1887.

LI Su-qi，CHEN Yao-xing，WANG Zi-xu，CAO Jing，LI Fang，QU Zhe，HUANG Ying，PANG Mo. Effect of Monochromatic Light and Pinealectomy on Antioxidant Function of Liver in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1883-1887.

参考文献

［1］CAO J，LIU W，WANG Z，et al.Green and blue monochromatic lights promote growth and development of broilers via stimulating testosterone secretion and microfiber growth ［J］.J Appl Poult Res，2008，17(2)：211-218.
［2］XIE D，WANG Z，DONG Y L，et al.Effects of monochromatic light on immune response of broilers ［J］.Poult Sci，2008，87(8)：1535-1539.
［3］KÜHN E R，DARRAS V M，GYSEMANS C，et al.The use of intermittent lighting in broiler raising.2.Effects on the somatotrophic and thyroid axes and on plasma testosterone levels ［J］.Poult Sci，1996，75(5)：595-600.
［4］TUREKA F W，GILLETTEB M U.Melatonin，sleep，and circadian rhythms：rationale for development of specific melatonin agonists ［J］.Sleep Med，2004，5(6)：523-532.
［5］CASTRILLÓN P O，ESQUIFINO A I，VARAS A，et al.Effect of melatonin treatment on 24-h variations in responses to mitogens and lymphocyte subset populations in rat submaxillary lymph nodes ［J］.J Neuroendocrinol, 2000，12(8)：758-765.
［6］MAESTRONI G J.The immunotherapeutic potential of melatonin ［J］.Expert Opin Investig Drugs，2001，10(3)：467-476.
［7］LIU W J，WANG Z，CHEN Y.Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch period ［J］.Anat Rec (Hoboken)，2010，293(8)：1315-1324.
［8］SENER G，SEHIRLI A O，AYANOGˇLU-DÜLGER G.Protective effects of melatonin，vitamin E and N-acetylcysteine against acetaminophen toxicity in mice：a comparative study ［J］.J Pineal Res，2003，35(1)：61-68.
［9］CARRILLO-VICO A，LARDONE P J，NAJI L，et al.Beneficial pleiotropic actions of melatonin in an experimental model of septic shock in mice：regulation of pro-/anti-inflammatory cytokine network，protection against oxidative damage and anti-apoptotic effects ［J］.J Pineal Res，2005，39(4)：400-408.
［10］RODRIGUEZ C，MAYO J C，SAINZ R M，et al.Regulation of antioxidant enzymes：a significant role for melatonin ［J］.J Pineal Res，2004，36(1)：1-9.
［11］MEKI A R，ESMAIL EEL-D，HUSSEIN A A，et al.Caspase-3 and heat shock protein-70 in rat liver treated with aflatoxin B1：effect of melatonin ［J］.Toxicon，2004，43(1)：93-100.
［12］张军，胡大海.褪黑激素抗氧化作用的研究进展［J］.国际药学研究杂志.2008，35(3)：189-193.
［13］何炜，李晓晔，石鑫，等.褪黑素的生理活性研究进展［J］.医药导报，2006，25(6)：556-558.
［14］LIANG R，NICKKHOLGH A，HOFFMANN K，et al.Melatonin protects from hepatic reperfusion injury through inhibition of IKK and JNK pathways and modification of cell proliferation ［J］.J Pineal Res, 2009，46(1)：8-14.
［15］SEWERYNEK E，MELCHIORRI D，REITER R J，et al.Lipopolysaccharide-induced hepatotoxicity is inhibited by the antioxidant melatonin ［J］.Eur J Pharmacol，1995，293(4):327-334.
［16］SEWERYNEK E，REITER R J，MELCHIORRI D，et al.Oxidative damage in the liver induced by ischemia-reperfusion：protection by melatonin ［J］.Hepatogastroenterology，1996，43(10)：898-905.
［17］LIU F，NG T B.Effect of pineal indoles on activities of the antioxidant defense enzymes superoxide dismutase，catalase，and glutathione reductase，and levels of reduced and oxidized glutathione in rat tissues ［J］.Biochem Cell Biol, 2000，78(4):447-453.
［18］ALBARRÁN M T，LÓPEZ-BURILLO S，PABLOS M I，et al.Endogenous rhythms of melatonin，total antioxidant status and superoxide dismutase activity in several tissues of chick and their inhibition by light ［J］. J Pineal Res，2001，30(4)：227-233.
［19］BAYDAS G，GURSU M F，YILMAZ S，et al.Daily rhythm of glutathione peroxidase activity，lipid peroxidation and glutathione levels in tissues of pinealectomized rats ［J］.Neurosci Lett，2002，323(3)：195-198.
［20］HARDELAND R.Antioxidative protection by melatonin：multiplicity of mechanisms from radical detoxification to radical avoidance ［J］.Endocrine，2005，27(2)：119-130.

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单色光及松果体摘除对肉鸡肝抗氧化功能的影响

Effect of Monochromatic Light and Pinealectomy on Antioxidant Function of Liver in Broilers

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