禽戊型肝炎病毒组织切片免疫荧光检测方法的建立及应用

doi:10.11843/j.issn.0366-6964.2014.11.016

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1851-1857.doi: 10.11843/j.issn.0366-6964.2014.11.016

禽戊型肝炎病毒组织切片免疫荧光检测方法的建立及应用

陈瑞爱^1,2,3*，李延鹏³，同戈¹，郭凯¹，刘正伟¹

（1.华南农业大学兽医学院，广州 510642；2.农业部动物疫病防控生物技术与制品创制重点实验室，肇庆 526238；3.广东省兽用生物制品生物技术研究与应用企业重点实验室，肇庆 526238）

收稿日期:2014-03-31 出版日期:2014-11-23 发布日期:2014-11-24
通讯作者: 陈瑞爱
作者简介:陈瑞爱(1970-)，女，广东新兴人，教授，主要从事免疫抑制病研究，E-mail:chenruiaidhn@126.com
基金资助:
重大动物疫病新型疫苗的关键技术研究与产业化（2011A090200117）

Establishment and Application of Tissue Section Immunofluorescence Method for Detecting Avian Hepatitis E Virus

CHEN Rui-ai^1,2,3*，LI Yan-peng³，TONG Ge¹，GUO Kai¹，LIU Zheng-wei¹

（1.College of Veterinary Medicine，South China Agricultural University，Guangzhou 510642，China；2.Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention of Ministry of Agriculture，P.R.China，Zhaoqing 526238，China；3.Guangdong Provincial Key Laboratory of Biotechnology R & D of Veterinary Biological Products，Zhaoqing 526238，China）

Received:2014-03-31 Online:2014-11-23 Published:2014-11-24
Supported by:

摘要/Abstract

摘要：

为建立对禽戊型肝炎病毒（HEV）鉴别诊断的组织切片免疫荧光方法，制备禽HEV ORF2蛋白的单因子血清，对感染HEV、禽白血病病毒（ALV）、禽网状内皮组织增生症病毒（REV）鸡的病变肝组织切片进行间接免疫荧光检测。结果显示制备的禽HEV ORF2蛋白的单因子血清具有良好的特异性，利用组织切片免疫荧光方法能够特异地检测禽HEV的感染，从而有效地区分HEV与易混淆的ALV、REV的感染。建立的鉴别诊断禽HEV的组织切片免疫荧光方法，可以有效地诊断禽HEV感染，给实验室诊断带来极大的方便。

关键词: 禽戊型肝炎病毒, 组织切片, 免疫荧光, 鉴别诊断

Abstract:

Immunofluorescence staining method on paraffin-embedded liver sections was established to differentially detect avian HEV in this experiment.Mono-specific serum of HEV ORF2 protein were prepared and used for detecting tissue section of lesion liver of chickens infected with HEV，avian leukosis virus(ALV)，reticuloendotheliosis virus (REV)，respectively.Results showed that the prepared mono-specific serum of HEV ORF2 protein showed good specificity.The established immunofluorescence method could detect avian HEV infection and was effective to distinguish HEV from ALV or REV infection.Our results revealed that the novel immunofluorescence method brings great convenience for laboratory diagnosis.

Key words: avian hepatitis E virus, tissue section, immunofluorescence, differential diagnosis

中图分类号:

陈瑞爱，李延鹏，同戈，郭凯，刘正伟. 禽戊型肝炎病毒组织切片免疫荧光检测方法的建立及应用[J]. 畜牧兽医学报, 2014, 45(11): 1851-1857.

CHEN Rui-ai，LI Yan-peng，TONG Ge，GUO Kai，LIU Zheng-wei. Establishment and Application of Tissue Section Immunofluorescence Method for Detecting Avian Hepatitis E Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1851-1857.

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禽戊型肝炎病毒组织切片免疫荧光检测方法的建立及应用

Establishment and Application of Tissue Section Immunofluorescence Method for Detecting Avian Hepatitis E Virus

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