miR-M7-5p靶向调控马立克病病毒原癌基因meq的表达

doi:10.11843/j.issn.0366-6964.2014.09.019

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (9): 1518-1525.doi: 10.11843/j.issn.0366-6964.2014.09.019

miR-M7-5p靶向调控马立克病病毒原癌基因meq的表达

赵朴^1,2,4，滕蔓²，罗俊²，迟佳琦²，宿靖伟³，党露^1,2，邓瑞广²，张改平^2，3*

(1.西北农林科技大学动物医学院，杨凌 712100；2.河南省农业科学院，农业部动物免疫学重点实验室，河南省动物免疫学重点实验室，郑州 450002；3.河南农业大学牧医工程学院，郑州 450002；4.河南科技学院动物科学学院，新乡 453003）

收稿日期:2014-04-10 出版日期:2014-09-23 发布日期:2014-09-23
通讯作者: 张改平，E-mail：zhanggaiping2003@163.com
作者简介:赵朴（1975-），男，河南南阳人，博士生，主要从事动物病毒学与免疫学研究，E-mail:zhpu2008@163.com
基金资助:
国家自然科学基金项目(31372445)；河南省农业科学院优秀青年科技基金（2013YQ28）

Marek’s Disease Virus Oncogene meq is Targeted by the Viral microRNA miR-M7-5p

ZHAO Pu^1,2,4，TENG Man²，LUO Jun²，CHI Jia-qi²，SU Jing-wei³，DANG Lu^1,2，DENG Rui-guang²，ZHANG Gai-ping^2，3*

（1.College of Veterinary Medicine，Northwest A & F University，Yangling 712100，China；2.Key Laboratory of Animal Immunology of the Ministry of Agriculture，Henan Provincial Key Laboratory of Animal Immunology，Henan Academy of Agricultural Sciences，Zhengzhou 450002，China；3.College of Animal Science and Veterinary Medicine，Henan Agricultural University，Zhengzhou 450002，China；4.Department of Animal Science and Technology，Henan Institute of Science and Technology，Xinxiang 453003，China）

Received:2014-04-10 Online:2014-09-23 Published:2014-09-23

摘要/Abstract

摘要：

马立克病病毒（MDV）基因组编码数十个miRNA，并且可能在MDV的感染及致病过程中发挥重要作用。此前研究发现， MDV-1编码的miR-M7-5p在病毒感染宿主发病的过程中具有高水平表达特征，为探讨miR-M7-5p对MDV可能具有的自我调控作用，利用生物信息学方法对MDV编码的蛋白基因进行了扫描分析，预测发现了10个潜在的miR-M7-5p结合靶点，其中包括MDV的原癌基因meq。双荧光报告试验表明miR-M7-5p与meq基因的3′-UTR在体外能够相互作用，qRT-PCR进一步证实miR-M7-5p在体内能够下调meq基因mRNA的转录。结果表明，miR-M7-5p能够自我调控病毒原癌基因meq的表达，在MDV致瘤过程中可能发挥重要作用。

关键词: 马立克病病毒, microRNA, 自我调控, 原癌基因, meq

Abstract:

In the viral genomes of Marek’s disease virus (MDV)，dozens of miRNAs have been identified and some of them are suggested to potentially play important roles in MDV pathogenesis and oncogenesis.Previous studies have shown that the MDV-1-encoded miR-M7-5p is expressed at a high level during different phases of the developing disease.To investigate the potential self-regulation of miR-M7-5p on MDV during virus infection，we have presently scanned the MDV protein-coding genes utilizing bioinformatics method and obtained 10 potential target candidates for miR-M7-5p，including the most important viral oncogene meq.Dual luciferase reporter assay (DLRA) showed that miR-M7-5p can interact with the 3′-UTR of meq gene.Using real-time quantitative RT-PCR，we further confirmed that the transcription level of meq gene in MDV-infected CEF was down regulated by miR-M7-5p.Our data suggests that the oncogene meq is self-regulated by the viral miRNA miR-M7-5p in the infection processes，which may contribute to MDV oncogenesis.

Key words: Marek’s disease virus, microRNA, self-regulation, oncogene, meq

中图分类号:

S852.659.1

赵朴，滕蔓，罗俊，迟佳琦，宿靖伟，党露，邓瑞广，张改平. miR-M7-5p靶向调控马立克病病毒原癌基因meq的表达[J]. 畜牧兽医学报, 2014, 45(9): 1518-1525.

ZHAO Pu，TENG Man，LUO Jun，CHI Jia-qi，SU Jing-wei，DANG Lu，DENG Rui-guang，ZHANG Gai-ping. Marek’s Disease Virus Oncogene meq is Targeted by the Viral microRNA miR-M7-5p[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1518-1525.

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miR-M7-5p靶向调控马立克病病毒原癌基因meq的表达

Marek’s Disease Virus Oncogene meq is Targeted by the Viral microRNA miR-M7-5p

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