高糖诱导山羊骨骼肌卫星细胞成脂分化过程中相关基因表达的变化

doi:10.11843/j.issn.0366-6964.2014.05.005

摘要/Abstract

摘要：

本研究采用高糖培养基诱导山羊骨骼肌卫星细胞（Skeletal muscle satellite cells，SMSCs）成脂分化，检测诱导分化过程中脂肪酸合成及成脂分化相关基因表达量，为进一步揭示SMSCs的成脂分化机理提供依据。采用含25 mmol•L^-1葡萄糖的F12培养基诱导山羊SMSCs成脂分化，油红O染色观察脂肪滴形成，qPCR检测诱导前（0 d）和分别在高糖、低糖培养基中培养2 、4 、6 、8 、10 d时脂肪酸合成及成脂分化相关基因ACC、FAS、C/EBPα、PPARγ、SREBP-1、AdipoQ mRNA的相对表达量。结果表明：油红O染色观察到山羊SMSCs高糖培养6 d即形成较多脂肪滴，而低糖培养至10 d也无明显脂肪滴形成。ACC、C/EBPα表达量在高糖培养2 d后显著升高并维持较高值（P<0.05）；SREBP-1、FAS表达量在高糖培养6 d后显著升高（P<0.05）；PPARγ表达量在高糖培养10 d后显著升高（P<0.05）；AdipoQ表达量在高糖培养2、8、10 d时显著升高（P<0.05）。本试验发现高浓度葡萄糖能直接或间接上调脂肪酸合成及成脂肪分化相关基因的表达，促进山羊SMSCs向类脂肪细胞转化。

关键词: 山羊, 骨骼肌卫星细胞, 成脂分化, mRNA表达

Abstract:

This study aimed to induce skeletal muscle satellite cells (SMSCs) to differentiate into adipocytes by using high concentration glucose，and detect the relative expressions of adipogenic related and adipogenic differentiation related genes，and lay a foundation for futher studying the mechanism of SMSCs adipogenic differentiation.High concentration glucose (25 mmol•L^-1) F12 culture medium was used for inducing，and cells were stained with Oil Red O，qPCR was employed to detect the relative expressions of ACC，FAS，C/EBPα，PPARγ，SREBP-1 and AdipoQ at 0 d and five time points (2，4，6，8 and 10 d) under the condition of high and low concentration glucose.The results showed that lots of lipid droplets were observed in cells induced by high concentration glucose at the 6 d.In high concentration glucose group，the mRNA expressions of ACC and C/EBPα significantly increased at the 2 d (P<0.05) and maintained relative high expression levels at later stages，and the expressions of SREBP-1 and FAS were both significantly up-regulated at the 6 d (P<0.05)，while the expression of PPARγ significantly increased at the 10 d (P<0.05).The expression of AdipoQ significantly ascended at the 2，8 and 10 d (P<0.05).In this study，we found that high concentration glucose up-regulated the expressions of the adipogenic-related genes and promoted the SMSCs differentiating into adipocyte-like cells.

Key words: goat, SMSCs, adipogenic differentiation, mRNA expression

中图分类号:

S827
S813.3

薛科，王林杰，陈利，王薏琳，仲涛，李利，张红平. 高糖诱导山羊骨骼肌卫星细胞成脂分化过程中相关基因表达的变化[J]. 畜牧兽医学报, 2014, 45(5): 706-713.

XUE Ke， WANG Lin-jie, CHEN Li， WANG Yi-lin， ZHONG Tao， LI Li， ZHANG Hong-ping. Adipogenic-related Gene Expressions in Goat Skeletal Muscle Satellite Cells Treated with High Concentration Glucose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 706-713.

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