关键词: 伪狂犬病病毒, Guizhou-DY株, 分离鉴定, 致病性

Abstract:

One pseudorabies virus was isolated from aborted swine fetus of a hoggery of Guizhou province, and was identified. It was named as the PRV Guizhou-DY strain (GenBank accession number JX417716). A lot of experiments had done in the study, e g heat resistance test, acid resistance test and fat-soluble sensitivity test, identification of nucleic acid type, and virus pathogenicity test. The inoculated Vero cells showed the typical cytopathogenic effect when cultured to the second generation, and the stable cytopathogenic effect occurred till the fourth generation. By transmission electron microscopy, the mature virions with envelope were round or ellipse with a diameter of 120 to 180 nm, and viral inclusion bodies arranged like crystal lattice were visible in cell nuclei. The rabbits injected with the isolated virus showed the typical clinical syndrome of pruritus, palsy, opisthotonos and death in the end. Experiments on the virus's physicochemical properties showed that PRV Guizhou-DY Strain was a heat-resistant but not acid and chloroform sensitive DNA virus. Sequence analysis of gE genome showed that PRV Guizhou-DY Strain had some variation compared with GZ-Z1 strain, Fa strain and Ea strain, the homology was 97.6%, 98.9% and 99.4%. And its TCID₅₀ was 10^-9.71·100 μL^-1, PFU was 10⁹·mL^-1. The challenge group pigs developed a fever, and the clinical symptoms are more obvious, neutralizing antibodies began to appear at the 5^th day post infection and at its peak at the 21^st day post infection. This study can provide reference for the study of the viral etiology.

Key words: PRV, Guizhou-DY strain, isolation and identification, pathogenicity