L-精氨酸对IUGR仔猪胰岛结构及功能的调控研究

doi:10.11843/j.issn.0366-6964.2013.11.012

摘要/Abstract

摘要：

本试验旨在研究L-精氨酸（L-Arg）对子宫内发育迟缓（IUGR）仔猪胰腺生长发育的调控。试验选取12头IUGR仔猪和6头正常仔猪，哺乳至7 d时断奶，将IUGR仔猪随机分为2组，分别饲喂基础人工乳（IUGR组）、基础人工乳+0.6% Arg（IUGR+Arg组），正常仔猪饲喂基础人工乳（NBW组）。饲喂至14 d时每组选取4头屠宰取样，并对胰腺发育相关指标进行测定。结果表明：受IUGR影响，仔猪胰腺组织中胰岛素（Ins）含量、胰岛面积及β细胞质量均显著降低（P<0.05），胰岛细胞数目、Ins染色阳性率、Ins阳性表达面积及Ins阳性表达细胞数目均极显著减少（P<0.01）。IUGR猪补充Arg后，胰腺绝对重量显著增加（P<0.05）；Ins含量、胰岛面积、胰岛细胞数目、β细胞质量、Ins染色阳性率、Ins阳性表达面积也明显增加（P<0.01），且均与NBW猪相比差异不显著（P>0.05）；胰岛平均染色光密度明显增加（P<0.01），且显著高于NBW猪（P<0.01）。结果提示，IUGR损害仔猪胰腺发育，补充Arg后，IUGR仔猪胰岛结构得到改善，胰岛β细胞数量增加，胰岛素合成增多。

关键词: 宫内发育迟缓, 仔猪, L-精氨酸, 胰岛结构, 胰岛素合成

Abstract:

This study was conducted to study the regulation effects of L-Arg on the growth and development of pancreas in intrauterine growth retardation (IUGR) piglets. Twelve IUGR piglets and six normal birth weight (NBW) piglets (Duroc×Landrace×Yorkshire) were selected. All piglets were weaned at 7 days of age. Then the IUGR piglets were divided into two groups: IUGR group and IUGR+Arg group, which were supplied with based artificial milk and based artificial milk supplementation with 0.6% Arg, respectively. NBW group was supplied with based artificial milk. Four piglets in each group were slaughtered and sampled at 14 days of age, and the indexes about development of pancreas were measured. The results showed as follows: Pancreatic insulin content, islet area and β-cell mass of IUGR piglets were lower than those of NBW piglets (P<0.05). IUGR significantly reduced the number of islet cells, insulin-positive cell fraction, insulin-positive area and the number of insulin-positive cells (P<0.01). Compared with IUGR control piglets, supplementation with Arg increased body weight and absolute pancreas weight of IUGR piglets (P<0.05), and enhanced pancreatic insulin content, islet area and number of islet cells, as well as β-cell mass, insulin-positive cell fraction and insulin-positive area(P<0.01), which were no significant differences with NBW piglets(P>0.05).The average optical density of IUGR piglets increased by Arg supplementation (P<0.01) and were much higher than that of NBW piglets (P<0.01). These results indicated that IUGR impaired the development of pancreas. L-Arg improved the pancreatic islet structure of IUGR piglets, and increased β-cell mass and insulin synthesis.

Key words: IUGR, piglet, L-arginine, pancreatic islet structure, insulin synthesis

中图分类号:

S828
S815.4

孔令蕊，王远孝，李伟，董丽，张婧菲，王恬. L-精氨酸对IUGR仔猪胰岛结构及功能的调控研究[J]. 畜牧兽医学报, 2013, 44(11): 1781-1789.

KONG Ling-rui, WANG Yuan-xiao, LI Wei, DONG Li, ZHANG Jing-fei, WANG Tian. Effects of L-Arg Supplementation on Pancreatic Islet Structure and Function in Intrauterine Growth Retardation Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1781-1789.

参考文献

［1］WU G, BAZER F W, WALLACE J M, et al. Board-invited review: intrauterine growth retardation: implications for the animal sciences［J］. J Anim Sci, 2006, 84(9):2316-2337.

［2］WU G, BAZER F W, CUDD T A, et al. Maternal nutrition and fetal development［J］. J Nutr, 2004, 134(9):2169-2172.

［3］WANG T, HUO Y J, SHI F, et al. Effects of intrauterine growth retardation on development of the gastrointestinal tract in neonatal pigs［J］. Biol Neonate, 2005, 88(1):66-72.

［4］石现瑞. 子宫内生长阻滞对新生仔猪胰腺及胃发育的影响［D］．南京：南京农业大学，2003.

［5］HARADA E, SHIZUYAMA M, IHARA N, et al. Impaired pancreatic endocrine and exocrine responses in growth-retarded piglets［J］. J Vet Med, 2003, 50(9):433-441.

［6］STYRUD J, ERIKSSON U J, GRILL V, et al. Experimental intrauterine growth retardation in the rat causes a reduction of pancreatic B-cell mass, which persists into adulthood［J］. Biol Neonate, 2005, 88(2):122-128.

［7］INOUE T, KIDO Y, ASAHARA S, et al. Effect of intrauterine undernutrition during late gestation on pancreatic beta cell mass［J］. Biomed Res, 2009, 30(6):325-330.

［8］DUMORTIER O, BLONDEAU B, DUVILLIE B, et al. Different mechanisms operating during different critical time-windows reduce rat fetal beta cell mass due to a maternal low-protein or low-energy diet［J］. Diabetologia, 2007, 50(12):2495-2503.

［9］LIMESAND S W, JENSEN J, HUTTON J C, et al. Diminished beta-cell replication contributes to reduced beta-cell mass in fetal sheep with intrauterine growth restriction［J］. Am J Physiol Regul Integr Comp Physiol, 2005, 288(5):R1297-1305.

［10］LIMESAND S W, ROZANCE P J, ZERBE G O, et al. Attenuated insulin release and storage in fetal sheep pancreatic islets with intrauterine growth restriction［J］. Endocrinology, 2006, 147(3):1488-1497.

［11］YUAN Q X, ZHOU J Y, TENG L P, et al. Intrauterine growth retardation leads to the functional change of insulin secretion in the newborn rats［J］. Horm Metab Res, 2010, 42(7):491-495.

［12］REUSENS B, REMACLE C. Programming of the endocrine pancreas by the early nutritional environment［J］. Int J Biochem Cell Biol, 2006, 38(5-6):913-922.

［13］SCHWITZGEBEL V M, SOMM E, KLEE P. Modeling intrauterine growth retardation in rodents: Impact on pancreas development and glucose homeostasis［J］. Mol Cell Endocrinol, 2009, 304(1-2):78-83.

［14］陈才勇, 周根来, 王恬. 以猪为动物模型对子宫内生长阻滞的研究进展［J］. 实验动物科学与管理, 2003, (1):33-36, 45.

［15］WU G, BAZER F W, DAVIS T A, et al. Arginine metabolism and nutrition in growth, health and disease［J］. Amino Acids, 2009, 37(1):153-168.

［16］KIM S W, WU G. Dietary arginine supplementation enhances the growth of milk-fed young pigs［J］. J Nutr, 2004, 134(3):625-630.

［17］COCHARD A, GUILHERMET R, BONNEAU M. Effects of arginine, growth hormone-releasing hormone (GHRH) and neostigmine administered singly or in paired combinations on growth hormone (GH) release in pigs［J］. Reprod Nutr Dev, 1997, 37(5):589-598.

［18］MATEO R D, WU G, BAZER F W, et al. Dietary L-arginine supplementation enhances the reproductive performance of gilts［J］. J Nutr, 2007, 137(3):652-656.

［19］MATEO R D, WU G, MOON H K, et al. Effects of dietary arginine supplementation during gestation and lactation on the performance of lactating primiparous sows and nursing piglets［J］. J Anim Sci, 2008, 86(4):827-835.

［20］GIOVANNUCCI E. Insulin, insulin-like growth factors and colon cancer: a review of the evidence［J］. J Nutr, 2001, 131(11 Suppl):3109S-3120S.

［21］姚康, 褚武英, 邓敦, 等. 不同精氨酸添加水平对哺乳仔猪生长性能的影响［J］. 天然产物研究与开发, 2008, (1):121-124.

［22］BURKE C, SINCLAIR K, COWIN G, et al. Intrauterine growth restriction due to uteroplacental vascular insufficiency leads to increased hypoxia-induced cerebral apoptosis in newborn piglets［J］. Brain Res, 2006, 1098(1):19-25.

［23］YAO K, YIN Y L, CHU W, et al. Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs［J］. J Nutr, 2008, 138(5):867-872.

［24］孔祥峰, 尹富贵, 刘合军, 等. 早期断奶仔猪生理生化参数和脏器指数的变化［J］. 中国实验动物学报, 2006, (4):298-302.

［25］YAMAMOTO M, KATAOKA K. An electron microscope study of the development of the exocrine and endocrine pancreas with special reference to intercellular junctions［J］. Arch Histol Cytol, 1988, 51(4):315-325.

［26］REHFELDT C, KUHN G. Consequences of birth weight for postnatal growth performance and carcass quality in pigs as related to myogenesis［J］. J Anim Sci, 2006, 84 (Suppl): E113-123.

［27］XU R J, MELLOR D J, BIRTLES M J, et al. Impact of intrauterine growth retardation on the gastrointestinal tract and the pancreas in newborn pigs［J］. J Pediatr Gastroenterol Nutr, 1994, 18(2):231-240.

［28］WOLTER B F, ELLIS M, CORRIGAN B P, et al. The effect of birth weight and feeding of supplemental milk replacer to piglets during lactation on preweaning and postweaning growth performance and carcass characteristics［J］. J Anim Sci, 2002, 80(2):301-308.

［29］FERNANDEZ-TWINN D S, OZANNE S E. Mechanisms by which poor early growth programs type-2 diabetes, obesity and the metabolic syndrome［J］. Physiol Behav, 2006, 88(3):234-243.

［30］LUCAS A. Programming by early nutrition: an experimental approach［J］. J Nutr, 1998, 128(2Suppl): 401S-406S.

［31］VAN SCHRAVENDIJK C F, FORIERS A, HOOGHE-PETERS E L, et al. Pancreatic hormone receptors on islet cells［J］. Endocrinology, 1985, 117(3):841-848.

［32］HARBECK M C, LOUIE D C, HOWLAND J, et al. Expression of insulin receptor mRNA and insulin receptor substrate 1 in pancreatic islet beta-cells［J］. Diabetes, 1996, 45(6):711-717.

［33］BERTIN E, GANGNERAU M N, BAILBE D, et al. Glucose metabolism and beta-cell mass in adult offspring of rats protein and/or energy restricted during the last week of pregnancy［J］. Am J Physiol, 1999, 277(1 Pt 1):E11-17.

［34］LEIBIGER B, WAHLANDER K, BERGGREN P O, et al. Glucose-stimulated insulin biosynthesis depends on insulin-stimulated insulin gene transcription［J］. J Biol Chem, 2000, 275(39):30153-30156.

［35］ASPINWALL C A, LAKEY J R, KENNEDY R T. Insulin-stimulated insulin secretion in single pancreatic beta cells［J］. J Biol Chem, 1999, 274(10):6360-6365.

［36］ASPINWALL C A, QIAN W J, ROPER M G, et al. Roles of insulin receptor substrate-1, phosphatidylinositol 3-kinase, and release of intracellular Ca²⁺ stores in insulin-stimulated insulin secretion in beta -cells［J］. J Biol Chem, 2000, 275(29):22331-22338.

［37］王远孝, 张莉莉, 周根来, 等. 精氨酸对IUGR仔猪器官指数和血清激素水平的影响［C］//低碳经济与高效养殖——第六次全国饲料营养学术研讨会暨动物营养学分会成立三十周年大会，2010:1.

［38］GRADWOHL G. Development of the endocrine pancreas［J］. Diabetes Metab, 2006, 32(5 Pt 2):532-533.

［39］胡劲涛. 氧化损伤在低出生体重仔鼠发生代谢综合征过程中的作用及干预研究［D］．长沙：中南大学，2008.

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