4株猪肺炎支原体脂膜蛋白对猪肺泡上皮细胞生长抑制作用的比较

doi:10.11843/j.issn.0366-6964.2013.08.016

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (8): 1283-1287.doi: 10.11843/j.issn.0366-6964.2013.08.016

4株猪肺炎支原体脂膜蛋白对猪肺泡上皮细胞生长抑制作用的比较

倪博^1,2，白方方²，韦艳娜²，刘茂军²，冯志新²，熊祺琰²，魏建忠¹，邵国青^2*

(1. 安徽农业大学动物科技学院，合肥 230036；2. 江苏省农业科学院兽医研究所·农业部兽用生物制品工程技术重点实验室·国家兽用生物制品工程技术研究中心，南京 210014)

收稿日期:2013-03-04 出版日期:2013-08-23 发布日期:2013-08-27
通讯作者: 邵国青，男，博士，研究员，Tel：025-84391973，E-mail:84391973@163.com
作者简介:倪博（1988-），女，在读硕士研究生，主要从事动物疫病防控研究，E-mail: nanjingnibo@163.com
基金资助:
国家自然科学基金（31100135）；江苏省农业自主创新基金（CX（11）4038）

Comparative Analysis of the Inhibitory Effects of Lipid Associated Membrane Proteins from Four Different Strains of Mycoplasma hyopneumoniae on Porcine Alveolar Epithelial Cell Lines

NI Bo^1,2, BAI Fang-fang², WEI Yan-na², LIU Mao-jun², FENG Zhi-xin²,XIONG Qi-yan², WEI Jian-zhong¹, SHAO Guo-qing^2*

(1. College of Animal Science, Anhui Agricultural University, Hefei 230036, China; 2. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of VeterinaryBiological Engineering and Technology of Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China)

Received:2013-03-04 Online:2013-08-23 Published:2013-08-27

摘要/Abstract

摘要：

比较不同毒力猪肺炎支原体的脂膜蛋白（LAMPs）对猪肺泡上皮细胞生长抑制作用的差异。使用Trion-X114从猪肺炎支原体培养物中提取LAMPs，采用MTT法检测LAMPs对细胞生长的抑制率，观察其对细胞生长状况的影响，并使用Griess试剂对细胞培养物中NO的浓度变化进行测定。结果表明，4株猪肺炎支原体的LAMPs均对猪肺泡上皮细胞SJPL有抑制作用，其抑制作用的强度与猪肺炎支原体的毒力强弱基本相符，即NJ株与168株的抑制作用较强，而168致弱株及J株对SJPL细胞的抑制作用较弱。其中，NJ株的IC₅₀最低，为0.13 mg·mL^－1；而J株的IC₅₀最高，为0.56 mg·mL^－1，并且LAMPs可以诱导细胞产生NO。猪肺炎支原体LAMPs对猪肺泡上皮细胞的抑制作用与支原体的毒力相关，强毒株LAMPs的抑制作用较大，而弱毒株的抑制作用较小。

关键词: 猪肺炎支原体, LAMPs, 细胞抑制, NO

Abstract:

The objective of this study was to investigate the inhibition of lipid associated membrane proteins (LAMPs) from Mycoplasma hyopneumoniae of different virulence on porcine alveolar epithelial cell lines. LAMPs were extracted from the culture of M. hyopneumoniae by Trion-X114, the suppression ratio was detected through MTT assay, and moreover the concentration of nitric oxide was measured by Griess reagent. Our results indicate that all of four kinds LAMPs can do toxic action on porcine alveolar epithelial cell lines, and the containment procedure was agreed with the virulence of M. hyopneumoniae. The inhibited effect caused by strain NJ and 168 was stronger than strain attenuate 168 and J, which the minimum IC₅₀ (0.13 mg·mL^－1) belonged to strain NJ and the maximum IC₅₀ (0.56 mg·mL^－1) belonged to strain J. Moreover, LAMPs also could induce NO. The inhibition on porcine alveolar epithelial cell lines induced by M. hyopneumoniae LAMPs is related with the virulence of Mycoplasma, while the inhibited effect of virulent strain is more powerful than attenuate strain.

Key words: M. hyopneumoniae, LAMPs, cyto-inhibition, NO

中图分类号:

S852.62

倪博，白方方，韦艳娜，刘茂军，冯志新，熊祺琰，魏建忠，邵国青. 4株猪肺炎支原体脂膜蛋白对猪肺泡上皮细胞生长抑制作用的比较[J]. 畜牧兽医学报, 2013, 44(8): 1283-1287.

NI Bo, BAI Fang-fang, WEI Yan-na, LIU Mao-jun, FENG Zhi-xin,XIONG Qi-yan, WEI Jian-zhong, SHAO Guo-qing. Comparative Analysis of the Inhibitory Effects of Lipid Associated Membrane Proteins from Four Different Strains of Mycoplasma hyopneumoniae on Porcine Alveolar Epithelial Cell Lines[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1283-1287.

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4株猪肺炎支原体脂膜蛋白对猪肺泡上皮细胞生长抑制作用的比较

Comparative Analysis of the Inhibitory Effects of Lipid Associated Membrane Proteins from Four Different Strains of Mycoplasma hyopneumoniae on Porcine Alveolar Epithelial Cell Lines

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