UCP2基因遗传多态性与西门塔尔牛生长相关性状的遗传效应分析

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (5): 708-716.

UCP2基因遗传多态性与西门塔尔牛生长相关性状的遗传效应分析

陈翠，张立敏，陈晓杰，刘喜冬，张猛，李姣，袁峥嵘，张路培，高雪，高会江,李俊雅，许尚忠*

中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室，北京 100193

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 许尚忠

Associations of UCP2 Gene Polymorphisms with Growth Traits in Simmental

CHEN Cui, ZHANG Limin, CHEN Xiaojie, LIU Xidong, ZHANG Meng, LI Jiao, YUAN Zhengrong, ZHANG Lupei, GAO Xue,GAO Huijiang, LI Junya, XU Shangzhong*

Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
Contact: XU Shangzhong*

摘要/Abstract

摘要： 本研究旨在分析UCP2基因SNPs及其单倍型与肉牛饲料转化率和体尺等生长相关性状的相关性。以118头西门塔尔牛为试验材料，利用DNA池和PCRRFLP方法检测UCP2基因G118A、 C161T、 C215G、C305T位点多态性，构建单倍型并与生长性状进行相关分析。结果表明，G118A、C161T和C305T位点均与饲料转化率显著相关(P<0.05)，其中杂合个体均值显著高于纯合个体且料肉比较高,而纯合个体之间差异不显著。位点C161T和C305T对胸围和腹围发挥主要影响作用，在差异显著表型性状中，TT基因型显著大于CC基因型个体。所构建的单倍型与单个SNP分析结果较为一致。综合考虑饲料转化率、平均日增体质量、胸围和腹围，建议将C161T和C305T位点的纯合个体TT基因型作为提高肉牛生长性状的候选分子标记。

关键词: UCP2, 生长性状, 单倍型, 关联分析

Abstract: The aim of this study was to analyze the association of UCP2 gene polymorphisms and haplotypes with feed conversion ratio(FCR) and growth traits in beef cattle. In this study, 118 Simmental were used, four SNPs of UCP2 gene (G118A，C161T，C215G，C305T) were genetyped by PCRRFLP in Simmental to investigate the associations of SNPs and haplotypes with FCR and growth traits. The results showed that G118A, C161T and C305T were significantly associated with FCR (P<0.05).Compared with the individuals with homozygote, the values of FCR of individuals with the heterozygote were significantly higher. However, the difference between the individuals with homozygotes were not significant. The locus C161T and C305T had an major affect on heart girth and circumference of abdomen. Among the phenotypes which were significantly different, individuals with TT genotype had a significantly higher average value than that of individuals with AA genotype. Association analysis of haplotypes indicated similar results with that of SNPs. Considered the FCR,ADG and body size traits, it was suggested that the TT genotype at locus C161T and C305T of UCP2 gene were important candidate molecular markers for improving growth traits.

Key words: UCP2, growth trait, haplotype, association analysis

陈翠;张立敏;陈晓杰;刘喜冬;张猛;李姣;袁峥嵘;张路培;高雪;高会江;李俊雅;许尚忠. UCP2基因遗传多态性与西门塔尔牛生长相关性状的遗传效应分析[J]. 畜牧兽医学报, 2012, 43(5): 708-716.

CHEN Cui;ZHANG Limin;CHEN Xiaojie;LIU Xidong;ZHANG Meng;LI Jiao;YUAN Zhengrong;ZHANG Lupei;GAO Xue;GAO Huijiang;LI Junya;XU Shangzhong. Associations of UCP2 Gene Polymorphisms with Growth Traits in Simmental[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(5): 708-716.

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