视黄醇对LPS诱发的原代培养大鼠乳腺上皮细胞炎症反应的调节

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (4): 627-633.

视黄醇对LPS诱发的原代培养大鼠乳腺上皮细胞炎症反应的调节

顾蓓蓓1,2，苗晋锋1，卢劲晔1，邹思湘1*

1. 南京农业大学动物医学院，南京 210095； 2. 泰州出入境检验检疫局，泰州 225300

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-04-25 发布日期:2012-04-25
通讯作者: 邹思湘

Modulation of Retinoic Acid in LPSinduced Inflammation in Primary Culture of Rat Mammary Epithelial Cells

GU Beibei1, 2, MIAO Jinfeng1, LU Jinye1, ZOU Sixiang1*

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; 2. Taizhou EntryExit Inspection and Quarantine Bureau of the P.R.C, Taizhou 225300, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-04-25 Published:2012-04-25
Contact: ZOU Sixiang

摘要/Abstract

摘要： 本研究旨在优化大鼠乳腺上皮细胞培养体系，建立LPS诱发的炎症反应模型，研究视黄醇对其炎症反应的调节机制。采用胶原酶与透明质酸酶联合消化分离大鼠乳腺上皮细胞。待细胞铺满整个培养瓶的90%时，（1）用不同浓度的 LPS处理乳腺上皮细胞，24 h收集细胞及培养上清液；（2）分为试验组（添加1 μmol·L-1 视黄醇）和对照组，处理24 h后更换培养液，用10 μg·mL-1的LPS处理细胞，分别于不同时间点收集细胞。结果表明：LPS处理大鼠乳腺上皮细胞后引起各个时间点炎性因子mRNA表达极显著升高，视黄醇能显著下调上述炎性细胞因子的表达。视黄醇预处理能减轻LPS诱发的大鼠乳腺上皮细胞的炎症损伤。

关键词: 脂多糖, 乳腺上皮细胞, 视黄醇, 炎性反应

Abstract: Lipopolysaccharide (LPS) induced primary culture of rat mammary epithelial cells (rMEC) inflammatory model was established and the protective mechanism of retinoic acid was studied. rMEC s were separated through digestion by collagenase and hyaluronidase. When cells were grown to 90% confluence in 6well plates, (1) cells were treated with different concentrations of LPS; (2) cells were treated with 1 μmol·L-1 retinoic acid before stimulation with 10 μg· mL-1 LPS. Cells and supernatants were collected. In this study, inflammatory models of primary culture of rMEC were successfully established. Stimulation of rMEC with LPS elicited a marked increase in mRNA expression for inflammatory mediators. Treatment of retinoic acid significantly decreased the mRNA expression of those mediators. Retinoic acid could reduce LPSinduced inflammatory reaction. These results further indicate that retinoic acid may protect mastitis from proinflammatory cytokinemediated damage of mammary epithelial cells.

Key words: lipopolysaccharide, mammary epithelial cells, retinoic acid, inflammatory model

顾蓓蓓;苗晋锋;卢劲晔;邹思湘. 视黄醇对LPS诱发的原代培养大鼠乳腺上皮细胞炎症反应的调节[J]. 畜牧兽医学报, 2012, 43(4): 627-633.

GU Beibei;;MIAO Jinfeng;LU Jinye;ZOU Sixiang . Modulation of Retinoic Acid in LPSinduced Inflammation in Primary Culture of Rat Mammary Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(4): 627-633.

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