中国美利奴羊DLX3基因3′UTR的多态性及其与羊毛品质性状的关联分析

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (3): 358-367.

中国美利奴羊DLX3基因3′UTR的多态性及其与羊毛品质性状的关联分析

荣恩光1，王志鹏1，张志威1，杨华2，李辉1*，王宁1*

1.东北农业大学动物科学技术学院，哈尔滨150030； 2.新疆农垦科学院畜牧兽医研究所，石河子832000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-03-28 发布日期:2012-03-28
通讯作者: 李辉1*，王宁1

Polymorphism in 3′UTR of DLX3 Gene and Its Association with Wool Quality Traits in Chinese Merino Sheep

RONG Enguang1，WANG Zhipeng1，ZHANG Zhiwei1，YANG Hua2，LI Hui1*，WANG Ning1*

1. College of Animal Science and Technology，Northeast Agricultural University，Harbin 150030，China； 2. Institute of Animal Husbandry and Veterinary，Xinjiang Academy of Agricultural and Reclamation Science， Shihezi 832000，China

Received:1900-01-01 Revised:1900-01-01 Online:2012-03-28 Published:2012-03-28
Contact: LI Hui1*，WANG Ning1*

摘要/Abstract

摘要： 为探讨DLX3基因的多态性与绵羊羊毛品质及生长性状的关系，本研究以5个品系的中国美利奴羊（新疆军垦型）为试验材料，采用PCRRFLP方法开展了DLX3基因3′UTR区4个SNPs的多态性检测。通过卡方检验分析了4个SNPs在各品系绵羊中的等位基因频率，采用连锁不平衡分析构建了这4个SNPs的单倍型，最后将单位点和单倍型分别与绵羊羊毛品质和生长性状进行关联分析。结果表明：4个SNPs在5个品系间的等位基因频率均存在极显著差异（P<0.01）；超细毛绵羊品系与其他4个品系间的基因型分布均存在极显著差异（P<0.01），2个多胎品系与其他3个品系间的基因型分布同样存在极显著差异（P<0.01）；相关分析显示，这4个SNPs及其单倍型均对羊毛卷曲度有显著影响（P<0.05），而对其他羊毛性状及生长性状无显著影响（P>0.05）。由此可见，中国美利奴羊（新疆军垦型）DLX3基因3′UTR区的多态性与绵羊毛发卷曲度性状显著相关，可以尝试使用这些SNPs开展高品质细毛羊的分子标记辅助选择。

关键词: 中国美利奴羊（新疆军垦型）, DLX3基因, SNPs, 3′UTR

Abstract: This experiment was designed to study the effects of polymorphism of DLX3 gene on wool quality and growth traits in sheep. Five strains of Chinese Merino (Xinjiang Junken Type) sheep were used in the present study. The polymorphism in 3′ untranslated region (UTR) of DLX3 gene was detected and identified using DNA sequencing, and SNP genotyping was performed by PCRRFLP analysis. Allele frequencies were analyzed by Chisquare test. Haplotypes were constructed by linkage disequilibrium analysis. The association between the SNPs and haplotypes and wool quality and growth traits were assessed by JMP4.0 software, respectively. The result showed that the allele frequencies for these four SNPs were significantly different among the five Chinese sheep strains (P<0.01). The genotype distribution in super fine wool strain were different from that in the other four strains, and the genotype distribution in two prolificacy strains were significantly different from that in other three noprolificacy strains (P<0.01); Association analysis displayed that the four SNPs and their haplotypes had consistently significant effect on wool curvature (P<0.05), but no significant effect on other traits (P>0.05). The result indicate that polymorphisms of 3′UTR of DLX3 gene are significantly correlated with wool curvature in Chinese Merino (Xinjiang Junken Type) sheep, and these SNPs can be used in molecular MAS for wool quality traits.

Key words: Chinese Merino (Xinjiang Junken Type) sheep, DLX3 gene, SNPs, 3′UTRDLX3（Distalless homeobox 3）

荣恩光;王志鹏;张志威;杨华;李辉;王宁. 中国美利奴羊DLX3基因3′UTR的多态性及其与羊毛品质性状的关联分析[J]. 畜牧兽医学报, 2012, 43(3): 358-367.

RONG Enguang;WANG Zhipeng;ZHANG Zhiwei;YANG Hua;LI Hui;WANG Ning . Polymorphism in 3′UTR of DLX3 Gene and Its Association with Wool Quality Traits in Chinese Merino Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(3): 358-367.

[1]	侯浩宾, 李海静, 杨莉, 张新浩, 石田培, 王欣悦, 赵志达, 张莉. 德州驴NCAPG-DCAF16基因区域多态性与生长性状的关联分析[J]. 畜牧兽医学报, 2019, 50(2): 302-313.
[2]	侯浩宾, 李海静, 杨莉, 张新浩, 石田培, 王欣悦, 赵志达, 张莉. 德州驴NCAPG-DCAF16基因区域多态性与生长性状的关联分析[J]. 畜牧兽医学报, 2019, 50(2): 302-313.
[3]	李英杰，刘铮铸，巩元芳，徐桂利，张磊，唐家明，段玲欣，刘谢荣，郭秀玲，陆奇玉. 狐狸Agouti基因SNPs筛查及其与毛色关联性分析[J]. 畜牧兽医学报, 2015, 46(9): 1692-1696.
[4]	李英杰，刘铮铸，巩元芳，徐桂利，张磊，唐家明，段玲欣，刘谢荣，郭秀玲，陆奇玉. 狐狸Agouti基因SNPs筛查及其与毛色关联性分析[J]. 畜牧兽医学报, 2015, 46(9): 1692-1696.
[5]	王文浩，张涛，王金玉，张跟喜，樊庆灿，陈学森，韩鲲鹏，王永娟. 京海黄鸡新城疫和传染性支气管炎抗病性状的全基因组关联分析[J]. 畜牧兽医学报, 2015, 46(2): 196-203.
[6]	王文浩，张涛，王金玉，张跟喜，樊庆灿，陈学森，韩鲲鹏，王永娟. 京海黄鸡新城疫和传染性支气管炎抗病性状的全基因组关联分析[J]. 畜牧兽医学报, 2015, 46(2): 196-203.
[7]	唐莹,王金玉,张跟喜,樊庆灿,陈学森,张涛,魏岳,施会强. Myf5基因8个SNPs位点与京海黄鸡生长和繁殖性状的关联分析[J]. 畜牧兽医学报, 2014, 45(6): 863-870.
[8]	唐莹,王金玉,张跟喜,樊庆灿,陈学森,张涛,魏岳,施会强. Myf5基因8个SNPs位点与京海黄鸡生长和繁殖性状的关联分析[J]. 畜牧兽医学报, 2014, 45(6): 863-870.
[9]	高建斌，昝林森，杨宁，李耀坤，皇甫一凡，郝瑞杰，马向辉，付常振，姜碧杰，成功. 秦川牛DKK1基因SNPs检测及其与体尺、肉质性状的关联分析[J]. 畜牧兽医学报, 2013, 44(3): 376-386.
[10]	高建斌，昝林森，杨宁，李耀坤，皇甫一凡，郝瑞杰，马向辉，付常振，姜碧杰，成功. 秦川牛DKK1基因SNPs检测及其与体尺、肉质性状的关联分析[J]. 畜牧兽医学报, 2013, 44(3): 376-386.
[11]	李俊营，詹凯，刘伟，韩涛，李绍全，柏如东. 淮南麻黄鸡CRBP4基因多态性与早期产蛋性状和蛋品质的相关性[J]. 畜牧兽医学报, 2013, 44(2): 197-203.
[12]	李俊营，詹凯，刘伟，韩涛，李绍全，柏如东. 淮南麻黄鸡CRBP4基因多态性与早期产蛋性状和蛋品质的相关性[J]. 畜牧兽医学报, 2013, 44(2): 197-203.
[13]	曹顶国;周艳;雷秋霞;韩海霞;李福伟;李桂明;盛倩;王存芳;逯岩;. 鸡VLDLR基因多态性与蛋黄性状的关联分析[J]. 畜牧兽医学报, 2012, 43(6): 849-856.
[14]	曹顶国;周艳;雷秋霞;韩海霞;李福伟;李桂明;盛倩;王存芳;逯岩;. 鸡VLDLR基因多态性与蛋黄性状的关联分析[J]. 畜牧兽医学报, 2012, 43(6): 849-856.
[15]	李晓宁;王慧;于希江;姜运良;宋倩;闫超;唐辉. 文昌鸡MTNR1B基因的多态性及其与产蛋性状的关系[J]. 畜牧兽医学报, 2012, 43(4): 540-545.