畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (12): 1800-1804.

• 研究简报 • 上一篇    

番鸭呼肠孤病毒病活疫苗诱导的免疫应答分析

林锋强1,2，陈仕龙1,2，陈少莺1,2*，朱小丽1,2，程晓霞1,2，胡奇林1,2，王劭1,2   

  1. 1.福建省农业科学院畜牧兽医研究所，福州 350013；2.福建省畜禽疫病防治工程技术研究中心, 福州 350013
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 陈少莺

The Study on Immunne Response of Muscovy Duck Reovirusis Alive Vaccine

LIN Fengqiang1,2，CHEN Shilong1,2，CHEN Shaoying1,2*，ZHU Xiaoli1,2，CHENG Xiaoxia1,2，HU Qilin1,2，WANG Shao1,2   

  1. LIN Fengqiang1,2，CHEN Shilong1,2，CHEN Shaoying1,2*，ZHU Xiaoli1,2，CHENG Xiaoxia1,2，HU Qilin1,2，WANG Shao1,2
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-12-15 Published:2011-12-15
  • Contact: CHEN Shaoying

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摘要： 本研究旨在评价番鸭呼肠孤病毒病活疫苗对番鸭免疫功能的影响，探讨番鸭呼肠孤病毒病活疫苗的免疫机理；1日龄番鸭免疫番鸭呼肠孤病毒病活疫苗，分别于免疫后7~35 d观察番鸭生长发育，检测免疫番鸭血清中和抗体效价，外周血淋巴细胞、胸腺细胞和法氏囊细胞对ConA、LPS的反应，细胞毒T细胞(CTL)的细胞毒性作用。结果显示：免疫番鸭生长发育正常，免疫器官中胸腺明显增大；免疫后35 d血清中和抗体才开始上升；外周血和胸腺淋巴细胞对ConA的增殖反应显著提高（P<0.05）；而外周血和法氏囊淋巴细胞对LPS增殖反应无显著变化（P<0.05）；外周血细胞毒性T细胞杀伤功能增强（P<0.05）。结果提示番鸭呼肠孤病毒病活疫苗免疫后主要刺激提高番鸭细胞免疫功能，提高机体抵抗番鸭呼肠孤病毒的能力。

关键词: 番鸭呼肠孤病毒病活疫苗, 番鸭, 免疫应答, 细胞免疫

Abstract: The experiment was conducted to access the effect of muscovy duck reovirusis alive vaccine on immune response and to explore the immune mechanism. After 1dayold muscovy ducks were immunized, the growth and development were observed, neutralization antibody in the sera, proliferation responses of the lymphocytes in the peripheral blood, thymus and bursa cells to ConA and LPS, Cytotoxic activity of cytotoxic T lymphocytes (CTL) were detected. The results showed that the growth and development of the immunized muscovy ducks were more than that of control group. The neutralization antibody began to increase until 35 days. Lymphocyte proliferation response to ConA in blood and thymus was enhanced differently in the immunized muscovy ducks, but response to LPS in blood and bursa weren’t changed differently. The cytotoxic activity of CTL in the immunized muscovy ducks was improved. So the muscovy duck reovirusis alive vaccine can improve the resistant ability of muscovy ducks to muscovy duck reovirus mainly by increasing cellular immunity．It laid theoretical basis on clinical application with muscovy duck alive vaccine.

Key words: muscovy duck reovirusis, alive vaccine, immunological response, cellular immunity

ISSN 0366-6964
CN 11-1985/S
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