绵羊CS-1基因的克隆及在凉山半细毛羊不同组织中的表达

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (11): 1532-1536.

绵羊CS-1基因的克隆及在凉山半细毛羊不同组织中的表达

苏强强，吴登俊*

四川农业大学动物科技学院动物遗传育种研究所，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25
通讯作者: 吴登俊

Cloning of CS-1 in Ovine and Tissue Expression in Liangshan Semifine Sheep

SU Qiangqiang, WU Dengjun*

Research Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
Contact: WU Dengjun

摘要/Abstract

摘要：

旨在研究绵羊CS1基因序列及其蛋白质的结构与功能以及其在不同组织中的表达情况,进而预测其与肉质性状的关联性。Calsarcins(Calcineurinassociated sarcomeric protein,CS)家族是一个与钙调神经磷酸酶（Calcineurin,CaN）相结合的肌纤维特异表达蛋白新成员,CS1基因是与畜禽肉质性状密切相关的重要候选基因。本研究根据牛、人和鼠CS1基因mRNA，应用比较基因组学技术成功克隆了绵羊CS1基因全长cDNA，对序列及编码的氨基酸进行生物信息学分析。结果显示，该基因cDNA全长951 bp，完整的开放阅读框（ORF）为79～872 bp，编码264个氨基酸。氨基酸序列中存在3个保守结构域，蛋白质二级结构以无规卷曲和α螺旋为主，富含疏水区，存在多个磷酸化位点和1个蛋白激酶C（Protein kinase C,PKC）的磷酸化位点。通过实时荧光定量RTPCR技术分析CS1基因在凉山半细毛羊部分组织中的表达情况。结果表明：CS1基因主要在凉山半细毛羊的心脏和背肌中表达，15 d在心脏的表达量最高，极显著高于其它各组织(P<0.01)；在心脏和背肌中，CS1基因的表达量随着年龄的增长而减少，其中在60 d时心脏中的表达量最高。研究结果为绵羊的肉质性状改善提供科学依据。

关键词: CS1基因, cDNA克隆, 组织表达, 绵羊

Abstract:

The aim of this study was to investigate CS1 gene sequences, protein structure, function and its expression in different tissues, so as to predict its association with meat quality traits. Calsarcins (Calcineurinassociated sarcomeric protein,CS)is a family of calciumbinding proteins,CS1 gene is closely related with meat quality in livestock and poultry. Based on the bovine, Mus musculus and Homo sapiens CS1 mRNA sequences, the cDNA of CS1 gene in ovine was amplified successfully by using comparative genomics technology. The results showed that CS1 of ovine was 951 bp in length with an open reading frame (ORF)793 bp in length and encoded 264 amino acids, and there were three conserved domains in amino acids sequences. Bioinformation analysis indicated that its secondary structures mainly were random coil and helical regions, and contained rich hydrophobic regions, certain phosphorylation sites and protein kinase C (PKC) sites. Meanwhile, tissue expression of the CS1 gene in the Semifine wool sheep were analyzed, the results showed that it was expressed in heart and muscle. When the Semifine Wool sheep was at fifteen days old, the expression was the highest in heart, and there was a significant difference between heart and other tissues (P<0.01). Furthermore, the expression of CS1 decreased with the rise of the age and became the highest when the Semifine Wool sheep was at sixty days old in heart. This result could provide scientific basis for improve meat quality traits.

Key words: CS1 gene, cDNA clone, tissue expression, ovine

苏强强;吴登俊. 绵羊CS-1基因的克隆及在凉山半细毛羊不同组织中的表达[J]. 畜牧兽医学报, 2011, 42(11): 1532-1536.

SU Qiangqiang;WU Dengjun . Cloning of CS-1 in Ovine and Tissue Expression in Liangshan Semifine Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(11): 1532-1536.

[1]	滑留帅, 辛晓玲, 王璟, 罗生金, 曾滔, 巴提玛, 施巧婷, 徐照学, 王二耀. 利用混池重测序鉴定萨福克绵羊超数排卵关键调控基因[J]. 畜牧兽医学报, 2019, 50(9): 1813-1821.
[2]	宋慧子, 栾兆进, 王兆琛, 杜炜, 赵勇超, 张家新. 永生化绵羊附睾上皮细胞系的建立及其生物学特性分析[J]. 畜牧兽医学报, 2019, 50(9): 1822-1831.
[3]	刘家鑫, 魏霞, 邓天宇, 谢锐, 韩建林, 杜立新, 赵福平, 王立贤. 绵羊全基因组ROH检测及候选基因鉴定[J]. 畜牧兽医学报, 2019, 50(8): 1554-1566.
[4]	温东旭, 张雷, 索朗斯珠, 牛家强, 王玉恒, 贡嘎, 旦巴次仁, 席广银, 郭敏, 徐业芬. 牦牛Nramp1基因mRNA组织表达谱及其相关miRNAs初步研究[J]. 畜牧兽医学报, 2019, 50(8): 1576-1586.
[5]	彭孝坤, 张宇, 黄晓瑜, 周广琛, 邢晓南, 张恩平. 急性冷应激对绵羊免疫功能和不同组织热休克蛋白70家族基因表达的影响[J]. 畜牧兽医学报, 2019, 50(8): 1625-1634.
[6]	刘家鑫, 魏霞, 邓天宇, 谢锐, 韩建林, 杜立新, 赵福平, 王立贤. 绵羊全基因组ROH检测及候选基因鉴定[J]. 畜牧兽医学报, 2019, 50(8): 1554-1566.
[7]	温东旭, 张雷, 索朗斯珠, 牛家强, 王玉恒, 贡嘎, 旦巴次仁, 席广银, 郭敏, 徐业芬. 牦牛Nramp1基因mRNA组织表达谱及其相关miRNAs初步研究[J]. 畜牧兽医学报, 2019, 50(8): 1576-1586.
[8]	彭孝坤, 张宇, 黄晓瑜, 周广琛, 邢晓南, 张恩平. 急性冷应激对绵羊免疫功能和不同组织热休克蛋白70家族基因表达的影响[J]. 畜牧兽医学报, 2019, 50(8): 1625-1634.
[9]	巩建飞, 岳静伟, 赵玲玲, 刘运鹏, 王立贤, 刘欣, 孙金海. 猪HMGA1基因的组织表达及其多态性与背膘厚的关联分析[J]. 畜牧兽医学报, 2019, 50(7): 1340-1346.
[10]	宋鹏琰, 李佳丽, 苗艳平, 李相运, 周荣艳, 锡建中. 绵羊miR-150启动子的鉴定及生殖激素对其转录调控研究[J]. 畜牧兽医学报, 2019, 50(7): 1358-1366.
[11]	解领丽, 李嫒, 黄万龙, 张秀秀, 苗向阳. 湖羊卵巢不同发育阶段的miRNA鉴定与分析[J]. 畜牧兽医学报, 2019, 50(7): 1396-1404.
[12]	巩建飞, 岳静伟, 赵玲玲, 刘运鹏, 王立贤, 刘欣, 孙金海. 猪HMGA1基因的组织表达及其多态性与背膘厚的关联分析[J]. 畜牧兽医学报, 2019, 50(7): 1340-1346.
[13]	宋鹏琰, 李佳丽, 苗艳平, 李相运, 周荣艳, 锡建中. 绵羊miR-150启动子的鉴定及生殖激素对其转录调控研究[J]. 畜牧兽医学报, 2019, 50(7): 1358-1366.
[14]	解领丽, 李嫒, 黄万龙, 张秀秀, 苗向阳. 湖羊卵巢不同发育阶段的miRNA鉴定与分析[J]. 畜牧兽医学报, 2019, 50(7): 1396-1404.
[15]	胡亮, 孙伟, 马月辉. 藏系绵羊群体遗传多样性及遗传结构分析[J]. 畜牧兽医学报, 2019, 50(6): 1145-1153.