鹅源新城疫病毒HN基因“自杀性”DNA疫苗的构建及其免疫效果的研究

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (7): 842-846.

鹅源新城疫病毒HN基因“自杀性”DNA疫苗的构建及其免疫效果的研究

张彦红，樊惠英，蔡绍鑫，罗开健，辛朝安，廖明*，任涛*

华南农业大学兽医学院农业部动物疫病防控重点开放实验室，广州 510642

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-20 发布日期:2010-07-20

Construction and Immunity Response of a Suicidal DNA Vaccine EncodingHaemagglutinin Neuraminidase (HN) of a Newcastle Disease Virus Isolated from Goose

ZHANG Yan-hong, FAN Hui-ying, CAI Shao-xin, LUO Kai-jian,XIN Chao-an, LIAO Ming*, REN Tao*

Key Laboratory for Prevention and Control of Animal Diseases of the Ministry of Agriculture,College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20

摘要/Abstract

摘要： 为了探讨鹅源新城疫病毒 “自杀性”DNA疫苗的免疫效果，将鹅源新城疫病毒HN基因片段克隆到自杀性DNA 疫苗载体pSCA1中，构建了表达HN基因的自杀性DNA 疫苗质粒pS-HN。间接免疫荧光试验结果证实，该质粒转染BHK-21细胞后实现了HN基因在该细胞中的表达。将该疫苗和常规DNA 疫苗(pc-HN)分别免疫鹅，结果表明，在诱导体液、细胞免疫和攻毒保护方面，pS-HN均优于pc-HN，这为进一步评价该疫苗的免疫保护效果奠定了基础。

关键词: 鹅源新城疫病毒, HN基因, “自杀性”DNA疫苗

Abstract: To evaluate the immune efficacy of the suicidal DNA vaccine against gooseorigin Newcastle disease, a suicidal DNA vaccine plasmid expressing HN gene of goose-origin Newcastle disease virus was constructed by inserting the HN gene fragment into the expression vector pSCA1, and named as pS-HN. The plasmid was then transfected into BHK-21 cells and the HN protein expression was demonstrated by indirect immunofluorescence assay. Animal immune experiment was conducted by inoculating goose with the pS-HN. The pc-HN, convention DNA vaccine expressing the same HN gene, pcDNA3.1(+) and pSCA1 were used as control. The results revealed that pS-HN was superior to pc-HN in inducing humoral immune response, cellular immune response and have better protective rate against goose-origin Newcastle disease virus. The study laid a foundation for evaluating the protective efficacy of pS-HN.

Key words: goose-origin Newcastle disease virus, HN gene, suicidal DNA vaccines

张彦红;樊惠英;蔡绍鑫;罗开健;辛朝安;廖明;任涛. 鹅源新城疫病毒HN基因“自杀性”DNA疫苗的构建及其免疫效果的研究[J]. 畜牧兽医学报, 2010, 41(7): 842-846.

ZHANG Yan-hong;FAN Hui-ying;CAI Shao-xin;LUO Kai-jian;XIN Chao-an;LIAO Ming;REN Tao. Construction and Immunity Response of a Suicidal DNA Vaccine EncodingHaemagglutinin Neuraminidase (HN) of a Newcastle Disease Virus Isolated from Goose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(7): 842-846.

[1]	张彦红;樊惠英;蔡绍鑫;罗开健;辛朝安;廖明;任涛. 鹅源新城疫病毒HN基因“自杀性”DNA疫苗的构建及其免疫效果的研究[J]. 畜牧兽医学报, 2010, 41(7): 842-846.
[2]	徐怀英;秦卓明;王友令;欧阳文军;谭雷涛;何叶峰;金维江;袁小远. 中国水禽新城疫病毒F和HN基因的变异趋势[J]. 畜牧兽医学报, 2009, 40(3): 394-401.
[3]	徐怀英;秦卓明;王友令;欧阳文军;谭雷涛;何叶峰;金维江;袁小远. 中国水禽新城疫病毒F和HN基因的变异趋势[J]. 畜牧兽医学报, 2009, 40(3): 394-401.
[4]	谭雷涛;秦卓明;马保臣;徐怀英;崔治中. 一株新城疫重组强毒的分离鉴定与分子特性分析[J]. 畜牧兽医学报, 2007, 38(11): 1217-1223.
[5]	谭雷涛;秦卓明;马保臣;徐怀英;崔治中. 一株新城疫重组强毒的分离鉴定与分子特性分析[J]. 畜牧兽医学报, 2007, 38(11): 1217-1223.