DRD2基因14个多态位点与鸡产蛋性状的相关性

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (8): 944-950.

DRD2基因14个多态位点与鸡产蛋性状的相关性

徐海平¹，周敏²，方梅霞^1,3，曾华¹，聂庆华¹，张德祥¹，张细权¹*

1. 华南农业大学动物科学学院，广州 510642； 2. 江西教育学院生物技术研究所，南昌 330029； 3.暨南大学医学院实验动物管理中心，广州 510632

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-08-20 发布日期:2010-08-20
通讯作者: 张细权

Association of 14 Polymorphic Sites of the DRD2 Gene with Chicken Egg Laying Traits

XU Hai-ping¹, ZHOU Min², FANG Mei-xia^{1, 3}, ZENG Hua¹, NIE Qing-hua¹,
ZHANG De-xiang¹, ZHANG Xi-quan¹*

1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;2. Department of Biological Technology, Jiangxi Institute of Education, Nanchang 330029, China;3. Department of Laboratory Animal Science, Medical College of Jinan University, Guangzhou 510632, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

摘要/Abstract

摘要： 本研究旨在分析DRD2基因与鸡产蛋性状的相关性，寻找可作为鸡产蛋性状的重要标记。在该基因上选取了14个多态位点，采用PCR-RFLP及PCR测序等方法在宁都三黄鸡母系644个个体中进行了鸡产蛋性状的关联分析。结果表明：位于该基因5′调控区的4个突变位点G38560C、T-38326G、T-32751C和A-16105G与鸡的开产日龄显著或极显著（P<0.05或P<0.01）相关，位点A-16105G还与300日龄总产蛋数显著相关（P<0.05）；由G38560C、G-38544A、I-38463D与T-38326G这4个位点组成的单倍型块与开产日龄呈极显著相关（P=0.006 5<0.01）。研究结果提示：G-38560C、T-38326G、T-32751C和 A-16105G这4个突变位点以及由G-38560C、G-38544A、I-38463D与T-38326G组成的单倍型块可作为产蛋性状标记辅助选择的有效分子标记。

关键词: DRD2基因, 多态位点, 单倍型, 产蛋性状

Abstract: The purpose of this study was to analyze the associations of the DRD2 gene with chicken egg laying traits and find important markers for these traits. Fourteen polymorphic sites of this gene were selected, and the association analysis was performed in 644 chickens of Ningdu Sanhuang female line by PCR-RFLP and sequencing methods. The results showed that 4 mutation sites in the 5′regulatory region of the DRD2 gene, G-38560C, T-38326G, T-32751C and A-16105G, were significantly associated with the age of first egg (AFE) (P<0.05 or P<0.01). A-16105G was also significantly associated with total egg number at 300 days (EN300) (P<0.05). The haplotype block consisting of G-38560C, G-38544A, I-38463D and T-38326G was significantly associated with AFE (P<0.05). It was suggested that G-38560C, T-38326G, T-32751C and A-16105G on the DRD2 gene and the haplotype block consisting of G38560C, G-38544A, I-38463D and T-38326G could be used as effective molecular markers in marker assisted selection (MAS) for egg laying traits.

Key words: DRD2 gene, polymorphic site, haplotype, egg laying trait

徐海平;周敏;方梅霞;曾华;聂庆华;张德祥;张细权. DRD2基因14个多态位点与鸡产蛋性状的相关性[J]. 畜牧兽医学报, 2010, 41(8): 944-950.

XU Hai-ping;ZHOU Min;FANG Mei-xia;;ZENG Hua;NIE Qing-hua;ZHANG De-xiang;ZHANG Xi-quan. Association of 14 Polymorphic Sites of the DRD2 Gene with Chicken Egg Laying Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(8): 944-950.

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