关键词: 噻环乙胺, AC, cAMP, 分子机理, 信号转导

Abstract: To observe the dynamic effects of tiletamine on AC activity and cAMP content in different brain regions of rats, 168 SD rats were randomly divided into two groups, and the activity of AC and the cAMP content were measured respectively. Every group was divided into control group, high dose group(ip tilelamine 60 mg·kg^-1) and low dose group(ip tilelamine 30 mg·kg^-1). The group was divided into three subgroups of maintenance of anesthesia, recovery from anesthesiaⅠand recovery from anesthesiaⅡ according to their anesthesia state. The activity of AC and the cAMP content were measured by radioimmunoassay (RIA). The results of experiment showed that in the high and low dose maintenance of anesthesia groups, the AC ctivity in cerebral cortex and thalamus were obviously increased, and the cAMP content in abovementioned regions were also obviously increased(compared with the control group, P<0.05). In the recovery from anesthesiaⅠgroups of high dose group and low dose group, the activity of AC and the cAMP content were decreased at different degree, and the high dose group was decreased obviously(compared with maintenance of anesthesia group, P<0.01). In the recovery from anesthesiaⅡ, they were obviously decreased(compared with maintenance of anesthesia group, P<0.01). Two dose groups have insignificant effect on the AC activity and the cAMP content in hippocampus, brain stem, and cerebellum. The results suggested that the AC activity and the cAMP content might play an important part in the molecular mechanisms of general anesthesia mediated by tiletamine. The anesthetic action of tiletamine might relate to enchance the activity of AC and increase the cAMP content in the cerebral cortex and thalamus.

Key words: tiletamine, AC, cAMP, molecular mechanism, signal transduction